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Automation, Laboratory: Controlled operations of analytic or diagnostic processes, or systems by mechanical or electronic devices.
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Live Imaging of Dorsal Root Axons after Rhizotomy

1Temple University, Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, 2Medical Research Service, Department of Veterans Affairs Hospital, 3Department of Neurobiology and Anatomy, Drexel University College of Medicine, 4Shriners Hospitals Pediatric Research Center and Department of Anatomy and Cell Biology, Temple University School of Medicine

JoVE 3126


 Neuroscience

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An Overview of bGDGT Biomarker Analysis for Paleoclimatology

JoVE 10256

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst

Throughout this series of videos, natural samples were extracted and purified in search of organic compounds, called biomarkers, that can relate information on climates and environments of the past. One of the samples analyzed was sediment. Sediments accumulate over geologic time in basins, depressions in the Earth into which sediment flows through the action of fluid (water or air), movement, and gravity. Two main types of basins exist, marine (oceans and seas) and lacustrine (lakes). As one might guess, very different types of life live in these settings, driven in large part by the difference in salinity between them. Over the last few decades, organic geochemists discovered a toolbox of biomarker proxies, or compounds that can be used to describe climate or environment, some of which work in marine environments and some of which work in lacustrine. We turn our attention here to the lacustrine realm and branched glycerol dialkyl glycerol tetraethers (Figure 1). In this section we focus on analysis of terrestrial paleotemperature using branched glycerol dialkyl glycerol tetrathers (Figure 1; brGDGTs) and the MBT/CBT proxy. This proxy was initially described by Weijers et


 Earth Science

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Polymerization

JoVE 10357

Source: Vy M. Dong and Jan Riedel, Department of Chemistry, University of California, Irvine, CA

Polymers are made from macromolecules, which are composed of repeating units (the so called monomeric units). In our modern world, polymers play an important role. One of the first important polymers was nylon, which is a polyamide. It found widespread application in tooth brushes and stockings.


 Organic Chemistry II

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Quantitation of γH2AX Foci in Tissue Samples

1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children's Research Institute, Royal Children's Hospital, 5Department of Pediatrics, The University of Melbourne

JoVE 2063


 Biology

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An Introduction to Reward and Addiction

JoVE 5425

Consequences play a major role in controlling our behavior. If the consequence is a reward, then it encourages the associated behavior. Rewards can come in many forms such as a pleasant feeling, money, or food. However, sometimes an individual engages in compulsive behavior despite of negative consequences, and this state is known as addiction. Administration of addictive substances is neurochemically rewarding, which ultimately causes a loss of control in limiting the intake. Scientists aim to better understand the mechanisms behind these concepts and subsequently develop new therapies for treating substance abuse disorders. JoVE's introduction to reward and addiction explains the neuroanatomical components of the reward pathway. This is followed by some of the important questions asked by behavioral researchers such as how does our brain chemistry change in response to drug use. Prominent methods section reviews some of the tools being employed in the field, like self-administration protocols. Finally, the video discusses example experiments conducted in labs interested in investigating reward and addiction.


 Behavioral Science

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