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 JoVE Immunology and Infection

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

1Focal Area Infection Biology, Biozentrum, University of Basel, 2Centre d’Immunologie de Marseille-Luminy, Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical, Universidad de Navarra, 4BioDataAnalysis GmbH


JoVE 54263

 JoVE In-Press

Indacenodithienothiophene-Based Ternary Organic Solar Cells: Concept, Devices and Optoelectronic Analysis

1Institute of Materials for Electronics and Energy Technology (I-MEET), Friedrich-Alexander-University Erlangen-Nuremberg, 2Macromolecular Chemistry Group (buwmakro) and Institute for Polymer Technology, Bergische Universität Wuppertal, 3Department of Materials Science Engineering, University of Ioannina, 4Advent Technologies SA, 5National Hellenic Research Foundation (NHRF), 6Bavarian Center for Applied Energy Research (ZAE Bayern)

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JoVE 54007

 JoVE Chemistry

Low-energy Cathodoluminescence for (Oxy)Nitride Phosphors

1Graduate School of Pure and Applied Science, University of Tsukuba, 2CNRS — Saint-Gobain, UMI 3629, Laboratory for Innovative Key Materials and Structures (LINK), 3Nano Device Characterization Group, National Institute for Materials Science (NIMS), 4Sialon Unit, National Institute for Materials Science (NIMS)


JoVE 54249

 JoVE Bioengineering

A Step Beyond BRET: Fluorescence by Unbound Excitation from Luminescence (FUEL)

1Plate-Forme d'Imagerie Dynamique, Imagopole, Institut Pasteur, 2Department of Radiation Oncology, Stanford School of Medicine, 3Service Hospitalier Frédéric Joliot, Institut d'Imagerie Biomédicale, 4Vanderbilt School of Medicine, 5The Walter & Eliza Hall Institute of Medical Research, 6Unité INSERM U786, Institut Pasteur, 7Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur


JoVE 51549

 JoVE Biology

A Guide to Modern Quantitative Fluorescent Western Blotting with Troubleshooting Strategies

1Division of Neurobiology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 2Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, 3Centre for Integrative Physiology, University of Edinburgh, 4Euan MacDonald Centre for Motor Neurone Disease Research, University of Edinburgh


JoVE 52099

 JoVE Medicine

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

1Bionics Institute, 2Department of Anatomical Pathology, St Vincent's Hospital Melbourne, 3Department of Pathology, University of Melbourne, 4Medical Bionics Department, University of Melbourne


JoVE 50411

 JoVE Bioengineering

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University


JoVE 3884

 Science Education: Essentials of Genetics

Genome Editing

JoVE Science Education

A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be introduced into the targeted DNA region. In this video, JoVE explains the principles behind genome editing, emphasizing how this technique relates to DNA repair mechanisms. Then, three major genome editing methods—zinc finger nucleases, TALENs, and the CRISPR-Cas9 system—are reviewed, followed by a protocol for using CRISPR to create targeted genetic changes in mammalian cells. Finally, we discuss some current research that applies genome editing to alter the genetic material in model organisms or cultured cells.

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 JoVE Immunology and Infection

Electroporation of Functional Bacterial Effectors into Mammalian Cells

1Biological Sciences Division, Pacific Northwest National Laboratory, 2Environmental Molecular Science Laboratory, Pacific Northwest National Laboratory, 3Structural Proteomics Group, Ontario Center for Structural Proteomics, University of Toronto, 4Center for Bioproducts and Bioenergy, Washington State University


JoVE 52296

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 JoVE Immunology and Infection

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

1Department of Biology, San Diego State University, 2Computational Science Research Center, San Diego State University, 3Bioinformatics and Medical Informatics Research Center, San Diego State University, 4Department of Mathematics and Statistics, San Diego State University, 5Department of Computer Science, San Diego State University, 6Mathematics and Computer Science Division, Argonne National Laboratory, 7SPARC Committee, Broad Institute


JoVE 52854

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