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Dimethyl Sulfoxide: A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during Cryopreservation. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.

Cell Division - Prep Student

JoVE 10626

Preparation of Onion Root Tips and Solutions
For the cell cycle experiment using root tips, first, leave an onion suspended over a beaker of water to grow roots for several days.
Then, clean the onion roots of any dirt or debris.
Next, in a 1.5 mL tube, dissolve 10 mcg of nocodazole per 1 mL of dimethyl sulfoxide solution, making 1 tube…

 Lab Bio

Ozonolysis of Alkenes

JoVE 10339

Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA


This experiment will demonstrate an example of an ozonolysis reaction to synthesize vanillin from isoeugenol (Figure 1). Ozonolysis of alkenes, an oxidation reaction between ozone and an alkene, is a common method to prepare…

 Organic Chemistry II

Internal Standards

JoVE 10225

Source: Laboratory of Dr. B. Jill Venton - University of Virginia



The goal of many chemical analyses is a quantitative analysis, where the amount of a substance in a sample is determined. In order to accurately calculate the concentration of an unknown from a sample, careful sample preparation is key. Every time a sample is…

 Analytical Chemistry

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

1Hepatic Fibrosis Group, QIMR Berghofer Medical Research Institute, 2School of Pharmacy and Biomedical Sciences, Curtin Health Innovation Research Institute, Curtin University, 3School of Veterinary and Life Sciences, Murdoch University, 4School of Medicine, The University of Queensland, 5Madrid Institute for Advanced Studies (IMDEA) in Food, CEI UAM+CSIC, 6School of Medicine, Griffith University, 7School of Biological Sciences, Queen's University Belfast, 8Department of Gastroenterology & Hepatology, Fiona Stanley and Fremantle Hospital Group, 9School of Medical and Health Sciences, Edith Cowan University, 10Centre for Cell Therapy and Regenerative Medicine, School of Biomedical Sciences, University of Western Australia

Video Coming Soon

JoVE 60992

 JoVE In-Press

Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ

1Ramón y Cajal Institute for Health Research (IRYCIS), Ramón y Cajal University Hospital, 2Centro Integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O'Higgins, 3Department of Molecular Biology, Faculty of Sciences, Universidad Autónoma de Madrid (UAM), 4Instituto de Investigaciones Biosanitarias, Facultad de Ciencias Experimentales, Universidad Francisco de Vitoria, 5Department of Radiology, Rehabilitation & Physiotherapy, Faculty of Medicine, Complutense University

Video Coming Soon

JoVE 60859

 JoVE In-Press

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Department of Medicine, Division of Cardiovascular Medicine, Stanford University, 3Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 4Department of Medicine III, University Hospital Heidelberg

Video Coming Soon

JoVE 60888

 JoVE In-Press

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

Video Coming Soon

JoVE 60872

 JoVE In-Press

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

1Institute of Molecular Virology, Ulm University Medical Center, 2CNRS UMR-8601, Centre Interdisciplinaire Chimie Biologie, 3Team Chemistry & Biology, Modeling & Immunology for Therapy, Université Paris Descartes, 4Université Paris Descartes, Sorbonne Paris Cité, 5Pediatric Otolaryngology Department, Hôpital Necker-Enfants Malades, Assistance Publique Hôpitaux de Paris, 6Université de Paris, Institut Cochin

Video Coming Soon

JoVE 60914

 JoVE In-Press

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2The Michael J. Fox Foundation for Parkinson's Research, 3Department of Medical and Molecular Genetics, Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee

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JoVE 58956

 JoVE In-Press

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors

1Research Program in Molecular and Integrative Biosciences, University of Helsinki, 2Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, 3School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds

JoVE 60619

 Biochemistry

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

1Department of Chemistry, Adelphi University, 2Department of Chemistry, Washington University in St. Louis, 3Department of Chemistry, Boston University

JoVE 59928

 Chemistry

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

1Department of Molecular Biosciences, University of Texas at Austin, 2School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, 3Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 4Department of Microbiology and Immunology, Dalhousie University, 5Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University

JoVE 59920

 Immunology and Infection
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