JoVE Biology
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
1Green Center for Systems Biology, University of Texas Southwestern Medical Center
JoVE Biology
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
1Architecture et Fonction des Macromolécules Biologiques (AFMB), Aix-Marseille Université, 2iBiTec-S, Service d'Ingénierie Moléculaire des Protéines (SIMOPRO), Commissariat à l'énergie atomique et aux énergies alternatives (CEA) Saclay, France
JoVE Biology
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
1Chair of Chemistry of Biogenic Resources, Technische Universität München
JoVE Immunology and Infection
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research, University of Toronto, 3Antibiome Center, University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology, The University of Chicago
JoVE In-Press
3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening
1Department of Biomedical Engineering, School of Medicine, Tsinghua University, 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, 3School of Life Sciences, Tsinghua University
JoVE Immunology and Infection
High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity
1Department of Pathology, Beth Israel Deaconess Medical Center
JoVE Biology
High-Throughput, Multi-Image Cryohistology of Mineralized Tissues
1Department of Reconstructive Sciences, University of Connecticut Health Center, 2Department of Computer Science and Engineering, University of Connecticut, 3Department of Orthopaedic Surgery, University of Connecticut Health Center, 4Department of Orthopaedics, University of Rochester
JoVE Biology
High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
1Department of Pharmacology, Vanderbilt University School of Medicine, 2Department of Anesthesiology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine
JoVE Immunology and Infection
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
1Inserm U1019 - CNRS UMR 8024, Institut Pasteur de Lille, Université de Lille
JoVE Biology
RNA Secondary Structure Prediction Using High-throughput SHAPE
JoVE Environment
High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
1BioEnergy Science Center, National Renewable Energy Laboratory
JoVE In-Press
High-throughput Analysis of Locomotor Behavior in the Drosophila Island Assay
1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, 2Centre for Molecular and Biomolecular Informatics, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 3Department of Neurology, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center
JoVE Neuroscience
High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
1Monell Chemical Senses Center
JoVE Biochemistry
High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
1Department for Plant and Environmental Sciences, University of Copenhagen, 2School of Agriculture, Food and Rural Development, Newcastle University
JoVE Medicine
High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
1Institute for Neuroscience and Muscle Research, The Kids Research Institute at the Children's Hospital at Westmead, The University of Sydney, 2Flow Cytometry Centre, Westmead Millennium Institute for Medical Research
JoVE Biology
A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
1Department of Biological Sciences, University of Notre Dame
JoVE In-Press
Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
1Arabidopsis Biological Resource Center, Center for Applied Plant Sciences, The Ohio State University, 2Department of Molecular Genetics, Center for Applied Plant Sciences, The Ohio State University
JoVE Chemistry
A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
1Department of Chemistry, The Scripps Research Institute, 2Worm Institute of Research and Medicine (WIRM), The Scripps Research Institute
JoVE Biology
Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
1Center for Human Genetic Research and Department of Medicine, Massachusetts General Hospital and Harvard Medical School, 2Department of Earth, Atmospheric, and Planetary Sciences, Massachusetts Institute of Technology
JoVE Immunology and Infection
Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery
1Institute of Biology, Leiden University, 2ZF-screens BV, 3Life Science Methods BV
JoVE Biology
High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
1Raymond and Beverly Sackler Foundation, New Jersey, 2Histopathology and Imaging Shared Resource, Rutgers University, 3Rutgers Cancer Institute of New Jersey, Rutgers University, 4School of Natural Sciences, Institute for Advanced Study, New Jersey
JoVE Genetics
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
1Cancer & Stem Cell Biology Programme, Duke-NUS Medical School
JoVE Bioengineering
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
1Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston
JoVE Immunology and Infection
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur
JoVE Biology
DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
1Department of Cell Biology, Yale University School of Medicine
JoVE Immunology and Infection
High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 2Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A&M University System Health Science Center, 3University of California, Irvine, 4Department of Medical Microbiology & Immunology, School of Medicine, University of California, Davis
JoVE Biology
Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
1School of Life Sciences, Arizona State University, 2Biodesign Institute, Arizona State University
JoVE Bioengineering
High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
1Application Development, Akonni Biosystems, Inc., 2Manufacturing, Akonni Biosystems, Inc., 3Engineering, Akonni Biosystems, Inc., 4Research & Development, Akonni Biosystems, Inc.
