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Primitive Streak: A linear band of rapidly proliferating cells that begins near the posterior end of an embryo and grows cranially. Primitive streak is formed during Gastrulation by the convergent migration of primary ectodermal cells (Epiblast). The knot at the tip of the streak is called Hensen node.

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

1Graduate Program in Pharmacology and Experimental Therapeutics, University of Cologne, 2Department of Dermatology and Venereology, University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne

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JoVE 58321


 JoVE In-Press

Development of the Chick

JoVE 5155

The chicken embryo (Gallus gallus domesticus) provides an economical and accessible model for developmental biology research. Chicks develop rapidly and are amenable to genetic and physiological manipulations, allowing researchers to investigate developmental pathways down to the cell and molecular levels.

This video review of chick development begins by describing the process of egg fertilization and formation within the chicken reproductive tract. Next, the most commonly used chick staging nomenclature, the Hamburger Hamilton staging series, is introduced. Major events in chick development are then outlined, including the dramatic cellular movements known as gastrulation that form the three major cell layers: The ectoderm, mesoderm, and endoderm. Cells from these layers go on to generate all the tissues within the organism, as well as extraembryonic membranes, which are necessary for the transport of gases, nutrients, and wastes within the eggshell. To conclude the discussion, some exciting techniques will be presented as strategies for studying chick development in greater detail.


 Biology II

Evaluation of the Cell Invasion and Migration Process: A Comparison of the Video Microscope-based Scratch Wound Assay and the Boyden Chamber Assay

1UMR CNRS 5822 /IN2P3, IPNL, PRISME, Laboratoire de Radiobiologie Cellulaire et Moléculaire, Faculté de Médecine Lyon-Sud, Université Lyon 1, 2Département de Radiothérapie, Institut de Cancérologie de la Loire - Lucien Neuwirth, 3Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud

JoVE 56337


 Cancer Research

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