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DNA, Satellite: Highly repetitive DNA sequences found in Heterochromatin, mainly near centromeres. They are composed of simple sequences (very short) (see Minisatellite repeats) repeated in tandem many times to form large blocks of sequence. Additionally, following the accumulation of mutations, these blocks of repeats have been repeated in tandem themselves. The degree of repetition is on the order of 1000 to 10 million at each locus. Loci are few, usually one or two per chromosome. They were called satellites since in density gradients, they often sediment as distinct, satellite bands separate from the bulk of genomic DNA owing to a distinct Base composition.
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 JoVE Developmental Biology

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

1Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 2Department of Biological Structure, University of Washington School of Medicine, 3Department of Biochemistry, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center


JoVE 52802

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 JoVE In-Press

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

1Department of Medicine, Institute of Translational Pharmacology, National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Department of Life Sciences, University of Modena and Reggio Emilia, 4Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, KAUST

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JoVE 55908

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 JoVE Environment

Integrating Remote Sensing with Species Distribution Models; Mapping Tamarisk Invasions Using the Software for Assisted Habitat Modeling (SAHM)

1Natural Resource Ecology Laboratory, Colorado State University, 2U.S. Geological Survey, Fort Collins Science Center, 3U.S. Geological Survey - U.S. Department of the Interior, North Central Climate Science Center


JoVE 54578

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 JoVE In-Press

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

1CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

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JoVE 55743

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 JoVE Biology

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

1Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh, 2Department of Orthopedic Surgery, University of Pittsburgh, 3Nazarbayev University Research and Innovation System, Nazarbayev University, 4Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 5Department of Cell Biology, Erasmus MC Stem Cell Institute, 6OHSU Center for Regenerative Medicine, Oregon Health & Science University, 7Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh, 8David Geffen School of Medicine and the Orthopaedic Hospital Research Center, University of California at Los Angeles, 9Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh


JoVE 51195

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 JoVE Medicine

Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling

1Department of Cell Biology, UT Southwestern Medical Center, 2National Institute of Neurological Disorders and Stroke, National Institute of Health, 3Division of Pediatric Pathology, Department of Pathology and Laboratory Medicine, Medical College of Wisconsin, 4Division of Genetics and Genomics, Boston Children's Hospital


JoVE 52307

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 JoVE Genetics

Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System

1Department of Occupational and Environmental Health, University of Arkansas for Medical Sciences, 2Department of Environmental Health Sciences, University of Alabama at Birmingham, 3Division of Radiation Health, University of Arkansas for Medical Sciences


JoVE 54969

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 Science Education: Essentials of Developmental Biology

Tissue Regeneration with Somatic Stem Cells

JoVE Science Education

Somatic or adult stem cells, like embryonic stem cells, are capable of self-renewal but demonstrate a restricted differentiation potential. Nonetheless, these cells are crucial to homeostatic processes and play an important role in tissue repair. By studying and manipulating this cell population, scientist may be able to develop new regenerative therapies for injuries and diseases.

This video first defines somatic stem cells, and then explores the role these cells play in tissue regeneration. This is emphasized in a description of a protocol that isolates muscle satellite cells and uses them to repair muscle damage in a mouse model of muscular dystrophy. Finally, we discuss specific tissue regeneration studies utilizing somatic stem cells.

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 Science Education: Essentials of Environmental Science

Using GIS to Investigate Urban Forestry

JoVE Science Education

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University

Urban forests broadly include urban parks, street trees, landscaped boulevards, public gardens, river and coastal promenades, greenways, river corridors, wetlands, nature preserves, natural areas, shelterbelts of trees, and working trees at industrial brownfield sites. The history of urban trees begins with trees as landscape embellishment. Today, urban trees are seen as essential components of city infrastructure and critical to human life as food, housing, and other public utilities. Urban trees are now valued for the ecosystem services they provide (e.g., preventing erosion, air pollutant removal, oxygen, shade, etc.). Yet, to efficiently make use of these benefits, trees must reach maturity, as leaf number and size directly affect a tree’s ability to provide ecosystem services. Urban forestry has had to develop its own forestry methods to address the needs and challenges unique to urban trees as compared to their woodland counterparts. The following excerpt from the USDA Forest Service illustrates the urban tree perspective and policies of federal government: Urban forests are dynamic ecosystems that provide needed environmental services by cleanin

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 JoVE Immunology and Infection

Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field

1USGS Western Ecological Research Center, 2Wildlife Health Center, University of California, Davis, 3Department of Population Health and Reproduction, University of California, Davis, 4Department of Veterinary and Biomedical Sciences, University of Minnesota, 5Science Applications International Corporation


JoVE 2829

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 JoVE Immunology and Infection

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing

1School of Molecular Bioscience, University of Sydney, 2Department of Surgery, Royal Prince Alfred Hospital, 3Department of Anatomical Pathology, Department of Anatomical Pathology, 4Department of Medicine, Concord Repatriation General Hospital


JoVE 3322

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