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Endothelial Cells: Highly specialized Epithelial cells that line the Heart; Blood vessels; and lymph vessels, forming the Endothelium. They are polygonal in shape and joined together by Tight junctions. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

1Developmental Biology, The Roslin Institute and R(D)SVS, University of Edinburgh, 2Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, 3Clinical Sciences and R(D)SVS, University of Edinburgh

JoVE 56126


 Developmental Biology

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance

1BIOS Lab on a Chip group, MIRA Institute for Biomedical Technology and Technical Medicine, MESA+ Institute for Nanotechnology and Max Planck Center for Complex Fluid Dynamics, University of Twente, 2Microsystems, Eindhoven University of Technology, 3Applied Stem Cell Technologies, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente

JoVE 56334


 Bioengineering

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes

JoVE 55709


 Cancer Research

Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood

1Department of Clinical Research, University of Bern, 2Graduate School for Cellular and Biomedical Sciences, University of Bern, 3Immunology Research Centre, St. Vincent's Hospital Melbourne, 4Institute of Pathology, University of Bern, 5Institute of Molecular Animal Breeding and Biotechnology, Ludwig-Maximilian University

JoVE 56227


 Medicine

Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta

1Yale Cardiovascular Research Center, Section of Cardiovascular Medicine, Department of Internal Medicine, Yale University School of Medicine, 2Department of Neurology, Yale University School of Medicine, 3Department of Neurology, Xinhua Hospital, Shanghai Jiaotong University School of Medicine

JoVE 56039


 Developmental Biology

Fluorescent Dye Labeling of Erythrocytes and Leukocytes for Studying the Flow Dynamics in Mouse Retinal Circulation

1National Healthcare Group Eye Institute, Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), Singapore National Eye Center, 3School of Material Science and Engineering, Nanyang Technological University, 4Department of Ophthalmology, Yong Loo Lin School of Medicine, National University Health Systems, National University of Singapore, 5Ophthalmology Academic Clinical Research Program, DUKE-NUS Graduate Medical School

JoVE 55495


 Medicine

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

JoVE 55988


 Developmental Biology

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

1Institute of Immunobiology, Kantonsspital St. Gallen, 2Internal Medicine V, Medical University of Innsbruck, 3Tyrolean Cancer Research Institute (TKFI), 4Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology, University Clinic Bonn (UKB)

JoVE 55634


 Cancer Research

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

1Institute of Molecular Biology and Biochemistry, Medical University of Graz

JoVE 55486


 Biology

Modeling Ovarian Cancer Multicellular Spheroid Behavior in a Dynamic 3D Peritoneal Microdevice

1School of Biological Sciences, University of Hong Kong, 2Department of Mechanical Engineering, University of Hong Kong, 3Department of Medicine, University of Hong Kong, 4Department of Biomedical Sciences, Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong

JoVE 55337


 Bioengineering

Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function

1Department of Surgery, Baltimore Veterans Affairs Medical Center, 2Department of Surgery, University of Maryland School of Medicine, 3Department of Physiology, University of Maryland School of Medicine, 4Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine

JoVE 55201


 Biology

Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

1Department of Intensive Care Medicine, University Hospital Aachen, 2Institute of Biochemistry and Molecular Biology, University Hospital Aachen, 3Department of Radiology, German Cancer Research Center, 4Department of Thoracic and Cardiovascular Surgery, University Hospital Aachen, 5Department of Vascular Biology, Institute for Stroke and Dementia Research (ISD), Klinikum der Universität München, 6Deutsches Zentrum für Herz-/Kreislaufkrankheiten (DZHK), Munich Heart Alliance

JoVE 55192


 Medicine

Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion

1Laboratory for Physiology, Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center, 2Department of Internal Medicine, Institute for Cardiovascular Research (ICaR-VU), VU University Medical Center

JoVE 54912


 Medicine

Construction of a Multilayered Mesenchymal Stem Cell Sheet with a 3D Dynamic Culture System

1Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, 2Department of Developmental and Regenerative Biology, Jinan University, 3Nansha College Preparatory Academy, 4Department of Cardiology, First Affiliated Hospital of Jinan University

JoVE 58624


 Bioengineering

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