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Paraffin Embedding: The infiltrating of tissue specimens with paraffin, as a supporting substance, to prepare for sectioning with a microtome.

Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules

1UMR 1188 Diabète athérothombose Thérapie Réunion Océan Indien (DéTROI), Saint-Denis de La Réunion, France, Université de La Réunion, INSERM, 2RIPA (Radiochimie et Imagerie du Petit Animal), Cyclotron de la Réunion Océan Indien CYROI, 3CHU de La Réunion

JoVE 55203


 Developmental Biology

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 2Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea

JoVE 55988


 Developmental Biology

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

1Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute, 3Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute

JoVE 55095


 Medicine

Protocol for HER2 FISH Using a Non-cross-linking, Formalin-free Tissue Fixative to Combine Advantages of Cryo-preservation and Formalin Fixation

1Christian Doppler Laboratory for Biospecimen Research and Biobanking Technologies, Institute of Pathology, Medical University Graz, 2Institute of Pathology, Medical University Graz, 3Research and Development, Qiagen GmbH

Video Coming Soon

JoVE 55885


 JoVE In-Press

Utilizing 3D Printing Technology to Merge MRI with Histology: A Protocol for Brain Sectioning

1Translational Neuroradiology Section, National Institute of Neurological Disorders and Stroke, 2Cerebral Microcirculation Section, National Institute of Neurological Disorders and Stroke, 3Viral Immunology Section, National Institute of Neurological Disorders and Stroke

JoVE 54780


 Neuroscience

Comparable Decellularization of Fetal and Adult Cardiac Tissue Explants As 3D-like Platforms for in Vitro Studies

1i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 2INEB - Instituto de Engenharia Biomédica, Universidade do Porto, 3Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Universidade do Porto, 4Gladstone Institutes, University of California San Francisco, 5Faculty of Medicine, University of Porto

Video Coming Soon

JoVE 56924


 JoVE In-Press

Human Lung Dendritic Cells: Spatial Distribution and Phenotypic Identification in Endobronchial Biopsies Using Immunohistochemistry and Flow Cytometry

1Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet, 2Division of Medicine, Department of Public Health and Clinical Medicine, Umeå University, 3Divison of Infectious Diseases, Department of Clinical Microbiology, Umeå University

JoVE 55222


 Immunology and Infection

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

1Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine, Karolinska Institutet, 2Department of Neuroimmunology, Center for Brain Research, Medical University of Vienna, 3Department of Medical Biochemistry and Biophysics, Vascular Biology Unit, Karolinska Institutet

JoVE 50425


 Neuroscience

Studying Diabetes Through the Eyes of a Fish: Microdissection, Visualization, and Analysis of the Adult tg(fli:EGFP) Zebrafish Retinal Vasculature

1Department of Vascular Biology and Tumorangiogenesis, Center for Biomedicine and Medical Technology Mannheim (CBTM), Medical Faculty Mannheim, Heidelberg University, 2V. Medical Clinic, Medical Faculty Mannheim, Heidelberg University

Video Coming Soon

JoVE 56674


 JoVE In-Press

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

1Bionics Institute, 2Department of Anatomical Pathology, St Vincent's Hospital Melbourne, 3Department of Pathology, University of Melbourne, 4Medical Bionics Department, University of Melbourne

JoVE 50411


 Medicine

Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

1Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 2School of Medicine, University of Milan, 3School of Biology, University of Pavia, 4Division of Breast Surgery, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, 5Division of Medical Oncology, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, 6Department of Biomedical, Surgical, and Dental Sciences, University of Milan

JoVE 56686


 Immunology and Infection

Reconstruction of 3-Dimensional Histology Volume and its Application to Study Mouse Mammary Glands

1Department of Medical Biophysics, University of Toronto, 2Platform Biological Sciences, Sunnybrook Research Institute, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Physical Sciences, Sunnybrook Research Institute, 5Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 6Manitoba Institute of Cell Biology, University of Manitoba

JoVE 51325


 Biology

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-Cut Lung Slices

1Fraunhofer Institute for Toxicology and Experimental Medicine, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 2Institute for Pathology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH)

Video Coming Soon

JoVE 57042


 JoVE In-Press

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

1Department Tissue Engineering and Regenerative Medicine, University Hospital Wuerzburg, 2Translational Center Regenerative Therapies (TLC-RT), Fraunhofer Institute for Silicate Research ISC, 3Fujifilm Manufacturing Europe B.V.

Video Coming Soon

JoVE 57363


 JoVE In-Press

Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics

1Department of Pathology, Memorial Sloan Kettering Cancer Center, 2Molecular Pharmacology and Chemistry Program, Memorial Sloan Kettering Cancer Center, 3Department of Radiology, Weill Cornell Medical College, 4Department of Medicine, Memorial Sloan Kettering Cancer Center, 5Department of Oncology, Memorial Sloan Kettering Cancer Center, 6Department of Surgery, Memorial Sloan Kettering Cancer Center

JoVE 52157


 Medicine

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