Show Advanced Search

REFINE YOUR SEARCH:

Containing Text
- - -
+
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by journal section

Filter by science education

 
 
Dendritic Cells: Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as Skin and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process Antigens, and present them to T-Cells, thereby stimulating Cell-mediated immunity. They are different from the non-hematopoietic Follicular dendritic cells, which have a similar morphology and immune system function, but with respect to humoral immunity (Antibody production).

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

JoVE 50951

 Immunology and Infection

Cell-mediated Immune Responses

JoVE 10896

The cell-mediated immune system is the host’s primary response against invasive bacteria and viruses that cause intracellular infections. It is also essential for fighting against and destroying cancer cells. Furthermore, the cell-mediated immune system plays a role in the rejection of organ transplants or graft tissue.

Phagocytic cells of the innate immune system, such as macrophages or dendritic cells, are the first to recognize a foreign particle. These cells engulf the foreign particle and digest it. Small molecules of the foreign particle, called antigens, remain intact and are presented at the surface of the phagocytic cell. The presentation is facilitated by proteins of the major histocompatibility complex (MHC), which binds the antigen and protrude from the cell. The phagocytic cell is therefore also called an antigen presenting cell (APC). The MHC-antigen complex activates cells of the adaptive immune system, which eventually fight the source of the foreign particle. T cells are a type of lymphocyte that are named after their location of maturation—the thymus. In the thymus, precursor T cells differentiate into two main types, CD4+ and CD8+ T cells. These cell types are named after the surface receptor that determines the cell’s function. All T cells carry T-cell receptors, but the coreceptor CD4

 Core: Immune System

Inflammation

JoVE 10902

In response to tissue injury and infection, mast cells initiate inflammation. Mast cells release chemicals that increase the permeability of adjacent blood capillaries and attract additional immune cells to the wound or site of infection. Neutrophils are phagocytic leukocytes that exit the bloodstream and engulf invading microbes. Blood clotting platelets seal the wound and fibers create a scaffold for wound healing. Macrophages engulf aging neutrophils to end the acute inflammatory response. Tissue injury and infection are the primary causes of acute inflammation. Inflammation protects the body by eliminating the cause of tissue injury and initiating the removal of cell debris resulting from the initial damage and related immune cell activity. Inflammation involves mediators of both the innate and adaptive immune system. Proper regulation of inflammation is crucial to clear the pathogen and remove cell debris without overly damaging healthy tissue in the process. If inflammatory processes are not properly regulated, chronic inflammation can arise that is often fatal. Mast cells are the first to respond to tissue injury, as they are primarily located in areas that have contact with the exterior: the skin, gut, and airways. Mast cells have an arsenal of receptors on their cell surface and can hence be activated by a wide variety of stimuli, such as mi

 Core: Immune System

Vaccinations

JoVE 10903

Vaccination is the administration of antigenic material from pathogens to confer immunity against a specific microorganism. Vaccination primes the immune system to recognize and mount an immune response faster and more effectively if the real pathogen is encountered. Vaccinations are one of the most efficient ways to protect both individual humans and the general public from disease. A growing anti-vaccination skepticism risks the successes of vaccination programs that helped reduce and, in some instances, eradicate fatal diseases. Vaccines can be administered via oral and intranasal routes, as well as by injection into the muscle (intramuscular), the fat layer under the skin (subcutaneous), or the skin (intradermal). Vaccines contain antigens derived from a specific pathogen. Those containing “dead” antigens, which are intact but unable to replicate, are referred to as inactive vaccines. By contrast, subunit vaccines contain only parts of the pathogen. Some vaccines contain the live pathogen in a weakened (attenuated) form. An attenuated pathogen stimulates the immune system without causing severe disease. Vaccines often contain adjuvants, chemicals that enhance the immune response against the pathogen. When a vaccine is administered, antigen-presenting immune cells (APCs), such as dendritic cells or macrophages, engulf the antigen from the v

 Core: Immune System

Allergic Reactions

JoVE 10901

We speak of an allergy when the immune system triggers a response against a benign foreign structure, like food, pollen or pet dander. These elicitors are called allergens. If the immune system of a hypersensitive individual was primed against a specific allergen, it will trigger allergic symptoms during every subsequent encounter of the allergen. Symptoms can be mild, such as hay fever, to severe, such as potentially fatal anaphylactic shock. The immune system is crucial for defending an organism against bacteria, viruses, fungi, toxins, and parasites. However, in a hypersensitive response, it can be triggered by harmless substances and cause unpleasant or potentially life-threatening overreactions, called allergies. The first step toward establishing an allergy is sensitization. For instance, an individual becomes allergic to the pollen of ragweed when, for the first time, immune cells in the respiratory passage take up the pollen and degrade the allergens into fragments. These immune cells are called antigen-presenting cells, or APCs, because they display the degraded allergen fragments on their surface. Examples of APCs are dendritic cells, macrophages and B cells. Subsequently, APCs activate encountered Type 2 helper T cells (Th2). The activated Th2 then release chemical signals (e.g., cytokines) that cause B cells to differen

