Show Advanced Search

REFINE YOUR SEARCH:

Containing Text
- - -
+
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by journal

Filter by science education

 
 
Nuclear Envelope: The membrane system of the Cell nucleus that surrounds the nucleoplasm. It consists of two concentric membranes separated by the perinuclear space. The structures of the envelope where it opens to the cytoplasm are called the nuclear pores (Nuclear pore).

Identification of Critical Conditions for Immunostaining in the Pea Aphid Embryos: Increasing Tissue Permeability and Decreasing Background Staining

1Department of Entomology, National Taiwan University, 2Institute of Biotechnology, National Taiwan University, 3Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 4Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica

JoVE 53883


 Developmental Biology

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, 5Department of Microbiology and Immunology, The Medical College of Wisconsin, 6Department of Pediatrics, The Medical College of Wisconsin, 7Department of Medicine, The Medical College of Wisconsin

Video Coming Soon

JoVE 55063


 JoVE In-Press

Mammalian Cell Division in 3D Matrices Via Quantitative Confocal Reflection Microscopy

1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine

Video Coming Soon

JoVE 56364


 JoVE In-Press

Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes

1Center for AIDS Health Disparities Research, Meharry Medical College, 2Department of Biochemistry and Cancer Biology, Meharry Medical College, 3School of Graduate Studies and Research, Meharry Medical College, 4Department of Microbiology and Immunology, Meharry Medical College, 5Tennessee Center for AIDS Research (TN-CFAR), Meharry Medical College

JoVE 54581


 Immunology and Infection

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

1Center for Molecular Oncology, Barts Cancer Institute, Queen Mary University of London, 2Sino-British Research Centre for Molecular Oncology, National Center for International Research in Cell and Gene Therapy, Zhengzhou University, 3School of Basic Medical Sciences, Academy of Medical Sciences, Zhengzhou University

JoVE 54171


 Immunology and Infection

Fabrication of a Functionalized Magnetic Bacterial Nanocellulose with Iron Oxide Nanoparticles

1Department of Bioengineering, University of Illinois at Urbana-Champaign, 2Department of Nuclear, Plasma and Radiological Engineering, University of Illinois at Urbana-Champaign, 3Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, 4Program of Study and Control of Tropical Diseases (PECET), University of Antioquia, 5Sealy Center for Vaccine Development, University of Texas Medical Branch, 6WHO Collaborating Center for Vaccine Research, Evaluation and Training on Emerging Infectious Diseases, University of Texas Medical Branch, 7Beckman Institute, University of Illinois at Urbana-Champaign

JoVE 52951


 Bioengineering

Neuronal Transfection Methods

JoVE 5215

Transfection - the process of transferring genetic material into cells - is a powerful tool for the rapid and efficient manipulation of gene expression in cells. Because this method can be used to silence the expression of specific proteins or to drive the expression of foreign or modified proteins, transfection is an extremely useful tool in the study of the cellular and molecular processes that govern neuron function. However, mature neurons have a number of properties that make them difficult to transfect, so specialized techniques are required for the genetic manipulation of this cell type. This video reviews the principles and rationale behind transfecting neurons. Three common strategies for neuronal transfection are discussed, including nucleofection, gene-gun, and viral transduction. In addition to describing how each of these techniques overcomes the challenges associated with transfecting neurons, the presentation includes a description of how all three methods are performed. Finally, several applications of neuronal transfection are introduced, such as the expression of fluorescent tubulin proteins to visualize neuron morphology, and selective gene silencing to generate a cell culture model of Parkinson’s disease.


 Neuroscience

Results below contain some, but not all of your search terms.
Results below contain some, but not all of your search terms.

Interictal High Frequency Oscillations Detected with Simultaneous Magnetoencephalography and Electroencephalography as Biomarker of Pediatric Epilepsy

1Fetal-Neonatal Neuroimaging and Developmental Science Center, Division of Newborn Medicine, Department of Medicine, Boston Children's Hospital, Harvard Medical School, 2Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, 3Division of Epilepsy Surgery, Department of Neurosurgery, Boston Children's Hospital, Harvard Medical School, 4Division of Epilepsy and Clinical Neurophysiology, Department of Neurology, Boston Children's Hospital, Harvard Medical School

JoVE 54883


 Medicine

Results below contain some, but not all of your search terms.

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

1Developmental and Stem Cell Biology, Hospital for Sick Children, 2Children's Health Research Institute, University of Western Ontario, 3Department of Physiology and Pharmacology, University of Western Ontario, 4Neurosciences and Mental Health, Hospital for Sick Children, 5Department of Paediatrics, University of Western Ontario

JoVE 51526


 Developmental Biology

Results below contain some, but not all of your search terms.
12345678928
More Results...