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Multiprotein Complexes: Macromolecular complexes formed from the association of defined protein subunits.

Anatomy of Chloroplasts

JoVE 10750

Green algae and plants, including green stems and unripe fruit, harbor chloroplasts—the vital organelles where photosynthesis takes place. In plants, the highest density of chloroplasts is found in the mesophyll cells of leaves.

A double membrane surrounds chloroplasts. The outer membrane faces the cytoplasm of the plant cell on one side and the intermembrane space of the chloroplast on the other. The inner membrane separates the narrow intermembrane space from the aqueous interior of the chloroplast, called the stroma. Within the stroma, another set of membranes form disk-shaped compartments—known as thylakoids. The interior of a thylakoid is called the thylakoid lumen. In most plant species, the thylakoids are interconnected and form stacks called grana. Embedded in the thylakoid membranes are multi-protein light-harvesting (or antenna) complexes. These complexes consist of proteins and pigments, such as chlorophyll, that capture light energy to perform the light-dependent reactions of photosynthesis. These processes release oxygen and produce chemical energy in the form of ATP and NADPH. The second part of photosynthesis—the Calvin cycle—is light-independent and takes place in the stroma of the chloroplast. The Calvin cycle captures CO2 and uses the ATP and NADPH to ultimately produce sugar.

 Core: Photosynthesis

Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation

1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

JoVE 60029

 Biochemistry

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy

JoVE 56981

 Biology

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

1Institute of Physiology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 2Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 3Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany, 4Logopharm GmbH, Germany

JoVE 60096

 Biochemistry

CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia

JoVE 53597

 Chemistry
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