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Hazardous Waste: Waste products which, upon release into the atmosphere, water or soil, cause health risks to humans or animals through skin contact, inhalation or ingestion. Hazardous waste sites which contain hazardous waste substances go here.

Proper Waste Disposal

JoVE 10403

Robert M. Rioux and Taslima A. Zaman, Pennsylvania State University, Pennsylvania, PA

Users are responsible for the proper disposal of the waste generated during their work. Improper waste disposal may severely endanger public health and/or the environment. The handling of hazardous waste must be regulated from the moment of generation until its disposal at its offsite final destination facility. A waste management system must be devised before work begins on any laboratory activity. Users must comply with the rules and regulations of their institute's Environmental Health and Safety (EHS) office, which develops and implements proper waste management systems satisfying diverse regulations and standards, such as those imposed by the Occupational Safety and Health Administration (OSHA).


 Lab Safety

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

1Department of Biology, University of the South, 2School of Biological Sciences, Louisiana Tech University, 3School of Medicine, Louisiana State University Health Sciences Center, 4Department of Biology, Furman University, 5Department of Computer Science, Louisiana Tech University, 6Clemson University

JoVE 55754


 Biology

Using a Microfluidics Device for Mechanical Stimulation and High Resolution Imaging of C. elegans

1Department of Molecular and Cellular Physiology, Stanford University, 2Department of Mechanical Engineering, Stanford University, 3Department of Bioengineering, Stanford University, 4Group of Neurophotonics and Mechanical Systems Biology, The Institute of Photonic Sciences (ICFO)

JoVE 56530


 Neuroscience

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

1Department of Health, Animal Science and Food Safety, University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE, Université de Tours, INRA, 4PAO, INRA, 5Clinique des Animaux de Compagnie et des Équidés, Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

JoVE 55242


 Developmental Biology

Working with Hot and Cold Sources

JoVE 10366

Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA

Working with extreme temperatures, both high and low, is an integral part of many laboratory operations. For many, mentioning a laboratory instantly evokes the mental picture of a Bunsen burner. Bunsen burners and hot plates are used extensively in small and large operations in research laboratories and industries, thus making it necessary for all users to be aware of their safe handling procedures. Hot plates and Bunsen burners are high temperature heat sources, while low temperatures are obtained using dry ice and cryogenic liquids, such as liquid nitrogen. Both dry ice and liquid nitrogen can pose significant hazards to the user if not handled carefully.


 Lab Safety

Proper Personal Protective Equipment

JoVE 10402

Robert M. Rioux & William A Elliott, Pennsylvania State University, University Park, PA

Hazards are many and varied in the laboratory, but the right choice of PPE can make the laboratory a safe place to work.


 Lab Safety

Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

1Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet, 2Division of Medicine, Department of Public Health and Clinical Medicine, Umeå University, 3Divison of Infectious Diseases, Department of Clinical Microbiology, Umeå University

JoVE 55222


 Immunology and Infection

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

1Centre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, 2Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, 3Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, 4Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services, Australian National University Medical School, 5Infection Management Services, Australian National University Medical School, 6Department of Microbiology and Infectious Diseases, St. Vincent's Hospital, 7Department of Infectious Diseases, Peter MacCallum Cancer Centre

JoVE 56714


 Medicine

Handling Chemical Spills

JoVE 10371

Source: Robert M. Rioux & Taslima Zaman, Pennsylvania State University, University Park, PA

Since chemicals are commonly used for laboratory research purposes, it is extremely important to be adequately prepared to handle chemical spills or accidental release of hazardous chemicals, which can happen at any time. No matter how minor a spill may be, the inability to respond in an emergency situation could severely endanger public health or the environment. All chemical spills must be properly disposed of, satisfying diverse regulations and standards, such as those of Resource Conservation and Recovery Act (RCRA) and the Emergency Planning and Community Right to Act of 1986, which are administered by the US Environmental Protection Agency (EPA).


 Lab Safety

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine

JoVE 55561


 Biochemistry

X-ray Fluorescence (XRF)

JoVE 5498

Source: Laboratory of Dr. Lydia Finney — Argonne National Laboratory

X-ray fluorescence is an induced, emitted radiation that can be used to generate spectroscopic information. X-ray fluorescence microscopy is a non-destructive imaging technique that uses the induced fluorescence emission of metals to identify and quantify their spatial distribution.


 Analytical Chemistry

Functionalization and Dispersion of Carbon Nanomaterials Using an Environmentally Friendly Ultrasonicated Ozonolysis Process

1Aerospace Division, Defence Science and Technology Group, 2Maritime Division, Defence Science and Technology Group, 3Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, 4Department of Mechanical Engineering, University of Delaware, 5Department of Materials Science and Engineering, University of Delaware, 6Center for Composite Materials, University of Delaware

JoVE 55614


 Environment

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