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Area Under Curve: A statistical means of summarizing information from a series of measurements on one individual. It is frequently used in clinical pharmacology where the Auc from serum levels can be interpreted as the total uptake of whatever has been administered. As a plot of the concentration of a drug against time, after a single dose of medicine, producing a standard shape curve, it is a means of comparing the bioavailability of the same drug made by different companies. (From Winslade, Dictionary of Clinical Research, 1992)
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 JoVE Medicine

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

1iC42 Clinical Research and Development, University of Colorado, Anschutz Medical Campus, 2Division of Clinical Pharmacology, Cincinnati Children's Hospital Medical Center, 3Food and Drug Administration (FDA), Center of Drug Evaluation Research - Office of Generic Drugs, 4Transplant Clinical Research, University of Cincinnati


JoVE 52424

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 JoVE Chemistry

Quantitative Detection of Trace Explosive Vapors by Programmed Temperature Desorption Gas Chromatography-Electron Capture Detector

1Chemical Sensing & Fuel Technology, Chemistry Division, U.S. Naval Research Laboratory, 2NOVA Research, Inc., 3Bio/Analytical Chemistry, Chemistry Division, U.S. Naval Research Laboratory, 4Navy Technology Center for Safety and Survivability, Chemistry Division, U.S. Naval Research Laboratory


JoVE 51938

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 JoVE Chemistry

Construction of Models for Nondestructive Prediction of Ingredient Contents in Blueberries by Near-infrared Spectroscopy Based on HPLC Measurements

1United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 2Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3Institute of Agriculture, Tokyo University of Agriculture and Technology


JoVE 53981

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 JoVE In-Press

Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

1Department of Medicine, University of Virginia, 2Department of Cell Biology, Neural and Behavioral Science Graduate Program, SUNY Downstate Medical Center, 3Division of Neonatology, University of Virginia, 4Department of Neuroscience and Physiology, New York University School of Medicine, 5Department of Cell Biology, SUNY Downstate Medical Center

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JoVE 55755

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 JoVE Neuroscience

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

1Institute of Biomaterials and Biomedical Engineering, University of Toronto, 2Computational Brain Anatomy Laboratory, Douglas Institute, McGill University, 3McGill Centre for Studies in Aging, McGill University, 4MRI Unit, Research Imaging Centre, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, 5Department of Psychiatry, University of Toronto, 6School of Psychology, University of Wollongong, 7Neuroscience Research Australia, 8Department of Medicine, University of Toronto, 9Kimel Family Translational Imaging Genetics Research Laboratory, Research Imaging Centre, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health


JoVE 51861

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 JoVE Bioengineering

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

1Jülich Centre for Neutron Science Outstation at MLZ, Forschungszentrum Jülich GmbH, 2Department of Chemistry, Louisiana State University, 3Jülich Centre for Neutron Science JCNS-1 & Institute of Complex Systems ICS-1, Forschungszentrum Jülich GmbH, 4Central Institute of Engineering, Electronics and Analytics — Electronic Systems (ZEA-2), Forschungszentrum Jülich GmbH, 5Central Institute of Engineering, Electronics and Analytics — Engineering and Technology (ZEA-1), Forschungszentrum Jülich GmbH


JoVE 54639

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 Science Education: Essentials of Analytical Chemistry

Calibration Curves

JoVE Science Education

Source: Laboratory of Dr. B. Jill Venton - University of Virginia

Calibration curves are used to understand the instrumental response to an analyte and predict the concentration in an unknown sample. Generally, a set of standard samples are made at various concentrations with a range than includes the unknown of interest and the instrumental response at each concentration is recorded. For more accuracy and to understand the error, the response at each concentration can be repeated so an error bar is obtained. The data are then fit with a function so that unknown concentrations can be predicted. Typically the response is linear, however, a curve can be made with other functions as long as the function is known. The calibration curve can be used to calculate the limit of detection and limit of quantitation. When making solutions for a calibration curve, each solution can be made separately. However, that can take a lot of starting material and be time consuming. Another method for making many different concentrations of a solution is to use serial dilutions. With serial dilutions, a concentrated sample is diluted down in a stepwise manner to make lower concentrations. The next sample is made from the previous dilution, and the dilution factor is often kept constant. The advantage is that only one

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 JoVE Bioengineering

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 3Charles Perkins Centre, The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University


JoVE 52975

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 JoVE Immunology and Infection

High-throughput Detection Method for Influenza Virus

1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin


JoVE 3623

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 JoVE Medicine

Shunt Surgery, Right Heart Catheterization, and Vascular Morphometry in a Rat Model for Flow-induced Pulmonary Arterial Hypertension

1Center for Congenital Heart Diseases, Department of Pediatric Cardiology, Beatrix Children's Hospital, University Medical Center Groningen, University of Groningen, 2Research and Development Facility, University Medical Center Groningen, University of Groningen


JoVE 55065

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 JoVE Medicine

Forskolin-induced Swelling in Intestinal Organoids: An In Vitro Assay for Assessing Drug Response in Cystic Fibrosis Patients

1Foundation Hubrecht Organoid Technology, 2Department of Pediatric Pulmonology, Regenerative Medicine Centre Utrecht, Wilhelmina Children's Hospital, University Medical Centre Utrecht, 3Hubrecht Institute for Developmental Biology and Stem Cell Research, University Medical Centre Utrecht


JoVE 55159

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 JoVE Developmental Biology

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

1Immunodynamics, German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics, German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine


JoVE 52544

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 JoVE In-Press

Improving Strength, Power, Muscle Aerobic Capacity, and Glucose Tolerance Through Short-term Progressive Strength Training Among Elderly People

1Åstrand Laboratory of Work Physiology, The Swedish School of Sport and Health Sciences, GIH, 2Department of Neuroscience, Karolinska Institutet, 3Department of Physiology and Pharmacology, Karolinska Institutet

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JoVE 55518

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