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COS Cells: Cell lines derived from the Cv-1 cell line by transformation with a replication origin defective mutant of Sv40 virus, which codes for wild type large T antigen (Antigens, Polyomavirus transforming). They are used for transfection and cloning. (The Cv-1 cell line was derived from the kidney of an adult male African green monkey (Cercopithecus aethiops).)
 JoVE Biology

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London


JoVE 55119

 JoVE Biology

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

1Institut National de la Santé et de la Recherche Médicale, UMR 631, Parc scientifique de Luminy, 2Centre National de la Recherche Scientifique, UMR 6102, Parc scientifique de Luminy, 3Centre d'Immunologie de Marseille-Luminy, Aix-Marseille University, 4École Centrale Marseille, Technopôle de Château-Gombert, 5Institut Fresnel, Aix-Marseille University, 6Centre National de la Recherche Scientifique, UMR 6133, Aix-Marseille University


JoVE 3599

 JoVE Immunology and Infection

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

1Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, 3Department of Pathology, UT Health Science Center at San Antonio, 4Department of Microbiology, UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center, UT Health Science Center at San Antonio


JoVE 50752

 JoVE Medicine

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

1Tumor Microenvironment and Metastasis Program, Wistar Institute, 2Department of Pathology and Lab Medicine, Perelman School of Medicine, University of Pennsylvania, 3Department of Microbiology and Immunology and Department of Genetics, Geisel School of Medicine at Dartmouth, 4Division of Endocrine and Oncologic Surgery, Department of Surgery, Perelman School of Medicine, University of Pennsylvania, 5Rena Rowan Breast Center, Abramson Cancer Center, University of Pennsylvania, 6Center for Advanced Medicine, University of Pennsylvania


JoVE 51171

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 Science Education: Essentials of Physics I

Kinematics and Projectile Motion

JoVE Science Education

Source: Ketron Mitchell-Wynne, PhD, Asantha Cooray, PhD, Department of Physics & Astronomy, School of Physical Sciences, University of California, Irvine, CA

This experiment demonstrates the kinematics of motion in 1 and 2 dimensions. This lab will begin by studying motion in 1 dimension, under constant acceleration, by launching a projectile directly upward and measuring the maximum height reached. This lab will verify that the maximum height reached is consistent with the kinematic equations derived below. Motion in 2 dimensions will be demonstrated by launching the ball at an angle θ. Using the kinematic equations below, one can predict the distance to where the projectile will land based upon the initial speed, total time, and angle of trajectory. This will demonstrate kinematic motion with and with out acceleration in the y- and x-directions, respectively.

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 JoVE Medicine

Coordinate Mapping of Hyolaryngeal Mechanics in Swallowing

1Medical College of Georgia, Georgia Regents University, 2Department of Communicative Sciences and Disorders, New York University, 3Department of Cellular Biology & Anatomy, Georgia Regents University, 4Department of Otolaryngology, Georgia Regents University


JoVE 51476

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 Science Education: Essentials of Physics II

Interference and Diffraction

JoVE Science Education

Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN

Interference and diffraction are characteristic phenomena of waves, ranging from water waves to electromagnetic waves such as light. Interference refers to the phenomenon of when two waves of the same kind overlap to give an alternating spatial variation of large and small wave amplitude. Diffraction refers to the phenomenon of when a wave passes through an aperture or goes around an object, different parts of the wave can interfere and also give rise to a spatial alternation of large and small amplitude. This experiment will demonstrate the wave nature of the light by observing diffraction and interference of a laser light passing through a single slit and double slits, respectively. The slits are simply cut using razor blades in an aluminum foil and the characteristic diffraction and interference patterns manifest as patterns of alternating light and dark fringes on a screen placed after the foil, when the light is shone through the slit(s) on the foil. Historically, the observation of diffraction and interference of light played important roles in establishing that light is an electromagnetic wave.

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