Show Advanced Search

REFINE YOUR SEARCH:

Containing Text
- - -
+
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by journal section

Filter by science education

 
 
Abdominal Cavity: The region in the abdomen extending from the thoracic Diaphragm to the plane of the superior pelvic aperture (pelvic inlet). The abdominal cavity contains the Peritoneum and abdominal Viscera, as well as the extraperitoneal space which includes the Retroperitoneal space.

Abdominal Exam I: Inspection and Auscultation

JoVE 10088

Source: Alexander Goldfarb, MD, Assistant Professor of Medicine, Beth Israel Deaconess Medical Center, MA


Gastrointestinal disease accounts for millions of office visits and hospital admissions annually. Physical examination of the abdomen is a crucial tool in diagnosing diseases of the gastrointestinal tract; in addition, it can help…

 Physical Examinations II

Abdominal Exam II: Percussion

JoVE 10090

Source: Alexander Goldfarb, MD, Assistant Professor of Medicine, Beth Israel Deaconess Medical Center, MA


Medical percussion is based on the difference in pitch between the sounds elicited by tapping on the body wall. The auditory response to tapping depends on the ease with which the body wall vibrates, and is influenced by underlying…

 Physical Examinations II

Needle Thoracostomy

JoVE 10233

Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA


A tension pneumothorax is a life-threatening situation in which excess air is introduced into the pleural space surrounding the lung, either through trauma to the chest cavity or as a spontaneous leak of air from the lung itself. Air…

 Emergency Medicine and Critical Care

Oogenesis

JoVE 10906

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal distribution of cell contents ensures that there are enough cytoplasm and nutrients to nourish the early stages of development. Second, during oogenesis, meiosis “arrests” at two distinct points: once during embryonic growth and a second time during puberty. In mammals, oocytes are suspended in prophase I until sexual maturation, at which point meiosis I continues under hormonal influence until an egg precursor cell is released into a fallopian tube. At ovulation, the precursor exits the ovary and, only if fertilization occurs, is stimulated to complete meiosis II and form a complete egg. Defects during oogenesis can result in severe consequences. In particular, problems with chromosome segregation during either meiosis I or meiosis II may lead to an embryo being aneuploid, meaning that it contains an abnormal number of chromosomes. Increased age elevates a woman

 Core: Biology

Diagnostic Necropsy and Tissue Harvest

JoVE 10294

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN


Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of…

 Lab Animal Research

Sterile Tissue Harvest

JoVE 10298

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN


In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research.1 The …

 Lab Animal Research

Considerations for Rodent Surgery

JoVE 10285

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN


The Guide for the Care and Use of Laboratory Animals1 dictates that rodent survival surgery be performed aseptically. Aseptic technique utilizes specific practices that minimize the contamination of the surgical site, including…

 Lab Animal Research

Murine In Utero Electroporation

JoVE 5208

In utero electroporation is an important technique for studying the molecular mechanisms that guide the proliferation, differentiation, migration, and maturation of cells during neural development. Electroporation enables the rapid and targeted delivery of material into cells by utilizing electrical pulses to create transient pores in cell membranes. Although…

 Neuroscience

Blood Withdrawal I

JoVE 10246

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN


Blood collection is a common requirement for research studies that involve mice and rats. The method of blood withdrawal in mice and rats is dependent upon the volume of blood needed, the frequency of the sampling, the health status of the …

 Lab Animal Research

Anatomy of the Intestines

JoVE 10830

Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occur in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.

The small intestine is an ~22 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the small intestine is highly convoluted and covered in finger-like extensions called villi, each containing hundreds of microvilli. The folds, villi, and microvilli of the small intestine amplify the surface area of absorption 60 to 120 times. The increased surface area provides ample opportunity for nutrients to be absorbed. The small intestine connects to the stomach by the pyloric sphincter, which closes off when chyme moves into the duodenum—the beginning of the small intestine. The middle and largest part of the small intestine is the jejunum. The ileum ends the small intestine, where it attaches to the large intestine by the ileocecal valve. The large intestine starts at the cecum. The appendix, a small lymphatic structure, dangles from the bottom of the cecum. Above the cecum, starts the ascending colon followed by the transverse colon. They absorb most of the remaining

 Core: Biology

Cranial Nerves Exam II (VII-XII)

JoVE 10005

Source:Tracey A. Milligan, MD; Tamara B. Kaplan, MD; Neurology, Brigham and Women's/Massachusetts General Hospital, Boston, Massachusetts, USA


The cranial nerve examination follows the mental status evaluation in a neurological exam. However, the examination begins with observations made upon greeting…

