JoVE Biochemistry
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
1Fischell Department of Bioengineering, University of Maryland, 2Department of Chemical and Biomolecular Engineering, University of Maryland
Libraries:
Collections of systematically acquired and organized information resources, and usually providing assistance to users. (Eric Thesaurus, http://www.eric.ed.gov/ accessed 2/1/2008)
JoVE Biology
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
JoVE Chemistry
Development of a Backbone Cyclic Peptide Library as Potential Antiparasitic Therapeutics Using Microwave Irradiation
1Department of Chemical and Systems Biology, Stanford University School of Medicine
JoVE Biology
Primer-Free Aptamer Selection Using A Random DNA Library
1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University
JoVE Chemistry
Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library
1Scripps Florida, The Scripps Research Institute
JoVE Biology
Large Insert Environmental Genomic Library Production
1Department of Microbiology and Immunology, University of British Columbia - UBC
JoVE Medicine
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
1Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences
JoVE Biology
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
JoVE Biology
Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
1Department of Biocatalysis, Institute of Catalysis, CSIC
JoVE Bioengineering
Haptic/Graphic Rehabilitation: Integrating a Robot into a Virtual Environment Library and Applying it to Stroke Therapy
1Department of Bioengineering, University of Illinois at Chicago and Rehabilitation Institute of Chicago, 2Sensory Motor Performance Program, Rehabilitation Institute of Chicago
JoVE Biology
Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
1Orthopaedics Research, University of Virginia, 2Biological Sciences, University of Delaware, 3Orthopaedic Surgery, University of Virginia
JoVE In-Press
3' End Sequencing Library Preparation with A-seq2
1Computational and Systems Biology, Biozentrum, University of Basel, 2Swiss Institute of Bioinformatics, Biozentrum, University of Basel
JoVE Biology
A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
1Green Center for Systems Biology, University of Texas Southwestern Medical Center
JoVE Biology
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
1Department of Medicine, Weill Cornell Medical College, 2Institute for Computational Biomedicine, Weill Cornell Medical College, 3Department of Physiology and Biophysics, Weill Cornell Medical College, 4Department of Pathology, University of Michigan
JoVE In-Press
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
1Sensors and Electron Devices Directorate, US Army Research Laboratory
JoVE Cancer Research
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
1Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, 2Division of Hematology/Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center
JoVE In-Press
Mapping Genome-Wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
1The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University
JoVE Biology
RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
1Children's Mercy Hospital and Clinics, School of Medicine, University of Missouri-Kansas City
JoVE Immunology and Infection
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
1The Recombinant Antibody Network, 2The Banting and Best Department of Medical Research, University of Toronto, 3Antibiome Center, University of California, San Francisco at Mission Bay, 4Department of Biochemistry and Molecular Biology, The University of Chicago
JoVE Biology
Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes
1Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 2Neuroscience and Behavior Program, University of Massachusetts, Amherst, 3Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst
JoVE Developmental Biology
Functional Cloning Using a Xenopus Oocyte Expression System
1Department of Biology, Shepherd University, 2Department of Biology, University of Virginia
JoVE Genetics
Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma
1Innovation Laboratory, Center for Molecular Oncology, Memorial Sloan Kettering Cancer Center, 2Flow-Sorting Core Facility, Weill Cornell Medical College, 3Department of Laboratory Medicine, University of Washington, 4Department of Physiology and Biophysics, Institute for Computational Biomedicine, Weill Cornell Medical College, 5Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, 6Department of Laboratory Medicine, Memorial Sloan Kettering Cancer Center
JoVE Biology
Automating ChIP-seq Experiments to Generate Epigenetic Profiles on 10,000 HeLa Cells
1Diagenode S.A., 2Diagenode Inc.
JoVE Biology
High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
1Department of Neurology, Massachusetts General Hospital
JoVE Biology
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
1Department of Internal Medicine, Division of Medical Oncology, Comprehensive Cancer Center, The Ohio State University, 2Department of Pharmacology, The Ohio State University
JoVE Medicine
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
1Asuragen, Inc.
JoVE In-Press
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
1Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science, University of Minnesota, 3Department of Plant Pathology, University of Minnesota
JoVE Immunology and Infection
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
1Department of Molecular Biology - Functional Genomics, Laimburg Research Centre
JoVE Biology
Chromatin Interaction Analysis with Paired-End Tag Sequencing (ChIA-PET) for Mapping Chromatin Interactions and Understanding Transcription Regulation
1Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, 2A*STAR-Duke-NUS Neuroscience Research Partnership, Singapore, 3Department of Biochemistry, National University of Singapore, Singapore
JoVE In-Press
Non-invasive Chromosome Screening (NICS) of Human Preimplantation Embryos: Sample Collection and Chromosomal Ploidy Analysis by MALBAC-NGS
1Reproductive Medicine Centre, Wuxi Maternity and Child Health Hospital Affiliated to Nanjing Medical University, 2Department of Clinical Research, Yikon Genomics Co. Ltd.
