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Cystic Fibrosis: An autosomal recessive genetic disease of the Exocrine glands. It is caused by mutations in the gene encoding the Cystic fibrosis transmembrane conductance regulator expressed in several organs including the Lung, the Pancreas, the Biliary system, and the Sweat glands. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in Airway obstruction; chronic Respiratory infections; Pancreatic insufficiency; maldigestion; salt depletion; and Heat prostration.

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

1Wellness Promotion Science Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 2Advanced Research Center for Human Sciences, Waseda University, 3Department of Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, 4Asanogawa General Hospital

JoVE 54837


 Medicine

Phenotyping Mouse Pulmonary Function In Vivo with the Lung Diffusing Capacity

1Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health, 2Department of International Health, Johns Hopkins University Bloomberg School of Public Health, 3Department of Medicine, Johns Hopkins University School of Medicine

JoVE 52216


 Biology

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

1Center for Environmental Medicine, Asthma, and Lung Biology, The University of North Carolina at Chapel Hill, 2Department of Pediatrics, The University of North Carolina at Chapel Hill, 3Pulmonary Diseases and Critical Care, The University of North Carolina at Chapel Hill, 4Curriculum in Toxicology, The University of North Carolina at Chapel Hill

JoVE 50646


 Biology

Evolutionary Relationships- Concept

JoVE 10561

Humans have been attempting to properly classify living things since Aristotle made the first attempt during the 4th century BC. Aristotle’s system was improved upon during the Renaissance and then, subsequently, by Carolus Linnaeus in the mid 1700’s. These more formal classification and organization systems grouped species by their physical similarity to one another. For example,…


 Lab Bio

Preparing and Administering Inhaled Medications

JoVE 10390

Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT


Inhaled medications are prescribed for conditions affecting the bronchi, which branch off of the trachea, and bronchioles, which are progressively smaller conducting airways spread throughout the lung tissue. These conditions can be…


 Nursing Skills

Respiratory Exam I: Inspection and Palpation

JoVE 10028

Source: Suneel Dhand, MD, Attending Physician, Internal Medicine, Beth Israel Deaconess Medical Center


Disorders of the respiratory system with a chief complaint of shortness of breath are among the most common reasons for both outpatient and inpatient evaluation. The most obvious visible clue to a respiratory problem will be whether the…


 Physical Examinations I

Chromatography-based Biomolecule Purification Methods

JoVE 5683

In biochemistry, chromatography-based purification methods are employed to isolate compounds from a complex mixture. Two such methods used commonly by biochemists are size-exclusion chromatography and affinity chromatography. In size-exclusion chromatography, a column packed with porous beads separates components of a mixture based on size. On the other hand, affinity chromatography allows for …


 Biochemistry

SNP Genotyping

JoVE 5544

Single nucleotide polymorphisms, or SNPs, are the most common form of genetic variation in humans. These differences at individual bases in the DNA often do not directly affect gene expression, but in many cases can still be useful for locating disease-associated genes or for diagnosing patients. Numerous methodologies have been established to identify, or…


 Genetics

An Introduction to Saccharomyces cerevisiae

JoVE 5081

Saccharomyces cerevisiae (commonly known as baker’s yeast) is a single-celled eukaryote that is frequently used in scientific research. S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. Because many yeast proteins are similar in sequence and function…


 Biology I

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva

1Department of Medicine, Weill Cornell Medical College, 2Department of Oral Biology, University of Missouri-Kansas City-School of Dentistry, 3Department of Pharmacology and Toxicology, University of Missouri Kansas City- School of Pharmacy, 4Regional Hospital, Bamenda, NWP, Cameroon, 5Mezam Polyclinic HIV/AIDS Treatment Center, Cameroon, 6Institute for Human Genetics and Biochemistry

JoVE 3999


 Medicine

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