"Rosettes" Protocols and Video Articles

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Francesca Pistollato¹, David Canovas-Jorda¹, Dimitra Zagoura¹, Anna Price¹

¹Directorate F - Health, Consumers and Reference Materials, Joint Research Centre

A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases

Aaron Topol¹, Ngoc N. Tran¹, Kristen J. Brennand^1,2

¹Department of Psychiatry, Icahn School of Medicine at Mount Sinai, ²Department of Neuroscience, Icahn School of Medicine at Mount Sinai

JoVE 52495

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Nadja Zeltner¹, Fabien G. Lafaille², Faranak Fattahi¹, Lorenz Studer¹

¹Developmental Biology, Center for Stem Cell Biology, Sloan-Kettering Institute for Cancer Research, ²St. Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University

JoVE 51609

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

Max R Salick*¹, Michael F Wells*^2,3, Kevin Eggan^2,3, Ajamete Kaykas¹

¹Department of Neuroscience, Novartis Institutes for BioMedical Research, ²Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, ³Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University

JoVE 56404

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Pengbo Zhang*¹, Ninuo Xia*¹, Renee A. Reijo Pera^1,2

¹Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, ²Department of Obstetrics and Gynecology, Stanford University School of Medicine

JoVE 51737

ES Cell-derived Neuroepithelial Cell Cultures

Shreeya Karki¹, Jan Pruszak¹, Ole Isacson¹, Kai C Sonntag¹

¹McLean Hospital, Harvard Medical School

JoVE 118

Analysis of Arabidopsis thaliana Growth Behavior in Different Light Qualities

Bettina Bölter¹, Franka Seiler¹, Jürgen Soll¹

¹Department Biologie I-Botanik, Ludwig-Maximilians-Universität

JoVE 57152

Structural Design and Manufacturing of a Cruiser Class Solar Vehicle

Giangiacomo Minak^1,2,3, Tommaso M. Brugo¹, Cristiano Fragassa^1,2, Ana Pavlovic², Felipe V. de Camargo², Nicola Zavatta³

¹Department of Industrial Engineering, Università di Bologna, ²CIRI MAM, Università di Bologna, ³CIRI AERO, Università di Bologna

Video Coming Soon

JoVE 58525

An Easy Method for Plant Polysome Profiling

Cécile Lecampion^1,2,3, Maïna Floris^1,2,3,4, Jean Raphaël Fantino^5,6, Christophe Robaglia^1,2,3, Christophe Laloi^1,2,3

¹Laboratoire de Génétique et Biophysique des Plantes, Aix-Marseille Université, ²UMR 7265 Biologie Végétale & Microbiologie Environnementales, CNRS, ³BIAM, CEA, ⁴Department of Biology, Biocenter, University of Copenhagen, ⁵Laboratoire de Chimie Bactérienne, ⁶CNRS, LCB UMR 7283, Aix Marseille Université

JoVE 54231

Application of Two-spotted Spider Mite Tetranychus urticae for Plant-pest Interaction Studies

Marc Cazaux*^1,2, Marie Navarro*^1,2, Kristie A. Bruinsma*¹, Vladimir Zhurov*¹, Tara Negrave¹, Thomas Van Leeuwen^3,4, Vojislava Grbic^1,2, Miodrag Grbic^1,2

¹Department of Biology, The University of Western Ontario, ²Instituto de Ciencias de la Vid y el Vino, ³Department of Crop Protection, Ghent University, ⁴Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam

JoVE 51738

Generation of iPSC-derived Human Brain Organoids to Model Early Neurodevelopmental Disorders

Elke Gabriel¹, Jay Gopalakrishnan^1,2

¹Center for Molecular Medicine Cologne, University of Cologne, ²Institute for Biochemistry I, Medical School of University of Cologne

JoVE 55372

Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays

Andre W. Phillips¹, Jonathan E. Nestor¹, Michael W. Nestor¹

¹The Hussman Institute for Autism

JoVE 55799

In Vivo Imaging of Cx3cr1^gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy

Despina Kokona¹, Joël Jovanovic¹, Andreas Ebneter¹, Martin S. Zinkernagel¹

¹Department of Ophthalmology and Department of Clinical Research, Bern University Hospital and University of Bern

JoVE 55984

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

Dumizulu L. Tembo¹, Jacqui Montgomery¹, Alister G. Craig², Samuel C. Wassmer³

¹Malawi-Liverpool-Wellcome Trust Clinical Research Programme, ²Liverpool School of Tropical Medicine, ³Department of Microbiology, Division of Medical Parasitology, New York University School of Medicine

