JoVE Developmental Biology
Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
1InvivoSciences, Inc., 2Gilson, Inc.
Culture:
A collective expression for all behavior patterns acquired and socially transmitted through symbols. Culture includes customs, traditions, and language.
JoVE Neuroscience
Inhibitory Synapse Formation in a Co-culture Model Incorporating GABAergic Medium Spiny Neurons and HEK293 Cells Stably Expressing GABAA Receptors
1UCL School of Pharmacy, University College London
JoVE Developmental Biology
An Optogenetic Approach for Assessing Formation of Neuronal Connections in a Co-culture System
1Neuroscience & Behavioral Disorders, Duke-NUS Graduate Medical School, 2Lee Kong Chian School of Medicine, Nanyang Technological University
JoVE Biology
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
1Neuroscience Division of the Biomedical & Health Sciences Institute, University of Georgia, 2Department of Cellular Biology, University of Georgia
JoVE Developmental Biology
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
1Centre for Integrative Physiology, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh
JoVE Bioengineering
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
1Department of Chemical Engineering and Centre for Regenerative Medicine, University of Bath, 2MRC Centre for Drug Safety Science and Institute of Translational Medicine, University of Liverpool, 3Mechanical Engineering, University College London, 4Department of Applied Mathematics, Liverpool John Moores University
JoVE Biology
Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact
1Dept. of Medical Biology, University of Québec
JoVE Biology
Isolation and Culture of Neonatal Mouse Cardiomyocytes
1Randall and Cardiovascular Divisions, King’s College London, 2Division of Cardiology, School of Medicine, University of California San Diego
JoVE Biology
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
1Fachbereich Biologie, Entwicklungsbiologie, Philipps-Universität Marburg, 2Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg
JoVE In-Press
The Aortic Ring Co-culture Assay: A Convenient Tool to Assess the Angiogenic Potential of Mesenchymal Stromal Cells In Vitro
1Create Fertility Centre, 2Department of Physiology, University of Toronto, 3Department of Obstetrics and Gynecology, University of Toronto, 4Department of Medical Sciences, University of Toronto, 5Department of Obstetrics and Gynecology, Women's College Hospital
JoVE Medicine
A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies
1Department of Cancer Biology, Dana-Farber Cancer Institute, 2Department of Pediatrics, Children's Hospital, 3Department of Biostatistics & Computational Biology, Dana-Farber Cancer Institute, 4Department of Developmental Biology and Cancer Research, Hebrew University of Jerusalem, 5Department of Neurosurgery, Children's Hospital, 6Center for Molecular Oncologic Pathology, Department of Medical Oncology, Dana-Farber Cancer Institute
JoVE Immunology and Infection
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
1Department of Pathology, UT Southwestern Medical Center
JoVE Biology
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
1School of Biosciences, University of Birmingham
JoVE Environment
A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry
1Department of Biological Sciences, University of Alberta
JoVE Developmental Biology
Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans
1Institute of Ageing and Chronic Disease, University of Liverpool, 2Aintree University Hospital
JoVE Bioengineering
Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
1Biomedical Engineering Graduate Program, The University of Western Ontario, 2Department of Anatomy & Cell Biology, Schulich School of Medicine & Dentistry, The University of Western Ontario, 3Department of Chemical Engineering, Queen's University, 4Department of Chemical & Biochemical Engineering, Faculty of Engineering, The University of Western Ontario
JoVE Immunology and Infection
Preparation of a Blood Culture Pellet for Rapid Bacterial Identification and Antibiotic Susceptibility Testing
1Institute of Microbiology, University Hospital Center and University of Lausanne
JoVE Neuroscience
A Simplified Method for Ultra-Low Density, Long-Term Primary Hippocampal Neuron Culture
1Department of Basic Medical Sciences, University of Arizona College of Medicine-Phoenix, 2Jiangsu Provincial Center for Disease Control and Prevention
JoVE Biology
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres
1Center for Applied Proteomics and Molecular Medicine, George Mason University, 2Virginia Surgery Associates
JoVE Biology
Isolation, Culture and Transduction of Adult Mouse Cardiomyocytes
1Cardiovascular Research Institute, University of California, San Francisco
JoVE Medicine
Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity
1Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, University of Chicago
JoVE Bioengineering
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
1Department of Biomedical Engineering, The University of Texas at Austin, 2Wyss Institute for Biologically Inspired Engineering at Harvard University, 3Vascular Biology Program, Boston Children's Hospital, Harvard Medical School, 4John A. Paulson School of Engineering and Applied Sciences, Harvard University
JoVE Neuroscience
Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
JoVE In-Press
Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
1Center for Peptide Pharmaceuticals, Faculty of Natural Science, Ulm University
JoVE Biology
Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
1Department of Physiology and Biophysics, University of Colorado Anschutz Medical Campus, 2Department of Bioengineering, University of Colorado Anschutz Medical Campus, 3Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus
JoVE Immunology and Infection
Propagation of Homalodisca coagulata virus-01 via Homalodisca vitripennis Cell Culture
1Department of Biology, University of Texas at Tyler, 2U. S. Horticultural Research Laboratory, USDA ARS
