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Body Fluids: Liquid components of living organisms.

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

1School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, 3School of Public Health, Tianjin Medical University, 4British Columbia Academy, Nanjing Foreign Language School

JoVE 56445


 Chemistry

Generation of Comprehensive Thoracic Oncology Database - Tool for Translational Research

1Pritzker School of Medicine, University of Chicago, 2Department of Medicine, University of Chicago, 3Department of Medicine, Northshore University Health Systems, 4Department of Pathology, University of Chicago, 5Department of Surgery, University of Chicago, 6Department of Biostatistics, University of Chicago

JoVE 2414


 Medicine

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara

JoVE 54102


 Bioengineering

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

1Laboratory of Neurosciences and Circadian Rhythms, School of Medicine, Universidad de los Andes, 2Biophysics Group, Department of Physics, Universidad de los Andes, 3Laboratory of Basic Medical Sciences, School of Medicine, Universidad de los Andes, 4Department of Microbiology and Immunology, University of North Carolina, 5Notre Dame Initiative for Global Development, University of Notre Dame, 6USAID Research and Innovation Fellowship program

JoVE 56238


 Developmental Biology

High-throughput Parallel Sequencing to Measure Fitness of Leptospira interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection

1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine, David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology, David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles

JoVE 56442


 Immunology and Infection

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

1Department of Immunology, Reims University Hospitals, Robert Debré Hospital, 2Faculty of Medicine, LRN EA 4682, University of Reims Champagne-Ardenne, 3Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 4Faculty of Medicine, EA 3797, University of Reims Champagne-Ardenne

JoVE 56012


 Genetics

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre, University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre, University of Nottingham

JoVE 57399


 Immunology and Infection

Using a pH Meter

JoVE 5500

Source: Laboratory of Dr. Zhongqi He - United States Department of Agriculture

Acids and bases are substances capable of donating protons (H+) and hydroxide ions (OH-), respectively. They are two extremes that describe chemicals. Mixing acids and bases can cancel out or neutralize their extreme effects. A substance that is neither acidic nor basic is neutral. The values of proton concentration ([H+]) for most solutions are inconveniently small and difficult to compare so that a more practical quantity, pH, has been introduced. pH was originally defined as the decimal logarithm of the reciprocal of the molar concentration of protons , but was updated to the decimal logarithm of the reciprocal of the hydrogen ion activity . The former definition is now occasionally expressed as p[H]. The difference between p[H] and pH is quite small. It has been stated that pH = p[H] + 0.04. It is common practice to use the term 'pH' for both types of measurements. The pH scale typically ranges from 0 to 14. For a 1 M solution of a strong acid, pH=0 and for a 1 M solution of a strong base, pH=14. Thus, measured pH values will lie mostly in the rang


 General Chemistry

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center

JoVE 52435


 Medicine

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

1Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg

JoVE 55146


 Immunology and Infection

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular, Cinvestav-IPN, 6Subd. de Investigación, Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud

JoVE 51999


 Developmental Biology

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