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Reducing Agents: Materials that add an electron to an element or compound, that is, decrease the positiveness of its valence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)

Reducing Agents

JoVE 10354

Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA


Controlling the reactivity and selectivity during the synthesis of a molecule is very important criteria for chemists. This has led to the development of many reagents that allow chemists to pick and choose reagents suitable for a given task.…


 Organic Chemistry II

Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

1Plant Molecular and Cellular Biology Program, University of Florida, 2Department of Biology, University of Florida, 3Genetics Institute (UFGI), University of Florida, 4Alkali Soil Natural Environmental Science Center, Northeast Forestry University, 5Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida

JoVE 57422


 Biology

Application of Elemental Lanthanides in the Selective C-F Activation of Trifluoromethylated Benzofulvenes Providing Access to Various Difluoroalkenes

1Institut de Chimie Moléculaire de Reims UMR 7312, Université de Reims Champagne Ardenne, 2Institut Charles Gerhardt Montpellier UMR 5253, Ecole Nationale Supérieure de Chimie de Montpellier

JoVE 57948


 Chemistry

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

1Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, 2School of Integrative Engineering, Chung-Ang University, 3Division of Synthetic Biology and Regenerative Medicine, Institute for Quantitative Health Science and Engineering, Michigan State University

JoVE 57384


 Bioengineering

Chemical Storage: Categories, Hazards And Compatibilities

JoVE 10380

Source: Robert M. Rioux & Taslima A. Zaman, Pennsylvania State University, University Park, PA


While the use of various chemicals in experimental research is essential, it is also important to safely store and maintain them as a part of the Environmental, Health and Safety (EHS) program. The properties of chemicals and …


 Lab Safety

Two-Dimensional Gel Electrophoresis

JoVE 5686

Two-dimensional gel electrophoresis (2DGE) is a technique that can resolve thousands of biomolecules from a mixture. This technique involves two distinct separation methods that have been coupled together: isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This physically separates compounds across two axes of a gel by their isoelectric points…


 Biochemistry

Cell-surface Biotinylation Assay

JoVE 5647

A cell can regulate the amount of particular proteins on its cell membrane through endocytosis, following which cell surface proteins are effectively sequestered in the cytoplasm. Once within a cell, these surface proteins can be either destroyed or “recycled” back to the membrane. The cell surface biotinylation assay provides researchers with a way to study…


 Cell Biology

Separating Protein with SDS-PAGE

JoVE 5058

Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis, or SDS-PAGE, is a widely-used technique for separating mixtures of proteins based on their size and nothing else. SDS, an anionic detergent, is used to produce an even charge across the length of proteins that have been linearized. By first loading them into a gel made of polyacrylamide and then applying an electric field to the gel,…


 Basic Methods in Cellular and Molecular Biology

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