Show Advanced Search

REFINE YOUR SEARCH:

Containing Text
- - -
+
Filter by author or institution
GO
Filter by publication date
From:
October, 2006
Until:
Today
Filter by journal section

Filter by science education

 
 
Poly A: A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

1College of Chemistry and Molecular Engineering, Peking University, 2Beijing National Laboratory for Molecular Sciences, Peking University, 3Peking-Tsinghua Center for Life Sciences, Peking University, 4Synthetic and Functional Biomolecules Center, Peking University, 5Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University

JoVE 58580


 Biochemistry

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

1Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 2Department of Medical Oncology, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, 3Unit of Biostatistics and Clinical Trials, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS

Video Coming Soon

JoVE 58615


 JoVE In-Press

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

1David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 2Department of Respiratory Medicine, Graduate School of Medicine, The University of Tokyo, 3Hastings Center for Pulmonary Research, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, Keck School of Medicine, University of Southern California, 4Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo

JoVE 57577


 Biology

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

JoVE 56004


 Biochemistry

Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR

1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

JoVE 55505


 Genetics

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

JoVE 55135


 Immunology and Infection

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins

1Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, Rockefeller University, 2Berlin Institute for Medical Systems Biology, Max-Delbrück-Center for Molecular Medicine, 3Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 4Biozentrum der Universität Basel and Swiss Institute of Bioinformatics (SIB), 5Genomics Resource Center, Rockefeller University

JoVE 2034


 Biology

Production and Targeting of Monovalent Quantum Dots

1Department of Otolaryngology, University of California, San Francisco, 2Department of Chemistry, University of California, Berkeley, 3Materials Science Division, Lawrence Berkeley National Laboratory, 4Department of Pharmaceutical Chemistry, University of California, San Francisco, 5Tetrad Graduate Program, University of California, San Francisco, 6Center for Systems and Synthetic Biology, University of California, San Francisco, 7Chemistry and Chemical Biology Graduate Program, University of California, San Francisco

JoVE 52198


 Bioengineering

RNA-Seq

JoVE 5548

Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown transcripts.Here, JoVE presents the basic principles behind RNA-seq. We then discuss the experimental and analytical steps of a general RNA-seq protocol. Finally, we examine how researchers are currently using RNA-seq, for example, to compare gene expression between different biological samples, or to characterize protein-RNA interactions.


 Genetics

12
More Results...