Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
1Department of Biochemistry, University of Leicester
Biology
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Biology
Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
Bioengineering
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen
Immunology and Infection
Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
1Department of Biological Sciences, SUNY Oswego, 2Department of Cell Biology, Johns Hopkins University School of Medicine
Biology
An Orbital Shaking Culture of Mammalian Cells in O-shaped Vessels for the Production of Uniform Aggregates
1Department of Chemical System Engineering, University of Tokyo, 2Department of Biotechnology, Osaka University, 3Biotech Business Unit, Fukoku Co. Ltd, 4Institute of Industrial Science, University of Tokyo
JoVE In-Press
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
1Program in Molecular Medicine, The Hospital for Sick Children Research Institute, 2Department of Biochemistry, University of Toronto, 3Department of Immunology, University of Toronto
Biochemistry
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
1Dept. of Medical Biology, University of Québec
Biology
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
1Molecular Pharmacology and Pathology Program, Department of Pathology & Bosch Institute, University of Sydney, 2Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University
Biology
CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
Bioengineering
Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
1Nemours Biomedical Research, Alfred I. duPont Hospital for Children
Biology
Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea
Developmental Biology
Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
1Langone Medical Center, New York University
Biochemistry
Expression and Purification of Mammalian Bestrophin Ion Channels
1Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry
Biochemistry
Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
1Department of Medicine, Hematology-Oncology, University of California, Los Angeles, 2Department of Biological Chemistry, University of California, Los Angeles
Biology
A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
1London Research and Development Centre, Agriculture & Agri-Food Canada, 2Department of Biology, University of Western Ontario, 3Department of Microbiology and Immunology, University of Western Ontario
Biology
Isolation, Culture, and Imaging of Human Fetal Pancreatic Cell Clusters
1Pediatric Diabetes Research Center, Department of Pediatrics, University of California, San Diego
Medicine
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
1Nuclear Dynamics Programme, The Babraham Institute, Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute, Babraham Research Campus, 5Department of Biological Science, Florida State University
Genetics
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
1Department of Biomedical Engineering, School of Medicine, Tsinghua University, 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, 3School of Life Sciences, Tsinghua University
Bioengineering
Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo, 2K. G. Jebsen Centre for B cell malignancies and K. G. Jebsen Centre for Cancer Immunotherapy, University of Oslo
JoVE In-Press
Laboratory Scale Production and Purification of a Therapeutic Antibody
1Faculty of Pharmacy, University of Sydney
Biochemistry
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
1Department of Chemistry and Applied Biosciences, ETH Zurich, Switzerland
Bioengineering
Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
1College of Life Sciences, South China Agricultural University
Chemistry
Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
1Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina
Developmental Biology
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
1Institute for Biochemistry and Biology, Cell Membrane Biophysics Group, University of Potsdam
JoVE In-Press
Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
1Tumour Virology Division F010, German Cancer Research Center (DKFZ), 2Inserm Unit 701, German Cancer Research Center (DKFZ)
Immunology and Infection
Cellular Redox Profiling Using High-content Microscopy
1Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, 2Cell Systems and Imaging Research Group (CSI), Department of Molecular Biotechnology, Ghent University, 3Department of Biochemistry , Radboud Institute for Molecular Life Sciences, Radboud University Medical Center
Biology
Rapid Isolation of the Mitoribosome from HEK Cells
1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University
JoVE In-Press
Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
1Harvard Medical School, 2Division of Hematology/Oncology, Boston Children's Hospital, 3Department of Pediatric Oncology, Dana-Farber Cancer Institute, 4Howard Hughes Medical Institute
Biology
Electroporation of Functional Bacterial Effectors into Mammalian Cells
1Biological Sciences Division, Pacific Northwest National Laboratory, 2Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, 3Structural Proteomics Group, Ontario Center for Structural Proteomics, University of Toronto, 4Center for Bioproducts and Bioenergy, Washington State University
Immunology and Infection
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
1Department of Thoracic and Cardiovascular Surgery and Inter-University Centre for Medical Technology Stuttgart-Tübingen (IZST), Eberhard Karls University, Tübingen, 2Department of Cell and Tissue Engineering, Fraunhofer Institute of Interfacial Engineering and Biotechnology (IGB) Stuttgart, Germany, 3Department for Medical Interfacial Engineering (IGVT), University of Stuttgart, Germany, 4Institute of Tissue Engineering and Regenerative Medicine, Julius-Maximillians University, Würzburg, Germany
Bioengineering
Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
1Department of Infectious Diseases, The Scripps Research Institute, 2Department of Chemistry, City College of New York
Biology
Abcam Quantitative Cleaved PARP-1 High-Throughput In-Cell ELISA (ICE) Assay - ADVERTISEMENT
1Abcam, plc
Application Notes
Neo-Islet Formation in Liver of Diabetic Mice by Helper-dependent Adenoviral Vector-Mediated Gene Transfer
1Department of Medicine, Baylor College of Medicine, 2Division of Diabetes, Endocrinology & Metabolism, Diabetes & Endocrinology Research Center, Baylor College of Medicine, 3Department of Molecular & Cellular Biology, Baylor College of Medicine
Medicine
Power Input Measurements in Stirred Bioreactors at Laboratory Scale
1Finesse, Thermo Fisher Scientific, 2Institute of Chemistry and Biotechnology, Zurich University of Applied Sciences, School of Life Sciences and Facility Management
Bioengineering
Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
1Max von Pettenkofer-Institut & Genzentrum, Virologie, Nationales Referenzzentrum für Retroviren, Medizinische Fakultät, LMU München, 2Institut für Laboratoriumsmedizin, klinische Chemie und Pathobiochemie, Charité - Universitätsmedizin Berlin, 3Institut für Physiologische Chemie, Martin-Luther-Universität Halle-Wittenberg
Bioengineering
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
1Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering, Eindhoven University of Technology, 2Institute for Immunity, Transplantation, and Infection, Beckman Center, Stanford University
JoVE In-Press
High Throughput In Vitro Assessment of Latency Reversing Agents on HIV Transcription and Splicing
1Department of Microbiology and Immunology, University of Melbourne
JoVE In-Press
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
Immunology and Infection
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Biochemistry
Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Immunology and Infection
Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
1Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán
JoVE In-Press
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
1Department of Pathology, Stanford University School of Medicine, 2Department of Microbiology & Immunology, Stanford University School of Medicine
Immunology and Infection
Chromatin Immunoprecipitation from Human Embryonic Stem Cells
1Department of Biochemistry, University of California - Riverside
Biology
Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
1Center for Biotechnology Education, Johns Hopkins University, 2PBS Biotech, Inc.
Bioengineering
Production and Detection of Reactive Oxygen Species (ROS) in Cancers
1Center for Cell and Gene Therapy, Baylor College of Medicine
Medicine
Micropipette Aspiration of Substrate-attached Cells to Estimate Cell Stiffness
1Section of Respiratory, Critical Care and Sleep Medicine, Department of Medicine, University of Illinois, 2Institute for Medicine and Engineering, University of Pennsylvania
Bioengineering
Isolation and Detection of Telomeric DNA C-Circles from Mammalian Cells
1Department of Pharmacology & Experimental Therapeutics, Boston University
JoVE In-Press
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
1Gene Expression Division, Bio-Rad Laboratories
Biology
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