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Magnesium Sulfate: A small colorless crystal used as an anticonvulsant, a cathartic, and an electrolyte replenisher in the treatment of pre-eclampsia and eclampsia. It causes direct inhibition of action potentials in myometrial muscle cells. Excitation and contraction are uncoupled, which decreases the frequency and force of contractions. (From Ama Drug Evaluations Annual, 1992, p1083)

Organocatalysis

JoVE 10352

Source: Vy M. Dong and Faben Cruz, Department of Chemistry, University of California, Irvine, CA

This experiment will demonstrate the concept of organocatalysis by illustrating the proper setup of a reaction that utilizes enamine catalysis. Organocatalysis is a form of catalysis that uses substoichiometric amounts of small organic molecules to accelerate reactions. This type of catalysis is complementary to other forms of catalysis such as transition metal or biocatalysis. Transition metal catalysis involves transition metals as catalysts and biocatalysis uses enzymes as catalysts. Some advantages of organocatalysis include the low toxicity and cost of the organocatalysts in comparison to many metal catalysts. In addition, most organocatalysts are not sensitive to air and moisture, unlike metal catalysts. In contrast to enzymes found in living organisms, the small molecules that act as organocatalysts are typically easy to access. Furthermore, organocatalysis offers complementary and new reactivity not observed with other forms of catalysis.


 Organic Chemistry II

Grignard Reaction

JoVE 10337

Source: Vy M. Dong and Faben Cruz, Department of Chemistry, University of California, Irvine, CA

This experiment will demonstrate how to properly carry out a Grignard reaction. The formation of an organometallic reagent will be demonstrated by synthesizing a Grignard reagent with magnesium and an alkyl halide. To demonstrate a common use of a Grignard reagent, a nucleophilic attack onto a carbonyl will be performed to generate a secondary alcohol by forming a new C-C bond.


 Organic Chemistry II

Generation and On-Demand Initiation of Acute Ictal Activity in Rodent and Human Tissue

1Division of Fundamental Neurobiology, Krembil Research Institute, 2Institute of Medical Science, Faculty of Medicine, University of Toronto, 3Institute of Biomaterials and Biomedical Engineering, University of Toronto, 4Division of Neurosurgery, Department of Surgery, University of Toronto, 5Division of Neurology, Department of Medicine, University of Toronto, 6Department of Physiology, University of Toronto

Video Coming Soon

JoVE 57952


 JoVE In-Press

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

1Institute of Chemistry L 1, Department of Biocatalysis, Technical University of Berlin, 2Institute of Chemistry PC 14, Department of Bioenergetics, Technical University of Berlin, 3Institute of Chemistry TC 7, Department of Physical Chemistry/Molecular Material Sciences, Technical University of Berlin

JoVE 57017


 Bioengineering

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

1Department of Biology, San Diego State University, 2Computational Science Research Center, San Diego State University, 3Bioinformatics and Medical Informatics Research Center, San Diego State University, 4Department of Mathematics and Statistics, San Diego State University, 5Department of Computer Science, San Diego State University, 6Mathematics and Computer Science Division, Argonne National Laboratory, 7SPARC Committee, Broad Institute

JoVE 52854


 Immunology and Infection

Unicellular Selection of Living Cells in Liquid Medium Using Laser Microdissection and a Pressure Catapulting System

1U1165, Université Paris-Diderot, INSERM, 2Pôle de Recherches Sino-Français en Science du Vivant et Génomique, Shanghai Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, 3Laboratoire de Pathologie, AP-HP-Hôpital Saint-Louis, 4Service d’Oncologie Médicale, AP-HP-Hôpital Avicenne, 5Université Paris 13

Video Coming Soon

JoVE 58234


 JoVE In-Press

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

1Department of Method Development and Analytics, Agroscope, 2Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, 3University of Basel, 4Swiss Federal Plant Protection Service, Federal Office for Agriculture, 5OptiGene Limited, 6Fera Science Limited, 7School of Natural and Environmental Sciences, Newcastle University, 8Department of Plants and Plant Products, Agroscope

Video Coming Soon

JoVE 58502


 JoVE In-Press

Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs

1Neurobiology Laboratory, National Institute of Environmental Health Sciences, 2Comparative Medicine Branch, National Institute of Environmental Health Sciences, 3Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, 4Signal Transduction Laboratory, National Institute of Environmental Health Sciences

Video Coming Soon

JoVE 58327


 JoVE In-Press

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

1Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, 2CONACYT, Facultad de Ingeniería Química, Campus de Ciencias Exactas e Ingeniería, Universidad Autónoma de Yucatán

JoVE 58166


 Biochemistry

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

1Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in energy and materials (CNPEM), 2University of Campinas (UNICAMP), 3University of Viçosa (UFV), 4CTBE, CNPEM, 5Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM), Max Planck Partner Group

JoVE 57800


 Environment

Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry

1Department of Chemistry & Biochemistry, University of Texas at Dallas, 2Undergraduate Biology, University of Texas at Dallas, 3Undergraduate Healthcare Studies, University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering, University of Texas at Dallas

JoVE 57712


 Bioengineering

Impact of Intracardiac Neurons on Cardiac Electrophysiology and Arrhythmogenesis in an Ex Vivo Langendorff System

1Department of Cardiology-Electrophysiology, cNEP (cardiac Neuro- and Electrophysiology research group), University Heart Center, University Hospital Hamburg-Eppendorf, 2DZHK (German Center for Cardiovascular Research), 3Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf

JoVE 57617


 Medicine

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

JoVE 57169


 Chemistry

A Convenient Method for Extraction and Analysis with High-Pressure Liquid Chromatography of Catecholamine Neurotransmitters and Their Metabolites

1School of Public Health of Southeast University, Laboratory of Environment and Biosafety Research Institute of Southeast University in Suzhou, 2Key Laboratory of Child Development and Learning Science (Ministry of Education), School of Biological Science & Medical Engineering, Southeast University, 3School of Public Health, Tianjin Medical University, 4British Columbia Academy, Nanjing Foreign Language School

JoVE 56445


 Chemistry

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