JoVE Bioengineering
CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells
1Department of Biological Engineering, Massachusetts Institute of Technology, 2Environmental Toxicology, Chulabhorn Graduate Institute, 3Department of Biomedical Engineering, University of Minnesota
JoVE Biology
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
1Department of Neurology, Massachusetts General Hospital
JoVE Biology
Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
1Institute for Hepatitis and Virus Research, 2Department of Microbiology and Immunology, Thomas Jefferson University, 3Drexel University College of Medicine, 4Van Andel Research Institute, 5Institute for Hepatitis and Virus Research, Serome Biosciences Inc.
JoVE In-Press
Precise, High-throughput Analysis of Bacterial Growth
1Graduate School of Life and Environmental Sciences, University of Tsukuba
JoVE Immunology and Infection
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
1Department of Microbiology, Infectiology and Immunology, Université de Montréal, 2Department of Pharmacology and Physiology, Université de Montréal, 3Molecular Biology Program, Université de Montréal
JoVE Bioengineering
A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces
1Department of Bioengineering, University of Illinois at Urbana–Champaign
JoVE Developmental Biology
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
1The Department of Human Nutrition, Foods, and Exercise, Virginia Tech, 2The Metabolic Phenotyping Core, Virginia Tech, 3Seahorse Bioscience
JoVE Biology
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, South Korea
JoVE Chemistry
Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
1Department of Chemistry, University at Albany-SUNY
JoVE Immunology and Infection
A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis
1Department of Medicine, University of Alabama at Birmingham
JoVE Biology
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
1Laboratory of Translational Genomics, Centre for Integrative Biology, University of Trento, 2High Throughput Screening Core Facility, Centre for Integrative Biology, University of Trento
JoVE Biology
Quantitative and Automated High-throughput Genome-wide RNAi Screens in C. elegans
1Centre d’Immunologie de Marseille-Luminy, Université de la Méditerranée
JoVE Biology
High-throughput Titration of Luciferase-expressing Recombinant Viruses
1Center for Innovative Cancer Research, Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, 3Faculty of Medicine, University of Ottawa
JoVE Environment
High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
1Natural Resource Ecology Laboratory, Colorado State University, 2Biosciences Division, Oak Ridge National Laboratory, 3Department of Bioengineering, University of Colorado
JoVE Bioengineering
High-throughput Identification of Bacteria Repellent Polymers for Medical Devices
1School of Chemistry, EaStCHEM, University of Edinburgh, 2School of Biological Sciences, University of Edinburgh, 3Critical Care, NHS Lothian, Royal Infirmary of Edinburgh
JoVE Environment
Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy
1Lawrence Berkeley National Laboratory, The Molecular Foundry
JoVE Biology
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
1Thayer School of Engineering, Dartmouth College, 2Institute for Immunology and Informatics, University of Rhode Island, 3Department of Computer Science, Dartmouth College
JoVE Bioengineering
High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
1Department of Chemical and Biological Engineering, Iowa State University, 2Department of Chemistry, Iowa State University
JoVE Immunology and Infection
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
1Division of Molecular Microbiology, College of Life Sciences, University of Dundee
JoVE Genetics
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
1Department of Plant Sciences, University of Tennessee, Knoxville, 2Center for Renewable Carbon, University of Tennessee, Knoxville, 3Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville
JoVE Neuroscience
FIM Imaging and FIMtrack: Two New Tools Allowing High-throughput and Cost Effective Locomotion Analysis
1Institute of Neuro and Behavioral Biology, Westfälische Wilhelms-Universität Münster, 2Department of Mathematics and Computer Science, Westfälische Wilhelms-Universität Münster
JoVE Neuroscience
Two Algorithms for High-throughput and Multi-parametric Quantification of Drosophila Neuromuscular Junction Morphology
1Department of Human Genetics, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, 2Microscopical Imaging Centre (MIC), Radboud University Medical Center, 3Department of Biology, Universidad Autónoma de Madrid, 4Department of Clinical and Experimental Medicine, Linköping University, 5Department of Pathology, Radboud University Medical Center
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