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Stainless Steel: Stainless steel. A steel containing Ni, Cr, or both. It does not tarnish on exposure and is used in corrosive environments. (Grant & Hack's Chemical Dictionary, 5th ed)

Tensile Strength of Resorbable Biomaterials

JoVE 10471

Source: Peiman Shahbeigi-Roodposhti and Sina Shahbazmohamadi, Biomedical Engineering Department, University of Connecticut, Storrs, Connecticut


For over 4000 years, sutures have been used as a medical intervention. The earliest records indicate linen was the biomaterial of choice. Catgut, which is still in use today, was reportedly used to …

 Biomedical Engineering

Analysis of Thermal Expansion via Dilatometry

JoVE 10490

Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT


Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a…

 Materials Engineering

Nanocrystalline Alloys and Nano-grain Size Stability

JoVE 10484

Source: Sina Shahbazmohamadi and Peiman Shahbeigi-Roodposhti-Roodposhti, School of Engineering, University of Connecticut, Storrs, CT


Alloys with grain size less than 100 nm are known as nanocrystaline alloys. Due to their enhanced physical and mechanical properties, there is an ever-increasing demand to employ them in various industries…

 Materials Engineering

Proper Use of Autoclaves

JoVE 10381

Robert M. Rioux & Zhifeng Chen, Pennsylvania State University, University Park, PA


Autoclaving is one of the most commonly used methods in the laboratory for the purpose of decontamination. The most common items decontaminated with an autoclave are those that contact biological samples (typically those containing microorganisms). An…

 Lab Safety

Compound Administration II

JoVE 10388

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN



Compound administration is often an integral component of an animal study. Many factors need to be evaluated to ensure that the compound is delivered correctly. The route of administration affects the mechanisms of…

 Lab Animal Research

Cleaning Glassware

JoVE 10342

Source: Vy M. Dong and Daniel Kim, Department of Chemistry, University of California, Irvine, CA


Organic synthesis is about transforming a readily available reagent into a more valuable product. Having clean glassware is crucial for the efficiency of this process. Dirty glassware can potentially affect the reaction and make isolation of…

 Organic Chemistry II

Considerations for Rodent Surgery

JoVE 10285

Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN


The Guide for the Care and Use of Laboratory Animals1 dictates that rodent survival surgery be performed aseptically. Aseptic technique utilizes specific practices that minimize the contamination of the surgical site, including…

 Lab Animal Research

Isolation of Fecal Bacteria from Water Samples by Filtration

JoVE 10213

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - Arizona University
Demonstrating Author: Luisa Ikner


The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events…

 Environmental Microbiology

Hydraulic Jumps

JoVE 10405

Source: Alexander S Rattner and Mahdi Nabil; Department of Mechanical and Nuclear Engineering, The Pennsylvania State University, University Park, PA


When liquid flows along an open channel at high velocity, the flow can become unstable, and slight disturbances can cause the liquid upper surface to transition abruptly to a higher level…

 Mechanical Engineering

Operation of High-pressure Reactor Vessels

JoVE 10400

Robert M Rioux, Pennsylvania State University, University Park, PA


The use of gases in a synthetic chemistry laboratory is essential for carrying out a variety of highly facile and atom economical transformations. Reactions such as hydrogenation, oxidation, and amination require the use of gases like hydrogen, oxygen, and ammonia. Due to…

 Lab Safety

Working with Centrifuges

JoVE 10365

Source: Robert M. Rioux & Suprita Jharimune, Pennsylvania State University, University Park, PA


Earth's gravitational force is capable of separating heterogeneous mixtures. However, many systems avert this type of separation owing to the length of time usually required in such cases. Centrifugation is one of the most powerful tools for …

 Lab Safety

Glovebox and Impurity Sensors

JoVE 10317

Source: Tamara M. Powers, Department of Chemistry, Texas A&M University 


The glovebox provides a straightforward means to handle air- and moisture-sensitive solids and liquids. The glovebox is what it sounds like: a box with gloves attached to one or more sides, which allows the user to perform manipulations within the glovebox under an inert…

 Inorganic Chemistry

Conducting Reactions Below Room Temperature

JoVE 10224

Source: Laboratory of Dr. Dana Lashley - College of William and Mary


Demonstration by: Matt Smith


When new bonds are formed in the course of a chemical reaction, it requires that the involved species (atoms or molecules) come in very close proximity and collide into one another. The collisions between…

 Organic Chemistry

Ideal Gas Law

JoVE 5537

Source: Laboratory of Dr. Andreas Züttel - Swiss Federal Laboratories for Materials Science and Technology


The ideal gas law describes the behavior of most common gases at near-ambient conditions and the tendency of all chemical matter in the dilute limit. It is a fundamental relationship between three measurable macroscopic system…

 General Chemistry

An Introduction to Working in the Hood

JoVE 5036

Hoods are a common class of instrument, which act as a protective enclosure for various types of laboratory experiments. They serve to shield experimental samples from the environment, as well as to protect the researcher from hazardous or infectious samples. “The Hood” is a term that can include laminar flow benches, fume hoods, as well as tissue culture…

 General Laboratory Techniques

Bovine Mammary Gland Biopsy Techniques

1National Animal Nutrition Program, a National Research Support Project (NRSP-9), Department of Animal and Food Sciences, University of Kentucky, 2School of Performing Arts, Virginia Tech, 3Department of Large Animal Clinical Sciences, The Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 4Department of Dairy Science, Virginia Tech, 5Department of Animal Biosciences, University of Guelph, 6School of Visual Arts, Virginia Tech

JoVE 58602

 Biology

Functionalization and Dispersion of Carbon Nanomaterials Using an Environmentally Friendly Ultrasonicated Ozonolysis Process

1Aerospace Division, Defence Science and Technology Group, 2Maritime Division, Defence Science and Technology Group, 3Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, 4Department of Mechanical Engineering, University of Delaware, 5Department of Materials Science and Engineering, University of Delaware, 6Center for Composite Materials, University of Delaware

JoVE 55614

 Environment

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

1Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology, Indiana University School of Medicine

JoVE 3188

 Medicine

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

1Department of Bioengineering & Therapeutic Sciences, University of California, San Francisco, 2Department of Obstetrics, Gynecology & Reproductive Sciences, University of California, San Francisco, 3Keck School of Medicine, University of Southern California

JoVE 51745

 Bioengineering

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

1Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group, Eindhoven University of Technology, 2Institute for Molecules and Materials, Radboud University, 3Institute of Bioengineering, School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)

JoVE 59655

 Bioengineering

Data Acquisition and Analysis In Brainstem Evoked Response Audiometry In Mice

1Experimental Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices, 2KBRwyle GmbH, 3Cognitive Neurophysiology, Department of Psychiatry and Psychotherapy, Faculty of Medicine, University of Cologne, 4Molecular and Cellular Cognition, German Center for Neurodegenerative Diseases (DZNE), 5Institute of Neurophysiology, Faculty of Medicine, University of Cologne, 6Federal Institute for Drugs and Medical Devices (BfArM), 7Thescon GmbH

JoVE 59200

 Neuroscience

Measurement of Maximum Isometric Force Generated by Permeabilized Skeletal Muscle Fibers

1Department of Orthopaedic Surgery, University of Michigan Medical School, 2Department of Molecular & Integrative Physiology, University of Michigan Medical School, 3Department of Biomedical Engineering, University of Michigan Medical School, 4Department of Surgery, Section of Plastic Surgery, University of Michigan Medical School

JoVE 52695

 Bioengineering
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