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Purple Membrane: Functionally and structurally differentiated, purple-pigmented regions of the cytoplasmic membrane of some strains of Halobacterium halobium. The membrane develops under anaerobic conditions and is made almost entirely of the purple pigment Bacteriorhodopsins. (From Singleton & Sainsbury Dictionary of Microbiology and Molecular Biology, 2d ed)

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

1Developmental and Stem Cell Biology, Hospital for Sick Children, 2Children's Health Research Institute, University of Western Ontario, 3Department of Physiology and Pharmacology, University of Western Ontario, 4Neurosciences and Mental Health, Hospital for Sick Children, 5Department of Paediatrics, University of Western Ontario

JoVE 51526


 Developmental Biology

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

1Division of Urologic Surgery, Washington University in St. Louis School of Medicine, 2Department of Urology, University of Wisconsin School of Medicine and Public Health, 3Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, 4O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health

JoVE 53837


 Biology

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital, Harvard Medical School, 2College of Life Sciences, Jilin University, 3Faculty of Health Sciences, University of Macau

JoVE 56100


 Developmental Biology

Preparation of Giant Vesicles Exhibiting Visible-light-induced Morphological Changes

1Department of Applied Chemistry, School of Applied Science, National Defense Academy of Japan, 2Department of Applied Physics, School of Applied Science, National Defense Academy of Japan, 3Department of Materials Science and Technology, Faculty of Engineering, Niigata University

Video Coming Soon

JoVE 54817


 JoVE In-Press

Techniques for Processing Eyes Implanted With a Retinal Prosthesis for Localized Histopathological Analysis

1Bionics Institute, 2Department of Anatomical Pathology, St Vincent's Hospital Melbourne, 3Department of Pathology, University of Melbourne, 4Medical Bionics Department, University of Melbourne

JoVE 50411


 Medicine

Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes

1Department of Neurosurgery, UCLA David Geffen School of Medicine, 2Department of Molecular and Medical Pharmacology, UCLA David Geffen School of Medicine, 3Department of Medicine, UCLA David Geffen School of Medicine, 4Brain Research Institute, UCLA David Geffen School of Medicine, 5Jonsson Comprehensive Cancer Center, UCLA David Geffen School of Medicine

JoVE 52416


 Immunology and Infection

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

1Department of Immunology, Reims University Hospitals, Robert Debré Hospital, 2Faculty of Medicine, LRN EA 4682, University of Reims Champagne-Ardenne, 3Department of Internal Medicine and Geriatrics, Reims University Hospitals, Maison Blanche Hospital, 4Faculty of Medicine, EA 3797, University of Reims Champagne-Ardenne

JoVE 56012


 Genetics

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine

JoVE 55561


 Biochemistry

Preparation of Light-responsive Membranes by a Combined Surface Grafting and Postmodification Process

1Laboratory for Protection and Physiology, Empa, Swiss Federal Laboratories for Materials Science and Technology, 2Laboratory of Advanced Fibers, Empa, Swiss Federal Laboratories for Materials Science and Technology, 3Division of Neonatology, University Hospital Zurich

JoVE 51680


 Bioengineering

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