 Physical Examinations III

Biodegradable Magnesium Stent Treatment of Saccular Aneurysms in a Rat Model - Introduction of the Surgical Technique

1Department of Neurosurgery, Kantonsspital Aarau, 2Neuro Lab, Research Group for Experimental Neurosurgery and Neurocritical Care, Department of Intensive Care Medicine, University Hospital and University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau

JoVE 56359

 Neuroscience

In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments

1Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, 2Visceral Pain Group, Discipline of Medicine, University of Adelaide, 3Department of Biomedical Sciences, University of Sheffield, 4Department of Pharmacy, Pharmacology and Postgraduate Medicine, University of Hertfordshire, 5Department of Gastroenterology and Hepatology, Antwerp University Hospital

JoVE 54576

 Neuroscience

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

1Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute, 3Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute

JoVE 55095

 Medicine

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia

Video Coming Soon

JoVE 60992

 JoVE In-Press

Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

1Department of Thoracic Surgery, The First Hospital of Jilin University, 2The Skaggs School of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, 3Key Laboratory of Zoonoses Research, Ministry of Education, Jilin University, 4Department of Cardiology, China-Japan Union Hospital of Jilin University, 5Department of Gastrointestinal, Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University

Video Coming Soon

JoVE 60847

 JoVE In-Press

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

1Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, 2Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles, 3Department of Bioengineering, Hanyang University, 4Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 5Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, 6School of Life Sciences, Beijing Normal University, 7Department of Urology, West China Hospital, Sichuan University, 8Department of Urology, David Geffen School of Medicine, University of California, Los Angeles

JoVE 60314

 Cancer Research

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

1Department of Human Anatomy and Cell Science, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 2The Children's Hospital Research Institute of Manitoba (CHRIM), Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, 3Department of Oral Biology, University of Illinois at Chicago, 4Department of Pathology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba

JoVE 60168

 Neuroscience

Assessment of Sexual Behavior of Male Mice

1Department of Psychiatry and Mental Health Institute of the Second Xiangya Hospital, Central South University, National Clinical Research Center on Mental Disorders and National Technology Institute on Mental Disorders, Hunan Key Laboratory of Psychiatry and Mental Health, 2Research Center for Pharmacology & Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, 3Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University

Video Coming Soon

JoVE 60154

 JoVE In-Press

Ultrasound-Guided Orthotopic Implantation of Murine Pancreatic Ductal Adenocarcinoma

1Department of Medicine, Perelman School of Medicine, University of Pennsylvania, 2Pancreatic Cancer Mouse Hospital, Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, 4Parker Institute for Cancer Immunotherapy, Perelman School of Medicine, University of Pennsylvania

JoVE 60497

 Cancer Research

Murine Appendectomy Model of Chronic Colitis Associated Colorectal Cancer by Precise Localization of Caecal Patch

1Department of General Surgery, The First Affiliated Hospital, Xi'an Jiaotong University, 2Institute of Medical Research, Northwestern Polytechnical University, 3School of Life Sciences, Northwestern Polytechnical University, 4Department of Medicine, Xi'an Jiaotong University, 5Princess Margaret Cancer Center, University of Health Network, 6Department of Pathology, Nanjing Medical University

JoVE 59921

 Medicine

Porcine Model of Infrarenal Abdominal Aortic Aneurysm

1Department of Surgery, University of Virginia School of Medicine, 2Department of Surgery, Ochsner Medical Center, 3Department of Surgery, University of Florida, 4The Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

JoVE 60169

 Medicine

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

1West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, 2Department of Biomedical Sciences, School of Medicine and Health Sciences, University of North Dakota, 3State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Army Medical University

JoVE 59386

 Immunology and Infection

Renal Subcapsular Transplantation of 2'-Deoxyguanosine-Treated Murine Embryonic Thymus in Nude Mice

1The First Affiliated Hospital, Jinan University, 2Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Jinan University, 3The Institute of Clinical Medicine, Jinan University, 4Joint institute of Metabolic Medicine Between Jinan University and University of Hong Kong

JoVE 59657

 Immunology and Infection

Murine Model of Central Venous Stenosis using Aortocaval Fistula with an Outflow Stenosis

1Department of Surgery, Yale University, 2Vascular Biology and Therapeutics Program, Yale University, 3Department of Vascular Surgery, University of Tokyo, 4Department of Diagnostic Radiology, Tokai University School of Medicine, 5Department of Cardiovascular Surgery, Tokyo Medical University, 6Department of Vascular Surgery, VA Connecticut Healthcare Systems

JoVE 59540

 Medicine
123458
More Results...