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Open Reading Frames: A sequence of successive nucleotide triplets that are read as Codons specifying Amino acids and begin with an Initiator codon and end with a stop codon (Codon, Terminator).
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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

1Multi-Organ Transplant Program, University Health Network, 2Institute of Medical Science, University of Toronto, 3Divison of Neurosurgery, University Health Network, 4Division of Cardiac Surgery, University Health Network

JoVE 54543


 Immunology and Infection

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Multi-analyte Biochip (MAB) Based on All-solid-state Ion-selective Electrodes (ASSISE) for Physiological Research

1Department of Agricultural and Biological Engineering, Birck-Bindley Physiological Sensing Facility, Purdue University, 2NASA Ames Research Center, 3Department of Chemistry, Pennsylvania State University Hazleton, 4Cooley LLP, 5NASA Life and Physical Sciences, Human Exploration and Operations Mission Directorate, NASA Headquarters

JoVE 50020


 Bioengineering

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Newton's Laws of Motion

JoVE 10038

Source: Andrew Duffy, PhD, Department of Physics, Boston University, Boston, MA

This experiment examines at various situations involving two interacting objects.

First, the experiment examines the forces that two objects apply to one another while they collide. The objects are wheeled carts that have variable masses. The purpose of this experiment is to discover when the force the first cart exerts on the other is the same magnitude as the force the second cart exerts back on the first, as well as when these two forces have different magnitudes. Second, it examines the forces that two objects exert on one another when one cart is pushing or pulling the second one. Again, the focus is on exploring the situations in which the two forces have the same magnitude and in which they have different magnitudes.


 Physics I

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Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy

1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group, German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology, Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4), Robert Koch Institute

JoVE 56666


 Biology

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Acrylic Resin Molding Based Head Fixation Technique in Rodents

1Department of Anatomy, School of Medicine and Brain Science and Engineering Institute, Kyungpook National University, 2Department of Pharmacology, School of Dentistry and Brain Science and Engineering Institute, Kyungpook National University, 3Department of Pharmacology, School of Medicine and Brain Science and Engineering Institute, Kyungpook National University

JoVE 53064


 Neuroscience

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Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

1Department of Microbiology, Biochemistry, Immunology, Morehouse School of Medicine, 2Department of Medicine, Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 4Yerkes National Primate Research Center

JoVE 53495


 Immunology and Infection

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Soil Nutrient Analysis: Nitrogen, Phosphorus, and Potassium

JoVE 10077

Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University

In this experiment, three soil macronutrients are chemically extracted, combined with color-based reagents, then analyzed using color to determine the nutrient concentration present in the soil sample.

Nitrogen, phosphorus, and potassium are the main components of soil fertilizer. These methods isolate each nutrient from the soil into a solution that can be analyzed using turbidity and color to determine the concentration of nutrients present in the soil sample. Knowing present concentration informs environmental scientists of a nutrient deficiency or surplus in soils used to support plant production, and also provides general insight into basic biogeochemical cycles of an ecosystem.


 Environmental Science

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Essential Metal Uptake in Gram-Negative Bacteria: X-Ray Fluorescence, Radioisotopes, and Cell Fractionation

1Graduate Biomedical Sciences Microbiology Theme, University of Alabama at Birmingham, 2Department of Radiology, University of Alabama at Birmingham, 3Office of the Provost, University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology, University of Alabama at Birmingham

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JoVE 57169


 JoVE In-Press

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Method of Standard Addition

JoVE 10201

Source: Laboratory of Dr. Paul Bower - Purdue University

The method of standard additions is a quantitative analysis method, which is often used when the sample of interest has multiple components that result in matrix effects, where the additional components may either reduce or enhance the analyte absorbance signal. That results in significant errors in the analysis results. Standard additions are commonly used to eliminate matrix effects from a measurement, since it is assumed that the matrix affects all of the solutions equally. Additionally, it is used to correct for the chemical phase separations performed in the extraction process. The method is performed by reading the experimental (in this case fluorescent) intensity of the unknown solution and then by measuring the intensity of the unknown with varying amounts of known standard added. The data are plotted as fluorescence intensity vs. the amount of the standard added (the unknown itself, with no standard added, is plotted ON the y-axis). The least squares line intersects the x-axis at the negative of the concentration of the unknown, as shown in Figure 1. Figure 1Results below contain some, but not all of your search terms.

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