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Leukemia, Experimental: Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as Viruses; Radiation; or by Transplantation of leukemic tissues.

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

1Department of Pediatric Oncology/Hematology, VU University Medical Center, 2Department of Hematology, VU University Medical Center, 3Department of Medical Oncology, VU University Medical Center, 4Department of Clinical Genetics, VU University Medical Center, 5Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, 6Amsterdam Immunology and Rheumatology Center, VU University Medical Center, 7Princess Máxima Center for Pediatric Oncology, 8Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, 9Institute of Nanoscience and Nanotechnology, CNR-Nano

JoVE 54714


 Cancer Research

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center, 3Department of Cell and Molecular Biology, St. Jude Children's Research Hospital

Video Coming Soon

JoVE 56418


 JoVE In-Press

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

1Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, 3Department of Pathology, UT Health Science Center at San Antonio, 4Department of Microbiology, UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center, UT Health Science Center at San Antonio

JoVE 50752


 Immunology and Infection

Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response

1Department of Oncological Sciences and Huntsman Cancer Institute, University of Utah School of Medicine, 2Department of Dermatology, University of Utah Health Sciences Center, Salt Lake City

JoVE 55712


 Cancer Research

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology, Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy, Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology, Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine, University of Oslo

JoVE 54615


 Immunology and Infection

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

1Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Center for Drug Evaluation and Research, US Food and Drug Administration

JoVE 52650


 Immunology and Infection

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

1Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC

JoVE 56122


 Immunology and Infection

An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection

1Genetic Resources Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 2MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, 3State Key Laboratory of molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences

Video Coming Soon

JoVE 56515


 JoVE In-Press

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

1Division of Hematology, Davidoff Cancer Center, Rabin Medical Center, 2The Center of Nanoscience and Nanotechnology, Tel Aviv University, 3Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, 4Department of Leukemia, The University of Texas MD Anderson Cancer Center

JoVE 53300


 Bioengineering

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

1Institute of Pathology, University Medical Center, Göttingen, 2Department of Hematology/Oncology, Goethe University of Frankfurt, 3Bioanalytical Mass Spectrometry Group, Max Planck Institute for Biophysical Chemistry, 4Bioanalytics, Institute of Clinical Chemistry, University Medical Center, Göttingen, 5German Cancer Consortium, 6German Cancer Research Center

JoVE 52435


 Medicine

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

1Department of Medicine, Weill Cornell Medical College, 2Institute for Computational Biomedicine, Weill Cornell Medical College, 3Department of Physiology and Biophysics, Weill Cornell Medical College, 4Department of Pathology, University of Michigan

JoVE 52246


 Biology

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

1Department of Medicine, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University, 2Department of Molecular Cardiology, Cleveland Clinic Foundation, 3Department of Cancer Biology, Cardeza Vascular Research Center, Sidney Kimmel Medical College, Thomas Jefferson University

JoVE 55621


 Developmental Biology

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

1Transfusion Research Center, Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences, Ghent University, 3Blood Service, Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care, KULeuven - University of Leuven

JoVE 55351


 Bioengineering

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, 2The Saban Research Institute of Children's Hospital, 3Department of Biochemistry and Molecular Medicine, University of Southern California

Video Coming Soon

JoVE 56333


 JoVE In-Press

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