JoVE 4316

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

David J. Tischfield^1,2, Stewart A. Anderson¹

¹Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, ²Department of Psychiatry, Children's Hospital of Philadelphia, University of Pennsylvania School of Medicine

JoVE 56358

Streamlined 3D Cerebellar Differentiation Protocol with Optional 2D Modification

Dwayne B. Holmes¹, Vivi M. Heine^1,2

¹Department of Pediatrics/Child Neurology, Amsterdam Neuroscience, VU University Medical Center, ²Department of Complex Trait Genetics, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam

JoVE 56888

Biochemical Titration of Glycogen In vitro

Joffrey Pelletier¹, Grégory Bellot¹, Jacques Pouysségur¹, Nathalie M. Mazure¹

¹Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 - INSERM, U1081 - UNS, Centre Antoine Lacassagne, University of Nice - Sophia Antipolis

JoVE 50465

2D and 3D Matrices to Study Linear Invadosome Formation and Activity

Julie Di Martino*^1,2, Elodie Henriet*¹, Zakaria Ezzoukhry^1,3,4, Chandrani Mondal², Jose Javier Bravo-Cordero², Violaine Moreau¹, Frederic Saltel¹

¹INSERM U1053, ²Icahn School of Medicine at Mount Sinai, Tisch Cancer Institute, ³Faculté de Médecine, Casablanca, Université Mohammed 6 des Sciences de la Santé (UM6SS), ⁴Laboratoire National de Référence

JoVE 54911

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Sandra Meyer^1,2, Philipp Wörsdörfer¹, Katharina Günther¹, Marc Thier^2,3, Frank Edenhofer^1,2

¹Institute of Anatomy and Cell Biology, University of Würzburg, ²Institute of Reconstructive Neurobiology, University of Bonn, ³German Cancer Research Center, Heidelberg

JoVE 52831

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Vishal Menon¹, Ria Thomas^1,2, Arun R. Ghale^1,3, Christina Reinhard¹, Jan Pruszak^1,4

¹Emmy Noether-Group for Stem Cell Biology, Department of Molecular Embryology, Institute of Anatomy and Cell Biology, University of Freiburg, ²Spemann Graduate School of Biology and Medicine and Faculty of Biology, University of Freiburg, ³School of Life Sciences, Keele University, ⁴Center for Biological Signaling Studies (BIOSS), University of Freiburg

JoVE 52241

An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach

Sachin Parikh¹, Andrew Le¹, Julian Davenport¹, Michael B. Gorin¹, Steven Nusinowitz¹, Anna Matynia¹

¹Jules Stein Eye Institute, University of California, Los Angeles

JoVE 54808

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

Imen Bouchnak¹, Lucas Moyet¹, Daniel Salvi¹, Marcel Kuntz¹, Norbert Rolland¹

¹Université Grenoble Alpes, INRA, CNRS, CEA

Video Coming Soon

JoVE 58581

Methods Collection

The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example

Brendan M. O'Leary¹, Arantza Rico², Sarah McCraw¹, Helen N. Fones³, Gail M. Preston¹

¹Department of Plant Sciences, University of Oxford, ²School of Education of Vitoria-Gasteiz, University of the Basque Country (UPV/EHU), ³Biosciences, University of Exeter

JoVE 52113

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Yan Xing¹, Kristin A. Hogquist¹

¹Department of Laboratory Medicine & Pathology, Center for Immunology, University of Minnesota

JoVE 51780

Efficient Derivation of Human Neuronal Progenitors and Neurons from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

Xuejun H. Parsons^1,2, Yang D. Teng^3,4, James F. Parsons^1,2, Evan Y. Snyder^1,2,5, David B. Smotrich^1,2,6, Dennis A. Moore^1,2

¹San Diego Regenerative Medicine Institute, ²Xcelthera, ³Department of Neurosurgery, Harvard Medical School, ⁴Division of SCI Research, VA Boston Healthcare System, ⁵Program in Stem Cell & Regenerative Biology, Sanford-Burnham Medical Research Institute, ⁶La Jolla IVF

JoVE 3273

In vivo Electroporation of Developing Mouse Retina

Jimmy de Melo¹, Seth Blackshaw^1,2,3,4,5

¹Solomon H. Snyder Department of Neuroscience, Johns Hopkins School of Medicine, ²Department of Neurology, Johns Hopkins School of Medicine, ³Department of Ophthalmology, Johns Hopkins School of Medicine, ⁴Center for High-Throughput Biology, Johns Hopkins School of Medicine, ⁵Institute for Cell Engineering, Johns Hopkins School of Medicine

JoVE 2847

Propagation of Human Embryonic Stem (ES) Cells

Laurence Daheron¹

¹Center for Regenerative Medicine, MGH - Massachusetts General Hospital

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