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Ethylene Glycol: A colorless, odorless, viscous dihydroxy alcohol. It has a sweet taste, but is poisonous if ingested. Ethylene glycol is the most important glycol commercially available and is manufactured on a large scale in the United States. It is used as an antifreeze and coolant, in hydraulic fluids, and in the manufacture of low-freezing dynamites and resins.

Hydrogel Synthesis

JoVE 10486

Source: Amber N. Barron, Ashlea Patterson, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT


Hydrogels are a versatile class of cross-linked polymers produced through relatively simple procedures and with generally inexpensive materials. They can be formed from solution and…

 Materials Engineering

Dean-Stark Trap

JoVE 10340

Source: Vy M. Dong and Jan Riedel, Department of Chemistry, University of California, Irvine, CA


A Dean-Stark trap is a special piece of glassware, which allows the collection of water during a reaction through an azeotropic distillation. The desire to collect water from a reaction can have various reasons. It can drive the equilibria in…

 Organic Chemistry II

Assembly of a Reflux System for Heated Chemical Reactions

JoVE 5516

Source: Laboratory of Dr. Philip Miller — Imperial College London


Many chemical experiments require elevated temperatures before any reaction is observed, however heating solutions of reactants can lead to loss of reactants and/or solvent via evaporation if their boiling points are sufficiently low. In order to ensure no loss of…

 Organic Chemistry

Efficiency of Liquid-liquid Extraction

JoVE 10426

Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA


Liquid-liquid extraction (LLE) is a separation technique used instead of distillation when either: (a) the relative volatilities of the compounds to be separated are very similar; (b) one or more of the mixture…

 Chemical Engineering

Conducting Reactions Below Room Temperature

JoVE 10224

Source: Laboratory of Dr. Dana Lashley - College of William and Mary


Demonstration by: Matt Smith


When new bonds are formed in the course of a chemical reaction, it requires that the involved species (atoms or molecules) come in very close proximity and collide into one another. The collisions between…

 Organic Chemistry

Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit

1Protein Crystallization Lab, Emerald Bio, 2Molecular Biology Lab, Emerald Bio, 3Scientific Sales Representative, Emerald Bio, 4Group Leader II, Emerald Bio, 5Group Leader I, Emerald Bio, 6Chair of Advisory Board, Emerald Bio, 7Director of Multi-Target Services, Emerald Bio, 8Senior Project Leader, Emerald Bio, 9Project Leader II & SSGCID Site Manager, Emerald Bio

JoVE 4225

 Immunology and Infection

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

1Department of Cellular and Molecular Physiology, Yale University School of Medicine, 2Nanobiology Institute, Yale University, 3Department of Molecular Biophysics and Biochemistry, Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales, Centre National de la Recherche Scientifique (CNRS)

JoVE 54349

 Neuroscience

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

1Biohybrid Materials, Department of Bioproducts and Biosystems, Aalto University, 2University of Jyväskylä, Nanoscience Center, Department of Biological and Environmental Science, University of Jyväskylä, 3University of Jyväskylä, Nanoscience Center, Department of Physics, University of Jyväskylä, 4HYBER Center of Excellence, Department of Applied Physics, Aalto University

JoVE 60313

 Chemistry

Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies

1Manchester Institute of Biotechnology & School of Chemistry, University of Manchester, 2School of Engineering, University of Liverpool, 3School of Electrical and Electronic Engineering, University of Manchester, 4School of Science and Technology, Nottingham Trent University

JoVE 56967

 Bioengineering

Polymer Microarrays for High Throughput Discovery of Biomaterials

1Laboratory of Biophysics and Surface Analysis, University of Nottingham, 2School of Molecular Medical Sciences, University of Nottingham, 3David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology

JoVE 3636

 Bioengineering

Comprehensive Autopsy Program for Individuals with Multiple Sclerosis

1Department of Neurosciences, Lerner Research Institute, Cleveland Clinic, 2Department of Biomedical Engineering, Lerner Research Institute, Cleveland Clinic, 3Mellen Center for Treatment and Research in Multiple Sclerosis, Neurological Institute, Cleveland Clinic

JoVE 59511

 Neuroscience
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