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Stainless Steel: Stainless steel. A steel containing Ni, Cr, or both. It does not tarnish on exposure and is used in corrosive environments. (Grant & Hack's Chemical Dictionary, 5th ed)

Tensile Strength of Resorbable Biomaterials

JoVE 10471

Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut

For over 4000 years, sutures have been used as a medical intervention. The earliest records indicate linen was the biomaterial of choice. Catgut, which is still in use today, was reportedly used to …

 Biomedical Engineering

Analysis of Thermal Expansion via Dilatometry

JoVE 10490

Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT

Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a…

 Materials Engineering

Nanocrystalline Alloys and Nano-grain Size Stability

JoVE 10484

Source: Sina Shahbazmohamadi and Peiman Shahbeigi-Roodposhti-Roodposhti, School of Engineering, University of Connecticut, Storrs, CT

Alloys with grain size less than 100 nm are known as nanocrystaline alloys. Due to their enhanced physical and mechanical properties, there is an ever-increasing demand to employ them in various industries…

 Materials Engineering

Proper Use of Autoclaves

JoVE 10381

Robert M. Rioux & Zhifeng Chen, Pennsylvania State University, University Park, PA

Autoclaving is one of the most commonly used methods in the laboratory for the purpose of decontamination. The most common items decontaminated with an autoclave are those that contact biological samples (typically those containing microorganisms). An…

 Lab Safety

Enthalpy of Reaction - Prep Student

JoVE 11155

Source: Smaa Koraym at Johns Hopkins University, MD, USA

Preparation of 2.0 M Hydrochloric Acid

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.

To set up for this lab experiment, wear the appropriate personal…

 Lab: Chemistry

Ideal Gas Law - Prep Student

JoVE 11146

Source: Smaa Koraym at Johns Hopkins University, MD, USA

Preparation of the Laboratory

Here, we show the laboratory preparation for 10 students working in pairs, with some excess. Please adjust quantities as needed.

Put on a lab coat, splash-proof safety glasses or goggles, and appropriate…

 Lab: Chemistry

Compound Administration II

JoVE 10388

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

Compound administration is often an integral component of an animal study. Many factors need to be evaluated to ensure that the compound is delivered correctly. The route of administration affects the mechanisms of…

 Lab Animal Research

Cleaning Glassware

JoVE 10342

Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA

Organic synthesis is about transforming a readily available reagent into a more valuable product. Having clean glassware is crucial for the efficiency of this process. Dirty glassware can potentially affect the reaction and make isolation of…

 Organic Chemistry II

Considerations for Rodent Surgery

JoVE 10285

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN

The Guide for the Care and Use of Laboratory Animals1 dictates that rodent survival surgery be performed aseptically. Aseptic technique utilizes specific practices that minimize the contamination of the surgical site, including…

 Lab Animal Research

Isolation of Fecal Bacteria from Water Samples by Filtration

JoVE 10213

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Luisa Ikner

The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events…

 Environmental Microbiology

Hydraulic Jumps

JoVE 10405

Source: Alexander S Rattner and Mahdi Nabil; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA

When liquid flows along an open channel at high velocity, the flow can become unstable, and slight disturbances can cause the liquid upper surface to transition abruptly to a higher level…

 Mechanical Engineering

Operation of High-pressure Reactor Vessels

JoVE 10400

Robert M Rioux, Pennsylvania State University, University Park, PA

The use of gases in a synthetic chemistry laboratory is essential for carrying out a variety of highly facile and atom economical transformations. Reactions such as hydrogenation, oxidation, and amination require the use of gases like hydrogen, oxygen, and ammonia. Due to…

 Lab Safety

Working with Centrifuges

JoVE 10365

Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA

Earth's gravitational force is capable of separating heterogeneous mixtures. However, many systems avert this type of separation owing to the length of time usually required in such cases. Centrifugation is one of the most powerful tools for …

 Lab Safety

Glovebox and Impurity Sensors

JoVE 10317

Source: Tamara M. Powers, Department of Chemistry, Texas A&M University 

The glovebox provides a straightforward means to handle air- and moisture-sensitive solids and liquids. The glovebox is what it sounds like: a box with gloves attached to one or more sides, which allows the user to perform manipulations within the glovebox under an inert…

