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Carbonic Anhydrase I: A cytosolic carbonic anhydrase isoenzyme primarily expressed in Erythrocytes, vascular endothelial cells, and the gastrointestinal mucosa. EC 4.2.1.-
 JoVE In-Press

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, IRCCS Centro Cardiologico Monzino

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JoVE 55762

 JoVE Developmental Biology

In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

1Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering, California Institute of Technology


JoVE 54016

 JoVE Medicine

Myo-mechanical Analysis of Isolated Skeletal Muscle

1Cardiovascular Research Institute, University of California San Francisco, 2Department of Pediatrics, University of California San Francisco, 3Department of Biology, San Francisco State University, 4Department of Medicine, University of California San Francisco, 5Eli and Edythe Broad Center of Regeneration Medicine & Stem Cell Research, University of California San Francisco


JoVE 2582

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 JoVE Cancer Research

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

1The Breast Cancer Now Toby Robins Research Centre, Division of Breast Cancer, The Institute of Cancer Research, 2Division of Molecular Pathology, The Institute of Cancer Research, 3Institute of Cancer Sciences, University of Manchester


JoVE 54738

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 JoVE Bioengineering

Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

1Department of Agricultural and Biological Engineering, Birck-Bindley Physiological Sensing Facility, Purdue University, 2NASA Ames Research Center, 3Department of Chemistry, Pennsylvania State University Hazleton, 4Cooley LLP, 5NASA Life and Physical Sciences, Human Exploration and Operations Mission Directorate, NASA Headquarters


JoVE 50020

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 Science Education: Essentials of General Chemistry

Le Châtelier's Principle

JoVE Science Education

Source: Laboratory of Dr. Lynne O'Connell — Boston College

When the conditions of a system at equilibrium are altered, the system responds in such a way as to maintain the equilibrium. In 1888, Henri-Lewis Le Châtelier described this phenomenon in a principle that states, "When a change in temperature, pressure, or concentration disturbs a system in chemical equilibrium, the change will be counteracted by an alteration in the equilibrium composition." This experiment demonstrates Le Châtelier's principle at work in a reversible reaction between iron(III) ion and thiocyanate ion, which produces iron(III) thiocyante ion: Fe3+(aq) + SCN- (aq) FeSCN2+ (aq) The concentration of one of the ions is altered either by directly adding a quantity of one ion to the solution or by selectively removing an ion from the solution through formation of an insoluble salt. Observations of color changes indicate whether the equilibrium has shifted to favor formation of the products or the reactants. In addition, the effect of a temperature change on the solution at equilibrium can be obs

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 Science Education: Essentials of Lab Animal Research

Diagnostic Necropsy and Tissue Harvest

JoVE Science Education

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of the samples. This manuscript will outline proper necropsy techniques for rats.

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 JoVE Bioengineering

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

1Department of Orthopaedic Surgery, University of Michigan Medical School, 2Department of Molecular & Integrative Physiology, University of Michigan Medical School, 3Department of Biomedical Engineering, University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery, University of Michigan Medical School


JoVE 52695

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 JoVE Developmental Biology

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

1Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, 2Department of Molecular Cardiology, Cleveland Clinic Foundation, 3Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University


JoVE 55621

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 JoVE Medicine

A Method for Quantifying Upper Limb Performance in Daily Life Using Accelerometers

1Program in Physical Therapy, Washington University School of Medicine, 2Program in Occupational Therapy, Washington University School of Medicine, 3Department of Neurology, Washington University School of Medicine, 4Mallinckrodt Institute of Radiology, Washington University School of Medicine, 5Department of Biomedical Engineering, Washington University


JoVE 55673

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 JoVE Immunology and Infection

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

1Division of Respirology, Departments of Medicine and Immunology, Toronto Lung Transplant Program, Multiorgan Transplant Program, Toronto General Research Institute, University of Toronto and University Health Network, 2Latner Thoracic Surgery Laboratories, Toronto General Research Institute, University Health Network, 3National Institutes of Health Research, Oxford Biomedical Research Centre, Translational Immunology Laboratory, NDORMS, Kennedy Institute of Rheumatology, University of Oxford, 4Transplantation Research Immunology Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford


JoVE 55283

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