Show Advanced Search

REFINE YOUR SEARCH:

Containing Text
- - -
+
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by journal

Filter by science education

 
 
Cell Line: Established cell cultures that have the potential to propagate indefinitely.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.

Microfabrication of Chip-sized Scaffolds for Three-dimensional Cell cultivation

1Institute for Biological Interfaces, Karlsruhe Research Centre, 2Institute for BioMedical Technology, University of Twente, 3Department of Materials Research, Institute for Heavy Ion Research, 4Institute of Microstructure Technology, Karlsruhe Research Centre, 5Institute for Micro Process Engineering, Karlsruhe Research Centre

JoVE 699


 Biology

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.

Introduction to the Microplate Reader

JoVE 5024

The microplate reader is a multimodal instrument that allows for a variety of experiments to be performed and measured simultaneously. Microplate readers can make absorbance, fluorescence and luminescence measurements. Multiwell plates are integral to the microplate reader and allow for many experiments to be performed at once. Regardless of the assay type, experiments on the plate reader utilize a standard curve to determine the experimental values. This curve uses samples of known concentration to generate a line of best fit or standard curve. Experimental values are then extrapolated to the curve or are calculated using the equation from the linear regression. Besides standards and samples being run on the multiwall plate, the blank along with positive and negative controls are also used in the assay to ensure it is working correctly. Multiplate readers are used to quantify protein, gene expression and various metabolic processes such as reactive oxygen species and calcium flux.


 General Laboratory Techniques

Results below contain some, but not all of your search terms.

Understanding Concentration and Measuring Volumes

JoVE 5026

Solutions are utilized to some degree in almost all biological research applications. Therefore understanding how to measure and manipulate them is imperative to any experiment. In this video, concepts in preparing solutions are introduced. Solutions consist of a solute dissolved in solvent to yield a homogeneous mixture of molecular substances. Solutions are generally identified by their components and corresponding concentrations. Concentrated solutions are diluted through various methods, such as serial dilution. This video also lays a foundation for the accurate preparation of solutions. For example, the video reviews how to measure volumes with precision through use of the appropriate volumetric container as well as how to read the volume when a meniscus is present. Some applications for measuring volumes are then presented. Gel electrophoresis is a commonly used laboratory procedure which requires preparation of a percent weight volume solution as well as parallel dilution of a concentrated stock solution. Use of a serial dilution to prepare standards for generating a standard curve in protein quantitation is also demonstrated.


 General Laboratory Techniques

Results below contain some, but not all of your search terms.

An Introduction to the Micropipettor

JoVE 5033

The micropipettor is a common laboratory instrument used for transferring microvolumes of liquid solutions. Micropipettors come in a range of sizes for the accurate movement of volumes between 0.5 and 5000 μl and each instrument requires one of three different sized disposable tips. Micropipettors work by displacing air from the pipette shaft, allowing the liquid to be drawn into the resulting vacuum. Their uses include transferring cell suspensions for a variety of cell-based assays, loading samples for different analytical techniques, and mechanically disrupting tissues into single cell suspensions. Micropipettors are an extremely helpful laboratory tools that are easy to use with a little bit of instruction and practice. In this video, JoVE shows the first-time user all the tips, tricks, and ins and outs of using a micropipettor in the lab.


 General Laboratory Techniques

Results below contain some, but not all of your search terms.

An Introduction to Working in the Hood

JoVE 5036

Hoods are a common class of instrument, which act as a protective enclosure for various types of laboratory experiments. They serve to shield experimental samples from the environment, as well as to protect the researcher from hazardous or infectious samples. “The Hood” is a term that can include laminar flow benches, fume hoods, as well as tissue culture hoods or biosafety cabinets. All hoods operate based on the principle of laminar flow and have the same basic components, including a window sash and protective glass barrier, a non-porous work surface, and an air intake and exhaust. The type of hood used should be chosen based on the experiment. For example, fume hoods typically protect the user, and not the experimental environment. In contrast, laminar flow benches protect the experiment, but not the user, and therefore can but used to keep experimental samples sterile, when they pose no threat. If both the user and the experiment must be protected, a tissue culture hood or biosafety cabinet can be used. Tissue culture hoods maintain sterility of cell lines, while biosafety cabinets provide protection during procedures utilizing infectious agents Once the appropriate unit is chosen for an experiment, the user should wear appropriate protective equipment, and follow all necessary safety procedures. This video discusses the pr


 General Laboratory Techniques

Results below contain some, but not all of your search terms.

Measuring Mass in the Laboratory

JoVE 5037

The analytical balance is the common piece of equipment in the scientific laboratory. These highly precise instruments can measure down to ten thousandths or even hundred thousandths of a gram. The triple beam balance is a type of scale that uses counterweights on a three rider beams to counterbalance the sample in the pan. On the other hand, modern analytical balances use a complex system of electronic sensors to accurately mass a substance. The analytical balance is so sensitive that it often has a draft shield to prevent air currents from interfering with the measurement. When weighing, a weigh boat or weigh paper is used to hold the substance being weighed and protect the weighing pan. Before weighing a substance, analytical balances are tarred to subtract the weigh boat or weigh paper and re-zero the scale. Tarring refers to setting the scale back to read zero and allows for a substance to be accurately weighed. Aside from weighing chemical substances, analytical balances are used to animals or insects, and centrifuge tubes in ultracentrifugation experiments.


 General Laboratory Techniques

Results below contain some, but not all of your search terms.

Introduction to the Spectrophotometer

JoVE 5038

The spectrophotometer is a routinely used instrument in scientific research. Spectrophotometry is the quantitative measurement of how much a chemical substance absorbs light by passing a beam of light through the sample using a spectrophotometer. In this video, basic concepts in spectrophotometry, including transmittance, absorbance and the Beer-Lambert Law are reviewed in addition to the components of the spectrophotometer. These concepts provide a foundation for how to determine the concentration of a solute in solution that is capable of absorbing light in the ultraviolet and visible range. Furthermore, a procedure for how to operate the spectrophotometer is demonstrated, including instructions on how to blank and measure the absorbance of a sample at the desired wavelength. The video also covers how to make a standard curve for determination of analyte concentration. Several applications of the spectrophotometer in biological research are discussed, such as measurement of cell density and determination of chemical reaction rates. Finally, the microvolume spectrophotometer is introduced, as well as its advantage in measuring the quality and quantity of protein and nucleic acids.


 General Laboratory Techniques

Results below contain some, but not all of your search terms.

Introduction to Fluorescence Microscopy

JoVE 5040

Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light wavelengths by a fluorescent molecule known as a fluorophore. Fluorescence microscopy is accomplished in conjunction with the basic light microscope by the addition of a powerful light source, specialized filters, and a means of fluorescently labeling a sample. This video describes the basic principles behind fluorescence microscopy including the mechanism of fluorescence, the Stoke’s shift, and photobleaching. It also gives examples of the numerous ways to fluorescently label a sample including the use of fluorescently tagged antibodies and proteins, nucleic acid fluorescent dyes with, and the addition of naturally fluorescent proteins to a specimen. The major components of the fluorescence microscope including a xenon or mercury light source, light filters, the dichroic mirror, and use of the shutter to illuminate the sample are all described. Finally, examples of some of the many applications for fluorescence microscopy are shown.


 General Laboratory Techniques

1131132133
More Results...