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Galectin 1: A galectin found abundantly in smooth muscle (Muscle, Smooth) and Skeletal muscle and many other tissues. It occurs as a homodimer with two 14-kDa subunits.

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

1Department of Chemical and Biomolecular Engineering, Ohio University, 2Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, 3Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School

JoVE 51023


 Bioengineering

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

1INSERM U970, Université Paris Descartes, 2Equipe Labellisée Ligue Contre le Cancer, 3Department of Immunology, Hopital Européen Georges Pompidou, 4Department of Medical Urology, Hopital Européen Georges Pompidou, 5Department of Pathology, Hopital Européen Georges Pompidou, 6Université Paris Diderot Paris 7, 7Department of medical oncology, Hopital Européen Georges Pompidou

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JoVE 56606


 JoVE In-Press

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

1Centro Cardiologico Monzino IRCCS, 2Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, 3Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, 4Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

JoVE 55762


 Biochemistry

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

1Institute of Immunobiology, Kantonsspital St. Gallen, 2Internal Medicine V, Medical University of Innsbruck, 3Tyrolean Cancer Research Institute (TKFI), 4Medical Clinic III, Oncology, Hematology, Immunoncology and Rheumatology, University Clinic Bonn (UKB)

JoVE 55634


 Cancer Research

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Surgical Training for the Implantation of Neocortical Microelectrode Arrays Using a Formaldehyde-fixed Human Cadaver Model

1Wyss Center for Bio and Neuroengineering, Geneva, 2Division of Neurology, Department of Clinical Neuroscience, Geneva University Hospitals, 3Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, 4Division of Neurosurgery, Department of Clinical Neuroscience, Geneva University Hospitals, 5Clinical Anatomy Research Group, Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva

JoVE 56584


 Medicine

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Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud

JoVE 56337


 Cancer Research

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University

JoVE 56597


 Bioengineering

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

JoVE 56474


 Biochemistry

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In Vivo Evaluation of Fracture Callus Development During Bone Healing in Mice Using an MRI-compatible Osteosynthesis Device for the Mouse Femur

1Institute of Orthopedic Research and Biomechanics, University Medical Center Ulm, 2RISystem, 3Core Facility Small Animal MRI, University Medical Center Ulm, 4Department of Traumatology, Hand-, Plastic-, and Reconstructive Surgery, University Medical Center Ulm

JoVE 56679


 Medicine

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Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA

JoVE 56203


 Biochemistry

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