 Core: Immune System

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

1LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 3CIBER de Enfermedades Cardiovasculares

JoVE 58118

 Immunology and Infection

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

1Department of Dermatology, Yale University School of Medicine, 2Dermatology Department, University of Rome Tor Vergata, 3Department of Pathology, Yale University School of Medicine

JoVE 59370

 Immunology and Infection

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

1Barbara Davis Center for Childhood Diabetes, University of Colorado Denver, 2Department of Medicine, Endocrinology, Diabetes & Metabolism, Baylor College of Medicine, 3Scientific Center, Shandong Provincial Hospital affiliated to Shandong University

JoVE 59985

 Immunology and Infection

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis

1School of Life Science and Technology, ShanghaiTech University, 2Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 3University of Chinese Academy of Sciences, 4Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University

JoVE 55700

 Developmental Biology

Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

JoVE 59897

 Genetics

Automated Quantification of Hematopoietic Cell – Stromal Cell Interactions in Histological Images of Undecalcified Bone

1Immunodynamics, German Rheumatism Research Center, a Leibniz Institute, 2Biophysical Analytics, German Rheumatism Research Center, a Leibniz Institute, 3Max-Delbrück Center for Molecular Medicine, 4Wimasis GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine

JoVE 52544

 Developmental Biology

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

1Division of Respirology, Departments of Medicine and Immunology, Toronto Lung Transplant Program, Multiorgan Transplant Program, Toronto General Research Institute, University of Toronto and University Health Network, 2Latner Thoracic Surgery Laboratories, Toronto General Research Institute, University Health Network, 3National Institutes of Health Research, Oxford Biomedical Research Centre, Translational Immunology Laboratory, NDORMS, Kennedy Institute of Rheumatology, University of Oxford, 4Transplantation Research Immunology Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford

JoVE 55283

 Immunology and Infection

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine

JoVE 56364

 Bioengineering

Bone Marrow Transplantation Platform to Investigate the Role of Dendritic Cells in Graft-versus-Host Disease

1Cancer Division, Burnett School of Biomedical Sciences, University of Central Florida, 2Department of Biochemistry, Hanoi University of Pharmacy, 3Center for Cardiovascular Regeneration, Department of Cardiovascular Sciences, Houston Methodist Research Institute, 4Department of Epidemiology, University of Pittsburgh Graduate School of Public Health, 5Division of Cancer Control and Population Sciences, University of Pittsburgh Medical Center, Hillman Cancer Center

Video Coming Soon

JoVE 60083

 JoVE In-Press

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

1Department of Microbiology and Parasitology, College of Basic Medical Sciences, China Medical University, 2Department of Obstetrics and Gynecology, College of Medicine, University of Arkansas for Medical Sciences, 3Department of Pathology, College of Medicine, University of Arkansas for Medical Sciences

JoVE 3657

 Immunology and Infection

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

1Department of Biology, University of Mary Hardin-Baylor, 2Department of Pediatrics, University of Texas Medical Branch, 3Multi-Organ Support Technology Task Area, U.S. Army Institute of Surgical Research, 4Internal Medicine, Texas A&M University Health Science Center

JoVE 55126

 Developmental Biology

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-photon Intravital Microscopy

1Faculty of Biomedical Sciences, Institute for Research in Biomedicine, Università della Svizzera italiana (USI), 2Graduate School of Cellular and Molecular Sciences, Faculty of Medicine, University of Bern, 3Institute of Computational Science, Università della Svizzera italiana (USI)

JoVE 58355

 Immunology and Infection

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

1Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University

JoVE 55266

 Immunology and Infection

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

1Biophysical Analytics, German Rheumatism Research Center, Leibniz Institute, 2Microscopy Core Facility, Max-Delbrück Center for Molecular Medicine, 3Immunodynamics, German Rheumatism Research Center, Leibniz Institute, 4LaVision Biotec GmbH, 5Immunodynamics and Intravital Imaging, Charité - University of Medicine

JoVE 51135

 Immunology and Infection
123457
More Results...