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Risk Assessment: The qualitative or quantitative estimation of the likelihood of adverse effects that may result from exposure to specified health hazards or from the absence of beneficial influences. (Last, Dictionary of Epidemiology, 1988)

16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species

JoVE 10510

Source: Ewa Bukowska-Faniband1, Tilde Andersson1, Rolf Lood1
1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund, Sweden


Planet Earth is a habitat for millions of bacterial species, each of which has specific characteristics. Identification of bacterial species is…

 Microbiology

RNA Analysis of Environmental Samples Using RT-PCR

JoVE 10104

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Bradley Schmitz


Reverse transcription-polymerase chain reaction (RT-PCR) involves the same process as conventional PCR — cycling temperature to amplify nucleic acids. However, while conventional PCR only amplifies…

 Environmental Microbiology

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School

JoVE 57042

 Immunology and Infection

Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

1Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, 2Institute of Pharmacology and Toxicology, Department of Veterinary Medicine, Freie Universität Berlin, 3German Federal Institute for Risk Assessment (BfR), 4Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine

JoVE 57046

 Behavior

Using Retinal Imaging to Study Dementia

1Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, 2Department of Medicine & Therapeutics, The Chinese University of Hong Kong, 3Therese Pei Fong Chow Research Centre for Prevention of Dementia, The Chinese University of Hong Kong, 4Gerald Choa Neuroscience Centre, The Chinese University of Hong Kong, 5Memory Aging and Cognition Centre, National University Health System, 6Department of Pharmacology, National University of Singapore, 7Singapore Eye Research Institute, Singapore National Eye Centre, 8Duke-NUS Medical School, National University of Singapore

JoVE 56137

 Medicine

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

1Infectious Diseases Group, Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering, National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore

JoVE 58829

 Immunology and Infection

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

1Department of Hematology, VU University Medical Center, 2Pediatric Oncology/Hematology, VU University Medical Center, 3Janssen Research & Development, LLC, 4Princess Máxima Center for Pediatric Oncology

JoVE 56386

 Cancer Research

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture

1Department of Medicine Solna, Center for Molecular Medicine, Karolinska University Hospital, Karolinska Institutet, 2Section of Intercellular Interactions, Eunice Shriver National Institute of Child Health and Human Development, National Institutes of Health

JoVE 57013

 Immunology and Infection

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

1Aurora Research Institute, Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science, University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine, Johns Hopkins University

JoVE 59906

 Bioengineering

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

1Division of Respirology, Departments of Medicine and Immunology, Toronto Lung Transplant Program, Multiorgan Transplant Program, Toronto General Research Institute, University of Toronto and University Health Network, 2Latner Thoracic Surgery Laboratories, Toronto General Research Institute, University Health Network, 3National Institutes of Health Research, Oxford Biomedical Research Centre, Translational Immunology Laboratory, NDORMS, Kennedy Institute of Rheumatology, University of Oxford, 4Transplantation Research Immunology Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital, University of Oxford

JoVE 55283

 Immunology and Infection

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

1Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), Université de Bordeaux, 2Centre d'Etudes Nucléaires Bordeaux Gradignan (CENBG), CNRS, 3Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), CNRS, 4Institut de Chimie de la Matière Condens é e de Bordeaux (ICMCB), Université de Bordeaux

JoVE 55041

 Chemistry

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

1Centre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, 2Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, 3Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, 4Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services, Australian National University Medical School, 5Infection Management Services, Australian National University Medical School, 6Department of Microbiology and Infectious Diseases, St. Vincent's Hospital, 7Department of Infectious Diseases, Peter MacCallum Cancer Centre

JoVE 56714

 Medicine

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

1Focal Area Infection Biology, Biozentrum, University of Basel, 2Centre d’Immunologie de Marseille-Luminy, Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical, Universidad de Navarra, 4BioDataAnalysis GmbH

JoVE 54263

 Immunology and Infection

Using Tg(Vtg1:mcherry) Zebrafish Embryos to Test the Estrogenic Effects of Endocrine Disrupting Compounds

1Department of Aquaculture, Institute of Aquaculture and Environmental Safety, Faculty of Agricultural and Environmental Sciences, Szent István University, 2Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham

Video Coming Soon

JoVE 60462

 JoVE In-Press

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2The Michael J. Fox Foundation for Parkinson's Research, 3Department of Medical and Molecular Genetics, Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee

Video Coming Soon

JoVE 58956

 JoVE In-Press

Technical Modification of the Terminal Ureter During Total Transperitoneal Laparoscopic Nephroureterectomy for Upper Urinary Tract Urothelial Carcinoma

1(Urology) Beijing Hospital, National Center of Gerontology, The Joint Unit of the Chinese Academy of Medical Sciences, 2Graduate School of Peking Union Medical College, Beijing Hospital

JoVE 60662

 Medicine

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow

Video Coming Soon

JoVE 60460

 JoVE In-Press

Prospecting Microbial Strains for Bioremediation and Probiotics Development for Metaorganism Research and Preservation

1LEMM, Laboratory of Molecular Microbial Ecology, Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro (UFRJ), 2Genome Center, University of California, Davis, 3IMAM-AquaRio - Rio de Janeiro Aquarium Research Center

JoVE 60238

 Bioengineering

Integrating Augmented Reality Tools in Breast Cancer Related Lymphedema Prognostication and Diagnosis

1Physical and Rehabilitative Medicine, Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", 2Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 3Division of Breast Surgery, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 4Division of Medical Oncology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, 5Division of Pathology, Maggiore della Carità Hospital, 6Department of Health Sciences, University of Eastern Piedmont "A. Avogadro", 7Department of Biomedical, Surgical, and Dental Sciences, University of Milan

JoVE 60093

 Medicine

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr

JoVE 59755

 Genetics
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