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Lac Repressors: Bacterial repressor proteins that bind to the Lac operon and thereby prevent the synthesis of proteins involved in catabolism of Lactose. When lactose levels are high lac repressors undergo an allosteric change that causes their release from the DNA and the resumption of lac operon transcription.
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Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota

JoVE 50762


 Biology

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Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples

1Center for Molecular Medicine Cologne (CMMC), University of Cologne, 2Cologne Center for Genomics (CCG), University of Cologne, 3Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), Research Center and Systems Biology of Ageing Cologne, University of Cologne

JoVE 56186


 Genetics

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Inactivation of mTor: A Tool to Investigate Meiotic Progression and Translational Control During Bovine Oocyte Maturation

1BVN Neustadt/Aisch, 2Faculty of Veterinary Medicine, Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus-Liebig-University Giessen, 3Reproductive Cell Biology Unit, Leibniz Institute for Farm Animal Biology, 4Reproductive Biochemistry Unit, Leibniz Institute for Farm Animal Biology

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JoVE 53689


 JoVE In-Press

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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

JoVE 55520


 Developmental Biology

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