 Inorganic Chemistry

Conducting Reactions Below Room Temperature

JoVE 10224

Source: Laboratory of Dr. Dana Lashley - College of William and Mary

Demonstration by: Matt Smith

When new bonds are formed in the course of a chemical reaction, it requires that the involved species (atoms or molecules) come in very close proximity and collide into one another. The collisions between…

 Organic Chemistry

Ideal Gas Law

JoVE 5537

Source: Laboratory of Dr. Andreas Züttel - Swiss Federal Laboratories for Materials Science and Technology

The ideal gas law describes the behavior of most common gases at near-ambient conditions and the tendency of all chemical matter in the dilute limit. It is a fundamental relationship between three measurable macroscopic system…

 General Chemistry

An Introduction to Working in the Hood

JoVE 5036

Hoods are a common class of instrument, which act as a protective enclosure for various types of laboratory experiments. They serve to shield experimental samples from the environment, as well as to protect the researcher from hazardous or infectious samples. “The Hood” is a term that can include laminar flow benches, fume hoods, as well as tissue culture…

 General Laboratory Techniques

Bovine Mammary Gland Biopsy Techniques

1National Animal Nutrition Program, a National Research Support Project (NRSP-9), Department of Animal and Food Sciences, University of Kentucky, 2School of Performing Arts, Virginia Tech, 3Department of Large Animal Clinical Sciences, The Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 4Department of Dairy Science, Virginia Tech, 5Department of Animal Biosciences, University of Guelph, 6School of Visual Arts, Virginia Tech

JoVE 58602


Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

1Department of Anesthesiology and Critical Care, Soroka Medical Center, Ben-Gurion University of the Negev, 2Department of Anesthesiology, Yale University School of Medicine, 3Department of Neurosurgery, Soroka University Medical Center and the Faculty of Health Sciences, Ben-Gurion University of the Negev, 4Department of Physiology, Faculty of Biology, Ecology and Medicine, Dnepropetrovsk State University

JoVE 61309

 JoVE In-Press

Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles

1Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, 2Silvio O. Conte Center for Oxytocin and Social Cognition, Center for Translational Social Neuroscience, Department of Psychiatry and Behavioral Sciences, Yerkes National Primate Research Center, Emory University, 3Escuela Nacional de Estudios Superiores Juriquilla, Universidad Nacional Autónoma de México, 4Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología

JoVE 61402


Arterial Pouch Microsurgical Bifurcation Aneurysm Model in the Rabbit

1Department of Neurosurgery, Kantonsspital Aarau, 2Cerebrovascular Research Group, Department for BioMedical Research, University of Bern, 3Division of Neuroradiology, Department of Radiology, Kantonsspital Aarau, 4Department of Neurosurgery, Neurocenter and Regenerative Neuroscience Cluster, Inselspital, Bern University Hospital, University of Bern, 5Department for Biomedical Research, Faculty of Medicine, University of Bern

JoVE 61157


Ammonia Fiber Expansion (AFEX) Pretreatment of Lignocellulosic Biomass

1Department of Chemical and Biochemical Engineering, Rutgers-State University of New Jersey, 2Michigan Biotechnology Institute (MBI), 3Department of Chemical Engineering and Materials Science, Michigan State University, 4Engineering Technology Department, Biotechnology Program, College of Technology, University of Houston, 5Department of Chemical Engineering, Michigan Technological University

JoVE 57488


Sucrose Preference and Novelty-Induced Hypophagia Tests in Rats using an Automated Food Intake Monitoring System

1Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine, Charité-Universitätsmedizin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, 2Department of Internal Medicine, Helios Clinic, 3Department of Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, 4Department of Quantitative Health Sciences, University of Massachusetts Medical School

JoVE 60953


Translaminar Autonomous System Model for the Modulation of Intraocular and Intracranial Pressure in Human Donor Posterior Segments

1North Texas Eye Research Institute, Department of Pharmacology and Neuroscience, University of North Texas Health Science Center, 2Mechanical Engineer Consultant, 3Department of Ophthalmology and Visual Sciences, School of Medicine and Public Health, University of Wisconsin

JoVE 61006


Electrochemotherapy in 3D Ocular Melanoma Spheroids using a Customized Electrode

1Department of Ophthalmology, University Hospital Halle (Saale), Martin-Luther University Halle-Wittenberg, 2Department of Ophthalmology, University Hospital Essen, Duisburg Essen University, 3Department of Ophthalmology, Saarland University Medical Center, 4Liverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool

JoVE 60611

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