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Sodium Bicarbonate: A white, crystalline powder that is commonly used as a pH buffering agent, an electrolyte replenisher, systemic alkalizer and in topical cleansing solutions.

Esterification - Student Protocol

JoVE 11220

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Esterification

In this lab, you will synthesize an ester from a carboxylic acid and an alcohol in the presence of sulfuric acid. This reaction is called Fischer esterification. The sulfuric acid makes the carboxylic acid more reactive towards the alcohol. …

 Lab: Chemistry

Extraction - Student Protocol

JoVE 11199

Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA



Cellulose Recovery

Extraction and filtration can separate compounds based on their solubility properties. In this lab, you'll separate a mixture of cellulose, caffeine, and benzoic acid based on their solubilities in…

 Lab: Chemistry

Photosynthesis - Prep Student

JoVE 10623

Plant Pigment Chromatography and Floating Leaf Discs in a Vacuum
On the day before the experiment, fill a container with water.
Place the spinach leaves into the water and make sure they are submerged.
Leave the spinach leaves to soak overnight at room temperature.
Wearing gloves, prepare and measure out 18 cm x 304 cm sections of…

 Lab Bio

Extraction - Prep Student

JoVE 11197

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Preparation of the Laboratory

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




Put on a lab coat, splash-proof safety glasses, and…

 Lab: Chemistry

Esterification - Prep Student

JoVE 11218

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Preparation of the Laboratory

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




First, put on a lab coat, safety glasses, and nitrile…

 Lab: Chemistry

Basic Organic Chemistry Techniques - Prep Student

JoVE 11185

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Preparation of the Laboratory

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




To set up for this lab experiment, wear the appropriate…

 Lab: Chemistry

Cleaning Glassware

JoVE 10342

Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA


Organic synthesis is about transforming a readily available reagent into a more valuable product. Having clean glassware is crucial for the efficiency of this process. Dirty glassware can potentially affect the reaction and make isolation of…

 Organic Chemistry II

Safe Handling of Mineral Acids

JoVE 10370

Source: Robert M. Rioux & Taslima A. Zaman, Pennsylvania State University, University Park, PA


A mineral acid (or inorganic acid) is defined as a water-soluble acid derived from inorganic minerals by chemical reaction as opposed to organic acids (e.g. acetic acid, formic acid). Examples of mineral acids…

 Lab Safety

Proper Lab Notebook Keeping - Prep Student

JoVE 11182

Source: Lara Al Hariri at the University of Massachusetts Amherst, MA, USA



Preparation of the Laboratory

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.




Put on a lab coat, splash-proof safety glasses, and…

 Lab: Chemistry

Creating a Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte to Study the Role of Cellular Architecture in Cardiomyocyte Systems Biology

1Cell Structure and Mechanobiology Group, University of Melbourne, 2Systems Biology Laboratory, Melbourne School of Engineering, University of Melbourne, 3Department of Biomedical Engineering, University of Melbourne, 4School of Mathematics and Statistics, Faculty of Science, University of Melbourne, 5Department of Engineering Science, University of Auckland, 6Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, 7ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of Melbourne, 8School of Medicine, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, 9Living Systems Institute, University of Exeter

JoVE 56817

 Bioengineering

Generation and On-Demand Initiation of Acute Ictal Activity in Rodent and Human Tissue

1Division of Fundamental Neurobiology, Krembil Research Institute, 2Institute of Medical Science, Faculty of Medicine, University of Toronto, 3Institute of Biomaterials and Biomedical Engineering, University of Toronto, 4Division of Neurosurgery, Department of Surgery, University of Toronto, 5Division of Neurology, Department of Medicine, University of Toronto, 6Department of Physiology, University of Toronto

JoVE 57952

 Neuroscience

Mammalian Cell Division in 3D Matrices via Quantitative Confocal Reflection Microscopy

1Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 2Johns Hopkins Physical Sciences - Oncology Center, Johns Hopkins University, 3Department of Biomedical Engineering, Johns Hopkins University, 4Departments of Oncology and Pathology and Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine

JoVE 56364

 Bioengineering

Fluorescence Biomembrane Force Probe: Concurrent Quantitation of Receptor-ligand Kinetics and Binding-induced Intracellular Signaling on a Single Cell

1Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Biosciences, Georgia Institute of Technology, 2Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 3Charles Perkins Centre, The University of Sydney, 4Institute of Biophysics, Laboratory of RNA Biology, Chinese Academy of Sciences, 5University of Chinese Academy of Sciences, 6School of Medicine and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University

JoVE 52975

 Bioengineering

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin

JoVE 52010

 Biology

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

1Division of Physical Therapy, Department of Orthopedics & Rehabilitation, University of New Mexico, 2Department of Ecosystem Science and Management, University of Wyoming, 3School of Pharmacy, University of Wyoming, 4Department of Environmental and Radiological Health Sciences, Colorado State University, 5Center for Environmental Medicine, Colorado State University, 6College of Pharmacy, California Northstate University

JoVE 52715

 Environment

Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System Development

1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience, University of Barcelona

JoVE 59481

 Neuroscience

High-throughput Detection Method for Influenza Virus

1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, 2Department of Microbiology, Mount Sinai School of Medicine, 3Laboratory of Molecular Genetics, Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin, 6Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

JoVE 3623

 Immunology and Infection

An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes

1Laboratory of Mitochondrial Biology and Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, 2Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health

JoVE 54918

 Immunology and Infection

Monitoring Astrocyte Reactivity and Proliferation in Vitro Under Ischemic-Like Conditions

1Department of Neuroscience, School of Medicine, Universidad Central del Caribe, 2Department of Biochemistry, School of Medicine, Universidad Central del Caribe, 3Department of Pharmacology and Toxicology, Medical Sciences, Campus, University of Puerto Rico

JoVE 55108

 Neuroscience

Characterizing Multiscale Mechanical Properties of Brain Tissue Using Atomic Force Microscopy, Impact Indentation, and Rheometry

1Department of Materials Science and Engineering, Massachusetts Institute of Technology, 2Department of Biological Engineering, Massachusetts Institute of Technology, 3Department of Mechanical Engineering, Massachusetts Institute of Technology, 4Department of Neurology, The F.M. Kirby Neurobiology Center, Boston Children’s Hospital, Harvard Medical School

JoVE 54201

 Neuroscience
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