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Cell Degranulation: The process of losing secretory granules (Secretory vesicles). This occurs, for example, in mast cells, basophils, neutrophils, eosinophils, and platelets when secretory products are released from the granules by Exocytosis.

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology, Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy, Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology, Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine, University of Oslo

JoVE 54615


 Immunology and Infection

Investigating Mast Cell Secretory Granules; from Biosynthesis to Exocytosis

1Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, 2Division of Allergy and Immunology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, 3Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences, Tohoku University

JoVE 52505


 Immunology and Infection

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

1Transfusion Research Center, Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences, Ghent University, 3Blood Service, Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care, KULeuven - University of Leuven

JoVE 55351


 Bioengineering

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

1Department of Molecular and Biomedical Sciences, University of Maine, 2Graduate School of Biomedical Sciences and Engineering, University of Maine, 3School of Biology and Ecology, University of Maine, 4Division of Intramural Research, Immunity, Inflammation and Disease Laboratory, National Institute of Environmental Health Sciences, NIH, 5Department of Chemical and Biological Engineering, University of Maine

JoVE 55235


 Immunology and Infection

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins

1Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch National Institute of Dental and Craniofacial Research, National Institutes of Health, 2Department of Biology, University of North Carolina at Chapel Hill, 3Department of Chemical & Biochemical Engineering and Department of Biomedical Engineering, Rutgers University

JoVE 50558


 Biology

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Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus

1Department of Biology, New Mexico State University, 2Department of Molecular Genetics and Microbiology, Duke University, 3Department of Computer Sciences, New Mexico State University, 4Department of Epidemiology of Microbial Diseases, Yale School of Public Health, 5Institute of Applied Biosciences, New Mexico State University

JoVE 55508


 Biology

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Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)

1University of Victoria-Genome BC Proteomics Centre, 2Jewish General Hospital, McGill University, 3Dept of Biochemistry and Microbiology, University of Victoria, 4Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital, McGill University, 5Gerald Bronfman Department of Oncology, Jewish General Hospital

JoVE 55933


 Chemistry

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Method and Instrumented Fixture for Femoral Fracture Testing in a Sideways Fall-on-the-Hip Position

1Department of Physiology and Biomedical Engineering, Mayo Clinic, 2Division of Engineering, Mayo Clinic, 3Department of Orthopedic Surgery, Mayo Clinic, 4Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign

JoVE 54928


 Bioengineering

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Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans

1Centre for Developmental Neurobiology, King's College London, 2Interdisciplinary Bioengineering Graduate Program, Georgia Institute of Technology, 3Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 4School of Chemical & Biomolecular Engineering, Georgia Institute of Technology

JoVE 56292


 Genetics

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Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

1Department of Metabolic Biology, John Innes Centre, Norwich Research Park, 2Department of Botany, University of Wisconsin, Madison, 3Department of Biochemistry and Molecular Biology, Saitama University, 4Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 5Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park

JoVE 56142


 Biology

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Imaging the Root Hair Morphology of Arabidopsis Seedlings in a Two-layer Microfluidic Platform

1Bredesen Center for Interdisciplinary Research and Graduate Education, University of Tennessee, 2Bioscience Division and Center for Nanophase Materials Sciences, Oak Ridge national Laboratory, 3Department of Biochemistry and Cellular and Molecular Biology, University of Tennessee, 4Department of Microbiology, University of Tennessee

JoVE 55971


 Bioengineering

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Rat Model of the Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) Procedure

1Institute of Physiology - Center for Integrative Human Physiology, University of Zurich, 2Department of Surgery, Rush University Medical Center, 3Department of Surgery, Cantonal Hospital Winterthur, 4Institute of Anesthesiology, University and University Hospital Zurich

JoVE 55895


 Medicine

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