JoVE Bioengineering
Self-reporting Scaffolds for 3-Dimensional Cell Culture
1School of Molecular Medical Sciences, University of Nottingham, 2Division of Drug Delivery and Tissue Engineering, University of Nottingham, 3Laboratory of Biophysics and Surface Analysis, University of Nottingham
JoVE Bioengineering
A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
1Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Taiwan, 2Tissue Engineering and Regenerative Medicine, National Chung Hsing University, 3Institute of NanoEngineering and MicroSystems, National Tsing Hua University
JoVE Developmental Biology
Epicardial Outgrowth Culture Assay and Ex Vivo Assessment of Epicardial-derived Cell Migration
1Aab Cardiovascular Research Institute, University of Rochester School of Medicine and Dentistry, 2Department of Medicine, University of Rochester School of Medicine and Dentistry, 3Department of Pharmacology and Physiology, University of Rochester School of Medicine and Dentistry
JoVE Medicine
A Three-dimensional Tissue Culture Model to Study Primary Human Bone Marrow and its Malignancies
1Department of Biological Sciences, Purdue University, 2Department of Oncology, University of Alberta, 3Cross Cancer Institute
JoVE Neuroscience
Dissection, Culture, and Analysis of Xenopus laevis Embryonic Retinal Tissue
1Department of Biology, College of William and Mary
JoVE Biology
Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
1Cell and Developmental Biology, University of Michigan, 2Department of Biosciences and Nutrition, Karolinska Instituet Novum
JoVE Neuroscience
Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays
1Department of Otorhinolaryngology, Inselspital and University of Bern, 2Department of Clinical Research, Inselspital and University of Bern, 3Department of Physiology, University of Bern, 4Department of Clinical Neurosciences, Service ORL & HNS, University Hospital Geneva
JoVE In-Press
Initiation and Maintenance of a Rat Brain Cell Aggregate Culture: A Suitable In Vitro Model for Primary Blast-induced Brain Injury
1Defence Research & Development Canada
JoVE Bioengineering
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
1Department of Chemical and Biomolecular Engineering, University of Delaware, 2Department of Materials Science and Engineering, University of Delaware
JoVE Developmental Biology
Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption
1INRS-Institut Armand-Frappier
JoVE Developmental Biology
An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
1Dept. of Biomedicine, Pharmacenter, University of Basel, 2Molecular Signalling and Gene Therapy, Narayana Nethralaya Foundation, Narayana Health City, 3Brain Ischemia and Regeneration, Department of Biomedicine, University Hospital Basel, 4Department of Neurosurgery, Klinikum Idar-Oberstein, 5Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 6Department of Neurology, Laboratory of Molecular Neuro Oncology, University Hospital of Zurich
JoVE Immunology and Infection
Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
1Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago
JoVE Biology
Modified ES / OP9 Co-Culture Protocol Provides Enhanced Characterization of Hematopoietic Progeny
1Department of Medicine, Hematology-Oncology, University of California, Los Angeles, 2Department of Biological Chemistry, University of California, Los Angeles
JoVE Neuroscience
Isolation and Culture of Adult Neural Stem Cells from the Mouse Subcallosal Zone
1Department of Anatomy, Korea University College of Medicine
JoVE Medicine
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry
1Department of Neurosurgery, Henry Ford Hospital
JoVE Bioengineering
Cell Co-culture Patterning Using Aqueous Two-phase Systems
1Department of Biomedical Engineering, University of Michigan, 2Department of Macromolecular Science and Engineering, University of Michigan
JoVE Developmental Biology
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
1Developmental Biology, The Roslin Institute and R(D)SVS, The University of Edinburgh
JoVE Neuroscience
Low-Density Primary Hippocampal Neuron Culture
1Department of Physiology and Pathophysiology, University of Manitoba, 2Kleysen Institute for Advanced Medicine, Health Sciences Centre
JoVE Bioengineering
Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
1Dept. of Clinical and Experimental Medicine, University of Pisa, 2OtoLab, Azienda Ospedaliero-Universitaria Pisana (AOUP), 3Dept. of Civil and Industrial Engineering, University of Pisa, 4Immunohematology Operative Unit, Azienda Ospedaliero-Universitaria Pisana (AOUP), 5Dept. Of Surgical, Medical, Molecular Pathology and Emergency Medicine, University of Pisa, 6II Orthopedic and Traumatologic Clinic, Azienda Ospedaliero-Universitaria Pisana (AOUP)
JoVE Medicine
Primary Culture of Human Vestibular Schwannomas
1Department of Otolaryngology-Head and Neck, University of Iowa Hospitals and Clinics
JoVE Immunology and Infection
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
1Department of Biomedical Sciences and Pathobiology, Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering, Cornell University, 3School of Electrical and Computer Engineering, Cornell University, 4Department of Biomedical Engineering, Duke University
JoVE Biology
Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
1INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 2CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Université Lyon 1, 5Centre Léon Bérard
JoVE In-Press
Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
1The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, 2Institute of Chemical Materials, China Academy of Engineering Physics
JoVE Neuroscience
Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration
1Centre for Neuroprosthesis, EPFL | STI | IMT/IBI | LSBI, 2Blond McIndoe Research Laboratories, Institute of Inflammation & Repair, The University of Manchester, 3University Hospital of South Manchester
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