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Myosin-Light-Chain Kinase: An enzyme that phosphorylates myosin light chains in the presence of Atp to yield myosin-light chain phosphate and Adp, and requires calcium and Calmodulin. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

1Harris-Wellbeing Preterm Birth Research Centre, Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, 2School of Biomedical Sciences, The University of Queensland, 3Faculty of Chemistry, Institute of Biological Chemistry, University of Vienna, 4Institute for Molecular Bioscience, University of Queensland, 5Center for Physiology and Pharmacology, Medical University of Vienna

JoVE 56639


 Medicine

A Multi-well Format Polyacrylamide-based Assay for Studying the Effect of Extracellular Matrix Stiffness on the Bacterial Infection of Adherent Cells

1Department of Biochemistry, Stanford University School of Medicine, 2Department of Mechanical and Aerospace Engineering, University of California San Diego, 3Departments of Biochemistry, Microbiology and Immunology and Howard Hughes Medical Institute, Stanford University School of Medicine

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JoVE 57361


 JoVE In-Press

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