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Circadian Rhythm: The regular recurrence, in cycles of about 24 hours, of biological processes or activities, such as sensitivity to drugs and stimuli, hormone secretion, sleeping, and feeding.

In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells

1Department of Environmental and Occupational Health, School of Public Health, NIEHS Center for Environmental Exposures and Disease, Environmental and Occupational Health Sciences Institute, Rutgers University, 2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency

Video Coming Soon

JoVE 55832


 JoVE In-Press

Drosophila Preparation and Longitudinal Imaging of Heart Function In Vivo Using Optical Coherence Microscopy (OCM)

1Bioengineering Program, Lehigh University, 2Center for Photonics and Nanoelectronics, Lehigh University, 3Department of Electrical and Computer Engineering, Lehigh University, 4State Key Laboratory of Software Engineering, Wuhan University, 5Genetics and Aging Research Unit, Department of Neurology, Massachusetts General Hospital and Harvard Medical School

JoVE 55002


 Biology

Automatic Detection of Highly Organized Theta Oscillations in the Murine EEG

1Department of Psychiatry and Psychotherapy, University of Cologne, 2Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, 3Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases, 4Federal Institute for Drugs and Medical Devices

JoVE 55089


 Behavior

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures

1Department of Genetics, UMR_S 968, Institut de la Vision, 2Department of Visual Information, UMR_S 968, Institut de la Vision, 3Exploratory Team, UMR_S 968, Institut de la Vision, 4Sorbonne Universités, Paris 06, UMR_S 968, Institut de la Vision, 5INSERM, U968, Institut de la Vision, 6CNRS, UMR_7210, Institut de la Vision

JoVE 51954


 Neuroscience

In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

1Institute of Orthopedic Research and Biomechanics, University Medical Center Ulm, 2RISystem, 3Core Facility Small Animal MRI, University Medical Center Ulm, 4Department of Traumatology, Hand-, Plastic-, and Reconstructive Surgery, University Medical Center Ulm

Video Coming Soon

JoVE 56679


 JoVE In-Press

Basic Mouse Care and Maintenance

JoVE 5158

Mice (Mus musculus) are small rodents that breed and sexually mature quickly, making them perfectly suited to generating large animal colonies for biological research. As compared to other mammalian species, mice are simple and inexpensive to maintain in the laboratory. Nevertheless, mouse colonies do have specific husbandry needs that are critical to preserving animal health and safety as well as experimental reproducibility. This video demonstrates standard practices that ensure mice are treated as humanely as possible within the laboratory animal facility, or vivarium. The discussion begins by reviewing a typical mouse housing setup, consisting of a plastic cage equipped with a layer of soft bedding and nesting material. The preformulated food pellets (also known as chow) that comprise the typical mouse diet are also introduced. In order to facilitate experiments performed on mice, safe animal handling practices are demonstrated, including common restraint techniques like “scruffing,” and the strategies used by researchers to keep track of individual mice within the facility. Finally, experimental manipulations of mouse housing and diet are discussed, in addition to one of the most common applications of the scruffing technique — performing injections.


 Biology II

Manipulation of Epileptiform Electrocorticograms (ECoGs) and Sleep in Rats and Mice by Acupuncture

1Department of Sports, Health & Leisure, College of Tourism, Leisure and Sports, Aletheia University, Tainan Campus, 2Department of Neurology, Mackay Memorial Hospital and Mackay Medical College, 3Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, 4Graduate Institute of Brain & Mind Sciences, College of Medicine, National Taiwan University, 5Graduate Institute of Acupuncture Science, College of Chinese Medicine, China Medical University

JoVE 54896


 Behavior

Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells

1INSERM, U968, 2Sorbonne Universités, UPMC Paris 06, UMR_S 968, Institut de la Vision, 3CNRS, UMR_7210, 4Department of Biological Sciences, Center for Cancer, Genetic Diseases and Gene Regulation, Fordham University

JoVE 52100


 Immunology and Infection

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

1Center for Metabolic and Degenerative Diseases, The Brown Foundation Institute of Molecular Medicine, Programs in Human and Molecular Genetics and Neuroscience, The Graduate School of Biomedical Sciences at Houston, The University of Texas Health Science Center at Houston, 2Department of Natural Sciences, University of Houston - Downtown, 3Department of Neurobiology and Anatomy, McGovern Medical School, The University of Texas Health Science Center at Houston

JoVE 55128


 Neuroscience

A Visual Description of the Dissection of the Cerebral Surface Vasculature and Associated Meninges and the Choroid Plexus from Rat Brain

1Division of Neurotoxicology, National Center for Toxicological Research, 2Division of Personalized Nutrition and Medicine, National Center for Toxicological Research, 3Office of Planning, Finance, and Information Technology, National Center for Toxicological Research

JoVE 4285


 Neuroscience

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