JoVE Genetics
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
1Stem Cell and Regenerative Biology, Genome Institute of Singapore, A*STAR, 2Computational and Systems Biology, Genome Institute of Singapore, A*STAR
JoVE Biology
Massively Parallel Reporter Assays in Cultured Mammalian Cells
1Broad Institute
JoVE Biology
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
1711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, 2The Henry M. Jackson Foundation, 3UES, Inc.
JoVE Biology
A Protocol for Computer-Based Protein Structure and Function Prediction
1Center for Computational Medicine and Bioinformatics, University of Michigan, 2Center for Bioinformatics and Department of Molecular Bioscience, University of Kansas
JoVE Biology
Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
1Regenerative Biology, Morgridge Institute for Research, 2Department of Cell & Regenerative Biology, University of Wisconsin, 3Department of Molecular, Cellular, & Regenerative Biology, University of California
JoVE Immunology and Infection
Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling
1Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology, Heidelberg University, 2Department of Infectious Diseases, Virology, Heidelberg University
JoVE In-Press
Chromatin Immunoprecipitation (ChIP) Protocol for Low-abundance Embryonic Samples
1Center for Molecular Medicine Cologne (CMMC), University of Cologne, 2Cologne Center for Genomics (CCG), University of Cologne, 3Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD) Research Center and Systems Biology of Ageing Cologne, University of Cologne
JoVE Biology
The ChIP-exo Method: Identifying Protein-DNA Interactions with Near Base Pair Precision
1Department of Molecular Physiology and Biophysics, Vanderbilt University
JoVE In-Press
The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell
1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children, Harvard Medical School, 2College of Life Sciences, Jilin University, 3Faculty of Health Sciences, University of Macau
JoVE Medicine
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
1Broad Institute of MIT and Harvard, 2Harvard University
JoVE Developmental Biology
Forward Genetic Approach to Uncover Stress Resistance Genes in Mice — A High-throughput Screen in ES Cells
1Department of Cell and Developmental Biology, University of Colorado Denver
JoVE Bioengineering
Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping
1Nanobioscience Constellation, SUNY Polytechnic Institute, Colleges of Nanoscale Science and Engineering
JoVE Medicine
VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
1Department of Medicine, Weill Cornell Medical College, 2Institute for Computational Biomedicine, Weill Cornell Medical College, 3Department of Pathology and Laboratory Medicine, Weill Cornell Medical College
JoVE Biology
Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center, The University of Texas Graduate School of Biomedical Sciences at Houston
JoVE Biology
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
1Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 2Chromatin Structure and Mobile DNA, The Francis Crick Institute
JoVE Biology
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
1Department of Biology and Pathology of Tumors, Unit of Molecular Biology, Platform of Immunomonitoring and Genetics, Centre Georges-François Leclerc
JoVE Biology
Purifying the Impure: Sequencing Metagenomes and Metatranscriptomes from Complex Animal-associated Samples
1Department of Biology, San Diego State University, 2DOE Joint Genome Institute, 3Department of Molecular, Cellular and Developmental Biology, University of Colorado, 4Department of Pediatrics, School of Medicine, University of Colorado
JoVE Biology
A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology - Campus North, 2Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus North, 3Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus South
JoVE Bioengineering
Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis
1School for Engineering of Matter, Transport and Energy, Arizona State University, 2Department of Neurology, Georgetown University Medical Center, 3Department of Pathology, Georgetown University Medical Center
JoVE In-Press
A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and in Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations
1Department of Neuroscience, Pharmacology and Child Health, University of Florence, 2INSERM, INMED, 3Aix-Marseille University, 4Plateforme Biologie Moléculaire et Cellulaire, INMED, 5Department of Neurology, Royal Children's Hospital, 6Murdoch Children's Research Institute, 7Department of Paediatrics, University of Melbourne, 8Plateforme postgenomique INMED, 9Department Molecular Medicine, University of Pavia, 10NIHR Biomedical Research Centre, Wellcome Trust Centre for Human Genetics, 11Department of Clinical Genetics, Oxford Radcliffe NHS Trust, 12IRCCS Casimiro Mondino Foundation, 13Research Institute of Molecular Pathology, 14IRCCS Stella Maris, 15Department of Pathology and Cell Biology, Columbia University
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