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Methylene Chloride: A chlorinated hydrocarbon that has been used as an inhalation anesthetic and acts as a narcotic in high concentrations. Its primary use is as a solvent in manufacturing and food technology.
 JoVE Biology

The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress

1Molecular and Cellular Physiology, Louisiana State University Health Sciences Center in Shreveport, 2Biological Sciences, Louisiana Tech University, 3Neurology, Louisiana State University Health Sciences Center in Shreveport, 4Institut Cochin, Inserm U1016, Cnrs Umr8104, Université Paris Descartes


JoVE 54632

 JoVE Immunology and Infection

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology, Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy, Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology, Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine, University of Oslo


JoVE 54615

 JoVE Environment

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

1Bioprocesses Department, Laboratory of Environmental Biotechnology, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, 2Laboratory of Molecular Biology, Escuela Superior de Medicina, Instituto Politécnico Nacional


JoVE 52956

 JoVE Bioengineering

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

1Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, 2Amnis Corporation, 3Department of Chemical and Biological Engineering, Iowa State University


JoVE 3884

 JoVE Medicine

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

1Department of Medicine B, University Hospital Münster, 2Institute of Palliative Care, University Hospital Münster, 3Biomedical Technology Center, University of Münster, 4Department of Gastroenterology, Klinikum Bielefeld


JoVE 54460

 JoVE Bioengineering

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

1South Western Sydney Clinical School, Faculty of Medicine, University of New South Wales Australia, 2School of Medicine, Western Sydney University, 3Correlative Microscopy Group, Ingham Institute for Applied Medical Research, 4Electron Microscopy Laboratory, Department of Anatomical Pathology, Sydney South West Pathology Service, New South Wales Health Pathology, 5School of Medical Sciences, Faculty of Medicine, University of New South Wales Australia


JoVE 54307

 Science Education: Essentials of Organic Chemistry

Purifying Compounds by Recrystallization

JoVE Science Education

Source: Laboratory of Dr. Jimmy Franco - Merrimack College

Recrystallization is a technique used to purify solid compounds.1 Solids tend to be more soluble in hot liquids than in cold liquids. During recrystallization, an impure solid compound is dissolved in a hot liquid until the solution is saturated, and then the liquid is allowed to cool.2 The compound should then form relatively pure crystals. Ideally, any impurities that are present will remain in the solution and will not be incorporated into the growing crystals (Figure 1). The crystals can then be removed from the solution by filtration. Not all of the compound is recoverable — some will remain in the solution and will be lost. Recrystallization is not generally thought of as a separation technique; rather, it is a purification technique in which a small amount of an impurity is removed from a compound. However, if the solubility properties of two compounds are sufficiently different, recrystallization can be used to separate them, even if they are present in nearly equal amounts. Recrystallization works best when most impurities have already been removed by another method, such as extraction or column chromatography. Fi</span>…		</p>
		
	
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 JoVE Neuroscience

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

1Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Institutet, 2Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, 3Department of Medical Biochemistry and Biophysics, Vascular Biology Unit, Karolinska Institutet


JoVE 50425

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