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Infertility, Female: Diminished or absent ability of a female to achieve conception.

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

1Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología-Universidad Nacional Autónoma de México, 2Math and Sciences Department, Edison State College

JoVE 50344


 Biology

A Novel Use of Three-dimensional High-frequency Ultrasonography for Early Pregnancy Characterization in the Mouse

1Devision of Repreoductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Baylor College of Medicine, 2Mouse Phenotyping Core, Baylor College of Medicine, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Department of Molecular and Cellular Biology, Baylor College of Medicine

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JoVE 56207


 JoVE In-Press

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

JoVE 4372


 Medicine

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Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

1Department of Health, Animal Science and Food Safety, University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE, Université de Tours, INRA, 4PAO, INRA, 5Clinique des Animaux de Compagnie et des Équidés, Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

JoVE 55242


 Developmental Biology

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Recording Mouse Ultrasonic Vocalizations to Evaluate Social Communication

1Human Genetics and Cognitive Functions, University Paris Diderot, CNRS UMR 3571, Institut Pasteur, 2Neurophysiology and Behavior, University Pierre et Marie Curie Paris 6, CNRS UMR 7102, 3Bio Image Analysis, CNRS URA 2582, Institut Pasteur

JoVE 53871


 Behavior

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Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity

1Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Greenville Hospital System, 2Department of Pathology, Duke University Health System, 3Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Duke University

JoVE 4313


 Medicine

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Drosophila Courtship Conditioning As a Measure of Learning and Memory

1Department of Human Genetics, Radboud University Medical Center, 2Radboud Institute of Molecular Life Sciences, Radboud University, 3Donders Institute for Brain, Cognition, and Behaviour, Centre for Neuroscience, Radboud University, 4Research Institute of Molecular Pathology, Vienna, Austria, 5Department for Health Evidence, Radboud University Medical Center, 6Janelia Research Campus, Howard Hughes Medical Institute, 7Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Western University, 8Department of Biology, Faculty of Science, Western University

JoVE 55808


 Neuroscience

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Fundamentals of Breeding and Weaning

JoVE 10293

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

Millions of mice and rats are bred for use in biomedical research each year. Worldwide, there are several large commercial breeding facilities that supply mice to research laboratories, but many facilities choose to also breed mice and rats in-house to reduce costs and increase research options. When breeding in the animal facility, researchers are able to manipulate the genetics of the animals, time the pregnancies to meet the needs of the research, and work with embryos and neonates as required. Mice and rats can be bred in a variety of schemes and methods. Technical procedures, such as the use of vaginal cytology, visualization of the vaginal area, and observation of copulatory plugs, have been developed to assist with the synchronization of breeding to correspond to research requirements. This manuscript is an overview of the basic fundamentals of mouse and rat breeding and technical procedures used. More detailed descriptions of the complex breeding schemes, and the full description of the methods for vaginal cytology, are available in the list of references.


 Lab Animal Research

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Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

1Department of Bioengineering & Therapeutic Sciences, University of California, San Francisco, 2Department of Obstetrics, Gynecology & Reproductive Sciences, University of California, San Francisco, 3Keck School of Medicine, University of Southern California

JoVE 51745


 Bioengineering

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Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

1Comprehensive Transplant Center, Feinberg School of Medicine, Northwestern University, 2Department of Surgery, Feinberg School of Medicine, Northwestern University, 3Department of Biomedical Engineering, Northwestern University, 4Simpson Querrey Institute for BioNanotechnology in Medicine, Northwestern University, 5Department of Internal Medicine, University of New Mexico HSC, 6Department of Pathology, University of New Mexico HSC, 7Department of Chemical and Biological Engineering, Northwestern University, 8Chemistry of Life Processes Institute, Northwestern University, 9Department of Surgery, Jesse Brown VA Medical Center

JoVE 53271


 Bioengineering

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Mass Production of Genetically Modified Aedes aegypti for Field Releases in Brazil

1Oxitec Ltd, 2Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, 3Departamento de Epidemiologia, Universidade de São Paulo, 4Moscamed Brasil, 5Deptartment of Zoology, University of Oxford, 6Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular (INCT-EM)

JoVE 3579


 Environment

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Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus

1Department of Forest Protection, Zhejiang Agricultural & Forestry University, 2Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, 3College of Plant Protection, Shandong Agricultural University, 4Institute of Forest Protection, Chinese Academy of Forestry, 5Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University

JoVE 54842


 Biology

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Moderate Prenatal Alcohol Exposure and Quantification of Social Behavior in Adult Rats

1Department of Psychology, University of New Mexico, 2Department of Neurosciences, University of New Mexico, 3Department of Psychiatry and Behavioral Sciences, University of New Mexico, 4Canadian Centre for Behavioural Neuroscience, University of Lethbridge

JoVE 52407


 Behavior

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Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

1Department of Chemical Engineering and Centre for Regenerative Medicine, University of Bath, 2MRC Centre for Drug Safety Science and Institute of Translational Medicine, University of Liverpool, 3Mechanical Engineering, University College London, 4Department of Applied Mathematics, Liverpool John Moores University

JoVE 53431


 Bioengineering

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