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Mineral Oil: A mixture of liquid hydrocarbons obtained from petroleum. It is used as laxative, lubricant, ointment base, and emollient.

Diagnostic Necropsy and Tissue Harvest

JoVE 10294

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN


Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of…

 Lab Animal Research

Surface Dye Flow Visualization: A Qualitative Method to Observe Streakline Patterns in Supersonic Flow

JoVE 10468

Flow visualization around or on a body is an important tool in aerodynamics research. It provides a method to qualitatively and quantitatively study flow structure, and it also helps researchers theorize and verify fluid flow behavior. Flow visualization can be divided into two categories: off-the-surface visualization and surface flow visualization. Off-the-surface flow…

 Aeronautical Engineering

Synthesis of an Oxygen-Carrying Cobalt(II) Complex

JoVE 10430

Source: Deepika Das, Tamara M. Powers, Department of Chemistry, Texas A&M University


Bioinorganic chemistry is the field of study that investigates the role that metals play in biology. Approximately half of all proteins contain metals and it is estimated that up to one third of all proteins rely on metal-containing active sites to…

 Inorganic Chemistry

Handling Air- and Water-Sensitive Chemicals Using a Schlenk Line

JoVE 10376

Robert M Rioux, Ajay Sathe, Zhifeng Chen, Pennsylvania State University, University Park, PA


The use of reagents sensitive to oxidation or moisture necessitates the use of air-free techniques. A Schlenk line is a routinely used glass apparatus to perform air and moisture free manipulations in a chemical laboratory1. The Schlenk line is…

 Lab Safety

DNA Methylation Analysis

JoVE 5550

Methylation at CpG dinucleotides is a chemical modification of DNA hypothesized to play important roles in regulating gene expression. In particular, the methylation of clusters of methylation sites, called “CpG islands”, near promoters and other gene regulatory elements may contribute to the stable silencing of genes, for example, during epigenetic processes…

 Genetics

Growing Crystals for X-ray Diffraction Analysis

JoVE 10216

Source: Laboratory of Dr. Jimmy Franco - Merrimack College


X-ray crystallography is a method commonly used to determine the spatial arrangement of atoms in a crystalline solid, which allows for the determination of the three-dimensional shape of a molecule or complex. Determining the three-dimensional structure of a compound is of…

 Organic Chemistry

Zebrafish Microinjection Techniques

JoVE 5130

One of the major advantages to working with zebrafish (Danio rerio) is that their genetics can be easily manipulated by microinjection of early stage embryos. Using this technique, solutions containing genetic material or knockdown constructs are delivered into the blastomeres: the embryonic cells sitting atop the yolk of the newly fertilized egg. Delivery into the cytoplasm is…

 Biology II

Drosophila Maintenance

JoVE 5084

Drosophila melanogaster, commonly known as fruit flies, are a frequently used model organism for life science research. Although starting a collection of these critters may seem as easy as leaving a banana on your kitchen counter for too long, a productive fly colony in the lab requires careful husbandry and maintenance.


This video demonstrates the necessary steps for…

 Biology I

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 2Department of Genetics and Complex Diseases, T. H. Chan School of Public Health, Harvard Medical School, 3Department of Cell Biology, Harvard Medical School, 4Sorbonne Universités, UPMC University Paris 06, 5Center for Studies in Physics and Biology, The Rockefeller University

JoVE 56086

 Biology
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