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Fura-2: A fluorescent calcium chelating agent which is used to study intracellular calcium in many tissues. The fluorescent and chelating properties of Fura-2 aid in the quantitation of endothelial cell injury, in monitoring Atp-dependent calcium uptake by membrane vesicles, and in the determination of the relationship between cytoplasmic free calcium and oxidase activation in rat neutrophils.

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

1Institute of Molecular Biology and Biochemistry, Medical University of Graz

JoVE 55486


 Biology

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

1Department of Microbiology and Immunology, University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, State University of New York

JoVE 51008


 Immunology and Infection

Isolation of Retinal Arterioles for Ex Vivo Cell Physiology Studies

1Centre for Experimental Medicine, Queen's University of Belfast, 2Centre for Biomedical Sciences (Education), Queen's University of Belfast, 3Department of Pharmaceutical Chemistry and Pharmacognosy, Naresuan University, 4School of Medicine, Dentistry and Biomedical Sciences, Queen's University of Belfast

Video Coming Soon

JoVE 57944


 JoVE In-Press

Calcium Imaging in Neurons

JoVE 5203

Calcium ions play an integral role in neuron function: They act as intracellular signals that can elicit responses such as altered gene expression and neurotransmitter release from synaptic vesicles. Within the cell, calcium concentration is highly dynamic due to the presence of pumps that selectively transport these ions in response to a variety of signals. Calcium imaging takes advantage of intracellular calcium flux to directly visualize calcium signaling in living neurons.This video begins with an overview of the key reagents used for this technique, known as calcium indicator dyes. The discussion includes an introduction to the commonly used dye Fura-2 and some basic principles behind how both ratiometric and non-ratiometric calcium indicators work. Next, a typical calcium imaging experiment is presented, from preparing the cells and dye to capturing and analyzing the fluorescent images. Finally, several experimental applications of calcium imaging are provided, such as the study of neuronal network activity and sensory processing.


 Neuroscience

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University

Video Coming Soon

JoVE 57629


 JoVE In-Press

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

1Department of Pharmacology and Experimental Therapeutics, University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Life Sciences Institute, University of Michigan, 3Department of Biomedical & Pharmaceutical Sciences, Chapman University, School of Pharmacy, Rinker Health Science campus

JoVE 52853


 Neuroscience

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

1Institute for Ophthalmic Research, University of Tübingen, 2Graduate School of Cellular & Molecular Neuroscience, University of Tübingen, 3Bernstein Centre for Computational Neuroscience, University of Tübingen, 4Molecular Genetics Laboratory, University of Tübingen, 5Centre for Ophthalmology, University of Tübingen

JoVE 52588


 Neuroscience

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