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Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

1Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, 2Eck Institute for Global Health, University of Notre Dame, 3Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, 4INRS-Institut Armand-Frappier, 5Department of Biology, Indiana University, 6Department of Biological Sciences, University of Notre Dame

JoVE 52338


 Biology

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

1Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology, SUNY Upstate Medical University, 4Feist-Weiller Cancer Center, Louisiana State University Health Sciences Center

JoVE 4165


 Immunology and Infection

Establishment of Larval Zebrafish as an Animal Model to Investigate Trypanosoma cruzi Motility In Vivo

1Laboratory of Neurosciences and Circadian Rhythms, School of Medicine, Universidad de los Andes, 2Biophysics Group, Department of Physics, Universidad de los Andes, 3Laboratory of Basic Medical Sciences, School of Medicine, Universidad de los Andes, 4Department of Microbiology and Immunology, University of North Carolina, 5Notre Dame Initiative for Global Development, University of Notre Dame, 6USAID Research and Innovation Fellowship program

JoVE 56238


 Developmental Biology

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

1Biomedical Engineering Department, Cornell University, 2Neurosurgical Laboratory for Translational Stem Cell Research, Weill Cornell Brain Tumor Center, Weill Cornell Medical College of Cornell University, 3Cell Morphology Department, Instituto de Investigacion Principe Felipe, 4Department of Chemical and Biomolecular Engineering, Cornell University

JoVE 674


 Biology

An Introduction to Cell Motility and Migration

JoVE 5643

Cell motility and migration play important roles in both normal biology and in disease. On one hand, migration allows cells to generate complex tissues and organs during development, but on the other hand, the same mechanisms are used by tumor cells to move and spread in a process known as cancer metastasis. One of the primary cellular machineries that make cell movement possible is an intracellular network of myosin and actin molecules, together known as “actomyosin”, which creates a contractile force to pull a cell in different directions.In this video, JoVE presents a historical overview of the field of cell migration, noting how early work on muscle contraction led to the discovery of the actomyosin apparatus. We then explore some of the questions researchers are still asking about cell motility, and review techniques used to study different aspects of this phenomenon. Finally, we look at how researchers are currently studying cell migration, for example, to better understand metastasis.


 Cell Biology

A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

1Department of Biochemistry, Stanford University School of Medicine, 2Department of Mechanical and Aerospace Engineering, University of California San Diego, 3Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute, Stanford University School of Medicine

JoVE 57361


 Immunology and Infection

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Szent Istvan University

JoVE 56835


 Developmental Biology

Caenorhabditis Sieve: A Low-tech Instrument and Methodology for Sorting Small Multicellular Organisms

1Department of Biology and Wildlife, University of Alaska Fairbanks, 2Institute of Arctic Biology, University of Alaska Fairbanks, 3Department of Chemistry and Biochemistry, University of Alaska Fairbanks, 4Departments of Biology and Chemistry, Earlham College, 5Department of Biological Sciences, College of Natural Science and Mathematics, California State University Long Beach

JoVE 58014


 Developmental Biology

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

1Department of Animal Reproduction, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine, Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science, University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5Veterinary Services, L'Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013

JoVE 55237


 Developmental Biology

Imaging Cell Interaction in Tracheal Mucosa During Influenza Virus Infection Using Two-Photon Intravital Microscopy

1Faculty of Biomedical Sciences, Institute for Research in Biomedicine, Università della Svizzera italiana (USI), 2Graduate School of Cellular and Molecular Sciences, Faculty of Medicine, University of Bern, 3Institute of Computational Science, Università della Svizzera italiana (USI)

Video Coming Soon

JoVE 58355


 JoVE In-Press

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

1Department of Neurosurgery, UCLA David Geffen School of Medicine, 2Department of Molecular and Medical Pharmacology, UCLA David Geffen School of Medicine, 3Department of Medicine, UCLA David Geffen School of Medicine, 4Brain Research Institute, UCLA David Geffen School of Medicine, 5Jonsson Comprehensive Cancer Center, UCLA David Geffen School of Medicine

JoVE 52416


 Immunology and Infection

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud

JoVE 56337


 Cancer Research

Ex Vivo Imaging of Resident CD8 T Lymphocytes in Human Lung Tumor Slices Using Confocal Microscopy

1Institut Cochin, Inserm U1016-CNRS UMR8104, Université Paris Descartes, 2Department of Pathology, Paris Centre University Hospitals, Université Paris Descartes, 3INSERM U1138, Cancer and Immune Escape, Cordeliers Research Center, University Pierre and Marie Curie, 4Department of Thoracic Surgery, Paris Centre University Hospitals, University Paris Descartes

JoVE 55709


 Cancer Research

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