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Macrophage Activation: The process of altering the morphology and functional activity of macrophages so that they become avidly phagocytic. It is initiated by lymphokines, such as the macrophage activation factor (Maf) and the macrophage migration-inhibitory factor (Mmif), immune complexes, C3b, and various peptides, polysaccharides, and immunologic adjuvants.

Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry

1Renal, Vascular and Diabetes Research Lab, IIS-Fundaciòn Jiménez Dìaz, Autonoma University, 2Department of Physiology, Faculty of Medicine, Complutense University, 3Department of Immunology, Centro Nacional de Microbiologìa, Instituto de Salud Carlos III (ISCIII)

JoVE 54599


 Immunology and Infection

Polyethyleneimine-coated Iron Oxide Nanoparticles As a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

1Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Institute of Life Sciences, Southeast University, 2State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials & Devices, School of Biological Science & Medical Engineering, Southeast University, 3Key Laboratory of Ministry of Education for Developmental Genes & Human Diseases, Medical School, Southeast University

Video Coming Soon

JoVE 58660


 JoVE In-Press

Reliable and High Efficiency Extraction of Kidney Immune Cells

1Division of Nephrology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 2Division of Biomedical Sciences, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital, 3Division of Endocrinology, Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran's Hospital

JoVE 54368


 Immunology and Infection

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

1Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, 2Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health

JoVE 54918


 Immunology and Infection

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Phase Imaging and Machine Learning

1Department of Physics, University of Cambridge, 2Department of Physics, Korea Advanced Institute of Science and Technology, 3KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, 4Tomocube, Inc., 5Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 6Department of Biological Sciences, Korea Advanced Institute of Science and Technology

Video Coming Soon

JoVE 58305


 JoVE In-Press

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

1Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, 2Department of Medical Biotechnology, Yeungnam University, 3Department of Chemistry, Pukyong National University, 4Marine-integrated Bionics Research Center, Pukyong National University, 5Department of Biochemistry, Chungbuk National University, 6Department of Biomedical Sciences, University of Ulsan College of Medicine, Asan Medical Center

JoVE 57640


 Immunology and Infection

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

1University of California, Los Angeles, 2Centre for Biomedical Research, Burnet Institute, 3School of Health and Biomedical Sciences, RMIT University, 4Department of Infectious Diseases, Monash University

JoVE 56325


 Immunology and Infection

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University

JoVE 3884


 Bioengineering

Database-guided Flow-cytometry for Evaluation of Bone Marrow Myeloid Cell Maturation

1Department of Hematology, University Hospital of Saint-Etienne, 2Department of Immunology, University Hospital of Grenoble-Alpes, 3Department Hematology, University Hospital of Clermont-Ferrand, 4Department of Immunology, University Hospital of Nice, 5Department of Biopathology, Institute Paoli-Calmettes, 6Department of Hematology, University Hospital of Lille

Video Coming Soon

JoVE 57867


 JoVE In-Press

In Vitro Method to Control Concentrations of Halogenated Gases in Cultured Alveolar Epithelial Cells

1Department of Perioperative Medicine, CHU Clermont-Ferrand, 2Centre National de la Recherche Scientifique Unité Mixte de Recherche (CNRS UMR) 6293, Institut National de la Santé et de la Recherche Médicale (INSERM) U1103, Laboratoire de Génétique, Reproduction et Développement (GReD), Université Clermont Auvergne, 3Department of Pharmacology, CHU Clermont-Ferrand, 4Nurse Anesthetist School, CHU Clermont-Ferrand

Video Coming Soon

JoVE 58554


 JoVE In-Press

Lateral Fluid Percussion: Model of Traumatic Brain Injury in Mice

1Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, 2Spinal Cord and Brain Injury Research Center, 3Department of Anatomy and Neurobiology, Department of Physical Medicine and Rehabilitation, University of Kentucky Chandler Medical Center

JoVE 3063


 Neuroscience

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