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Perchloric Acid: An oxidizing agent that is used in Analytical chemistry techniques for separation of potassium from sodium. (From Merck Index, 11th ed)

Safe Handling of Mineral Acids

JoVE 10370

Source: Robert M. Rioux & Taslima A. Zaman, Pennsylvania State University, University Park, PA

A mineral acid (or inorganic acid) is defined as a water-soluble acid derived from inorganic minerals by chemical reaction as opposed to organic acids (e.g. acetic acid, formic acid). Examples of mineral acids include: • Boric acid (CAS No.10043-35-3) • Chromic acid (CAS No.1333-82-0) • Hydrochloric acid (CAS No.7647-01-0) • Hydrofluoric acid (CAS No. 7664-39-3) • Nitric acid (CAS No. 7697-37-2) • Perchloric acid (CAS No. 7601-90-3) • Phosphoric acid (CAS No.7664-38-2) • Sulfuric acid (CAS No.7664-93-9) Mineral acids are commonly found in research laboratories and their corrosive nature makes them a significant safety risk. Since they are important reagents in the research laboratory and often do not have substitutes, it is important that they are handled properly and with care. Some acids are even shock sensitive and under certain conditions may cause explosions (i.e., salts of perchloric acid).


 Lab Safety

Fume Hoods and Laminar Flow Cabinets

JoVE 10372

Robert M. Rioux & William A. Elliott, Pennsylvania State University, University Park, PA

Fume hoods and laminar flow cabinets are engineering controls that operate under similar principles. Both use a constant flow of air to prevent contamination of the laboratory environment and its inhabitants. Fume hoods prevent hazardous substances from exiting the hood workspace, whereas laminar flow cabinets prevent contaminants from entering the cabinet workspace. Fume hoods are ventilation systems designed to minimize exposure to hazardous vapors, fumes, and particles. A constant flow of air is drawn into the hood opening, limiting the escape of vapors, fumes, and particles, and then is pulled out through the exhaust. Laminar flow cabinets are used to maintain a sterile/clean environment by constantly flowing high-efficiency particulate arrestance (HEPA)-filtered air outwards, minimizing contaminated air entering the cabinet workspace. The HEPA-filtered air reduces the opportunity for harmful chemicals or particles from entering the laboratory. A HEPA filter removes 99.97% or greater of 0.3 µm particles.


 Lab Safety

Examination of Rapid Dopamine Dynamics with Fast Scan Cyclic Voltammetry During Intra-oral Tastant Administration in Awake Rats

1Interdepartmental Neuroscience Program, Yale University, 2Department of Biotechnical and Clinical Laboratory Sciences, School of Medicine and Biomedical Sciences, University at Buffalo, 3Department of Psychiatry, Yale School of Medicine, 4Department of Cellular and Molecular Physiology, Yale School of Medicine

JoVE 52468


 Behavior

Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake

1Molecular Neuropharmacology and Genetics Laboratory, Lundbeck Foundation Center for Biomembranes in Nanomedicine, Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2Laboratory of Neuropsychiatry, Psychiatric Center Copenhagen and Department of Neuroscience and Pharmacology, University of Copenhagen

JoVE 56093


 Neuroscience

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

1Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 2College of Veterinary Medicine, Hunan Agricultural University, 3Key Laboratory of Chemical Engineering Process and Technology for High-efficiency Conversion, College of Chemistry and Material Sciences, Heilongjiang University, 4Department of Obstetrics and Gynecology, Wayne State University School of Medicine

JoVE 58219


 Bioengineering

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

1Department of Chemical and Biomolecular Engineering, Ohio University, 2Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, 3Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School

JoVE 51023


 Bioengineering

Application of Retinoic Acid to Obtain Osteocytes Cultures from Primary Mouse Osteoblasts

1Renal Research Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 2Department of Nephrology, Dialysis and Renal Transplant, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 3Renal Physiopathology Laboratory, Department of Medical, Surgical and Health Sciences, University of Trieste

JoVE 51465


 Biology

Synthesis and Characterization of Fe-doped Aluminosilicate Nanotubes with Enhanced Electron Conductive Properties

1Department of Applied Science and Technology, Politecnico di Torino, 2Department of Civil and Mechanical Engineering, Università degli Studi di Cassino e del Lazio Meridionale, 3Institute of Chemistry, Politecnico di Torino, 4Department of Chemistry & NIS Interdepartmental Centre, University of Turin, 5INSTM Unit of Torino-Politecnico, Politecnico di Torino

JoVE 54758


 Chemistry

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