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Air Pressure: The force per unit area that the air exerts on any surface in contact with it. Primarily used for articles pertaining to air pressure within a closed environment.

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

1Department of Bioengineering & Therapeutic Sciences, University of California, San Francisco, 2Department of Obstetrics, Gynecology & Reproductive Sciences, University of California, San Francisco, 3Keck School of Medicine, University of Southern California

JoVE 51745


 Bioengineering

Whole-Body Nanoparticle Aerosol Inhalation Exposures

1Department of Physiology and Pharmacology, School of Medicine, West Virginia University, 2Center for Cardiovascular and Respiratory Sciences, West Virginia University, 3National Institute for Occupational Safety and Health

JoVE 50263


 Biology

Conducting Miller-Urey Experiments

1School of Chemistry and Biochemistry, Georgia Institute of Technology, 2Earth-Life Science Institute, Tokyo Institute of Technology, 3Institute for Advanced Study, 4Astromaterials Research and Exploration Science Directorate, NASA Johnson Space Center, 5Goddard Center for Astrobiology, NASA Goddard Space Flight Center, 6Geosciences Research Division, Scripps Institution of Oceanography, University of California at San Diego

JoVE 51039


 Chemistry

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

1Department of Biomedical Engineering, Ohio State University Wexner Medical Center, 2Davis Heart & Lung Research Institute, Ohio State University Wexner Medical Center, 3The Collaboration for Organ Perfusion, Protection, Engineering and Regeneration (COPPER) Laboratory, Ohio State University Wexner Medical Center, 4Division of Cardiac Surgery, Department of Surgery, Ohio State University Wexner Medical Center, 5Departments of Pediatrics and Internal Medicine, Ohio State University, 6Advanced Lung Disease Program, Lung and Heart-Lung Transplant Programs, Nationwide Children's Hospital, 7Division of Transplantation, Department of Surgery, Ohio State University Wexner Medical Center

JoVE 52309


 Medicine

Making Record-efficiency SnS Solar Cells by Thermal Evaporation and Atomic Layer Deposition

1Department of Mechanical Engineering, Massachusetts Institute of Technology, 2Laboratory for Manufacturing and Productivity, Massachusetts Institute of Technology, 3School of Engineering and Applied Sciences, Harvard University, 4Department of Materials Science and Engineering, Massachusetts Institute of Technology, 5Department of Chemistry & Chemical Biology, Harvard University

JoVE 52705


 Engineering

Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats

1Department of Physical Therapy, Marquette University, 2Medical College of Wisconsin, 3Department of Physiology, Medical College of Wisconsin, 4Graduate Programs of Nurse Anesthesia, Texas Wesleyan University, 5Office of Research, Medical College of Wisconsin

Video Coming Soon

JoVE 56133


 JoVE In-Press

Safety Precautions and Operating Procedures in an (A)BSL-4 Laboratory: 1. Biosafety Level 4 Suit Laboratory Suite Entry and Exit Procedures

1Integrated Research Facility at Frederick, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), 2Environmental Health and Safety, Biological and Chemical Safety Program, University of Texas Medical Branch

JoVE 52317


 Immunology and Infection

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Mechanical Engineering, Stanford University, 3Department of Bioengineering, Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology, The Institute of Photonic Sciences (ICFO)

Video Coming Soon

JoVE 56530


 JoVE In-Press

Glovebox and Impurity Sensors

JoVE 10317

Source: Tamara M. Powers, Department of Chemistry, Texas A&M University 

The glovebox provides a straightforward means to handle air- and moisture-sensitive solids and liquids. The glovebox is what it sounds like: a box with gloves attached to one or more sides, which allows the user to perform manipulations within the glovebox under an inert atmosphere. For manipulations under inert atmospheres, chemists can choose between Schlenk or high-vacuum techniques and a glovebox. Schlenk and particularly high-vacuum techniques offer a higher degree of control of the atmosphere, and are thus suitable for reactions that are greatly air- and moisture-sensitive. The glovebox, however, provides greater access for manipulations in an inert atmosphere. Weighing out reagents, filtering reactions, preparing samples for spectroscopy, and growing crystals are all examples of routine procedures that are more readily performed in a glovebox versus a Schlenk/vacuum manifold. Advancements in glovebox design have increased its performance, such as running reactions at reduced temperatures and spectroscopy within the glovebox. This video will demonstrate how to bring items in and out of the glovebox and how to qualitatively ensure a good working environment. Basic manipulations within a glov


 Inorganic Chemistry

Synthesis Of A Ti(III) Metallocene Using Schlenk Line Technique

JoVE 10428

Source: Tamara M. Powers, Department of Chemistry, Texas A&M University 

Inorganic chemists often work with highly air- and water-sensitive compounds. The two most common and practical methods for air-free synthesis utilize either Schlenk lines or gloveboxes. This experiment will demonstrate how to perform simple manipulations on a Schlenk line with a focus on solvent preparation and transfer. Through the synthesis of a reactive Ti(III) metallocene complex, we will demonstrate a new, simple method to degas solvent as well as how to transfer solvent by cannula and by syringe on a Schlenk line. The synthesis of a Ti(III) metallocene compound 3 is shown in Figure 1.1 Compound 3 is highly reactive with O2, (see oxidation of compound 3 to Ti(IV) metallocene 4 shown in Figure 1). Therefore, it is important to run the synthesis under anaerobic conditions. The synthesis of target compound 3 can be monitored visually and progresses through one additional color change before arriving at the desired product, which is blue in color. If during the experiment there is an observed color change from blue to yellow (or green = blue + yellow), this is an indication that O2 entered the flask and that undesired oxidation of compound 3 to


 Inorganic Chemistry

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