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Transition Temperature: The temperature at which a substance changes from one state or conformation of matter to another.

## Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

1Laboratory for Biomaterials and Bioengineering, Department Min-Met-Materials Eng & CHU de Québec Research Center, Canada Research Chair I for the Innovation in Surgery, Laval University, 2NSERC CREATE Program for Regenerative Medicine (NCPRM), Laval University, 3Department Electronics, Information and Bioengineering, Politecnico di Milano, 4Department of Chemical and Materials Engineering, University of Alberta, 5National Institute for Nanotechnology, National Research Council (Canada), 6Department of Chemical and Biochemical Engineering, University of Western Ontario

## A New Approach that Eliminates Handling for Studying Aggression and the "Loser" Effect in Drosophila melanogaster

1Department of Neurobiology, Harvard Medical School

## Determining Rate Laws and the Order of Reaction

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry

All chemical reactions have a specific rate defining the progress of reactants going to products. This rate can be influenced by temperature, concentration, and the physical properties of the reactants. The rate also includes the intermediates and transition states that are formed but are neither the reactant nor the product. The rate law defines the role of each reactant in a reaction and can be used to mathematically model the time required for a reaction to proceed. The general form of a rate equation is shown below:     where A and B are concentrations of different molecular species, m and n are reaction orders, and k is the rate constant. The rate of nearly every reaction changes over time as reactants are depleted, making effective collisions less likely to occur. The rate constant, however, is fixed for any single reaction at a given temperature. The reaction order illustrates the number of molecular species involved in a reaction. It is very important to know the rate law, including rate constant and reaction order, which can only be deter

## A Murine Orthotopic Bladder Tumor Model and Tumor Detection System

1Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, 2Department of Urology, National University Hospital

## Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

1Laboratory of Genetics, University of Wisconsin, 2Department of Neurobiology, University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program, University of Pittsburgh School of Medicine

## Epitaxial Growth of Perovskite Strontium Titanate on Germanium via Atomic Layer Deposition

1McKetta Department of Chemical Engineering, The University of Texas at Austin

## A Protocol of Manual Tests to Measure Sensation and Pain in Humans

1Chronic Pain Research Consortium, Duquesne University, 2Department of Physical Therapy, Duquesne University, 3Department of Biological Sciences, Duquesne University, 4Department of Psychology, Duquesne University, 5Department of Occupational Therapy, Duquesne University

## Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

1Department of Microbiology and Immunology, University at Buffalo, State University of New York, 2Witebsky Center for Microbial Pathogenesis and Immunology, University at Buffalo, State University of New York, 3New York State Center of Excellence in Bioinformatics and Life Sciences, University at Buffalo, State University of New York

## The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

1Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne

## Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors

1Department of Transdisciplinary Studies, Graduate School of Convergence Science and Technology, Seoul National University, 2Center for Functional Connectomics, Korea Institute of Science and Technology, 3College of Life Sciences and Biotechnology, Korea University, 4Advanced Institutes of Convergence Technology

## Fractional Distillation

Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut

Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation is used when the liquid is (a) relatively pure (containing no more than 10% liquid contaminants), (b) has a non-volatile component, such as a solid contaminant, or (c) is mixed with another liquid with a boiling point that differs by at least 25 °C. Fractional distillation is used when separating mixtures of liquids whose boiling points are more similar (separated by less than 25 °C). This video will detail the fractional distillation of a mixture of two common organic solvents, cyclohexane and toluene.

## Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

1Department of Biomedical Engineering, Oregon Health and Science University, 2Knight Cancer Institute, Oregon Health and Science University, 3OHSU Center for Spatial Systems Biomedicine, Oregon Health and Science University

## Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

1Department of Pediatric Oncology/Hematology, VU University Medical Center, 2Department of Hematology, VU University Medical Center, 3Department of Medical Oncology, VU University Medical Center, 4Department of Clinical Genetics, VU University Medical Center, 5Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, 6Amsterdam Immunology and Rheumatology Center, VU University Medical Center, 7Princess Máxima Center for Pediatric Oncology, 8Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, 9Institute of Nanoscience and Nanotechnology, CNR-Nano

## The Synthesis of [Sn10(Si(SiMe3)3)4]2- Using a Metastable Sn(I) Halide Solution Synthesized via a Co-condensation Technique

1Chemistry Department, Institute of Inorganic Chemistry, University of Tübingen

## Kinematic History of a Salient-recess Junction Explored through a Combined Approach of Field Data and Analog Sandbox Modeling

1Department of Earth and Environmental Science, Franklin and Marshall College, 2Department of Geosciences, University of Massachusetts, Amherst

## Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

1Department of Biology, University of Alabama at Birmingham, 2Nutrition Métabolisme Aquaculture, INRA UR1067, 3Laboratoire de Physiologie et Genomique des Poissons, INRA UR1037

## Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

1R&D Reagents, Miltenyi Biotec GmbH, 2In vivo Tumorbiology, Oncotest GmbH

## Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

1University of Zürich & Zürich-Basel Plant Science Center

## The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation

1Department of Anatomy and Cell Biology, University of Iowa Carver College of Medicine

## Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

1Department of Biochemistry and Molecular Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University

## Seeded Synthesis of CdSe/CdS Rod and Tetrapod Nanocrystals

1Department of Chemical Engineering, UC Berkeley, 2Department of Materials Science and Engineering, UC Berkeley, 3Department of Chemistry, UC Berkeley, 4Materials Sciences Division, Lawrence Berkeley National Laboratory, 5Department of Chemistry, University of Chicago, 6Center for Nanoscale Materials, Argonne National Laboratory

## Fabrication of Gate-tunable Graphene Devices for Scanning Tunneling Microscopy Studies with Coulomb Impurities

1Department of Physics, University of California at Berkeley, 2Department of Chemistry, University of California at Berkeley, 3Department of Chemical and Biomolecular Engineering, University of California at Berkeley, 4National Institute for Materials Science (Japan), 5Materials Sciences Division, Lawrence Berkeley National Laboratory, 6Kavli Energy NanoSciences Institute, University of California at Berkeley and Lawrence Berkeley National Laboratory

## Primary Outcome Assessment in a Pig Model of Acute Myocardial Infarction

1Department of Experimental Cardiology, University Medical Center Utrecht, 2Department of Cardiology, University Medical Center Utrecht, 3Department of Clinical Chemistry and Hematology, University Medical Center Utrecht, 4Interuniversity Cardiology Institutes of the Netherlands (ICIN)

## Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method

1School of Materials Science and Engineering, Nanyang Technological University, 2Centre for Biomimetic Sensor Science, Nanyang Technological University, 3School of Chemical and Biomedical Engineering, Nanyang Technological University

## The Use of High-resolution Infrared Thermography (HRIT) for the Study of Ice Nucleation and Ice Propagation in Plants

1U.S Department of Agriculture, Agricultural Research Service (USDA-ARS), Kearneysville, WV, 2Institute of Botany, University of Innsbruck, 3Department of Plant Science, University of Saskatechewan

## Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

1Department of Molecular Genetics, The Ohio State University

## Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

1Department of Biology, Ball State University, 2Bioproduct Research & Development, Eli Lilly and Company

## Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

1Department of Physics, Tokyo University of Science, 2Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University

## Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala

1Department of Pharmacology, Medical University of Innsbruck, 2Hertie Institute for Clinical Brain Research and Centre for Integrative Neuroscience, University of Tübingen

## CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 2Institute of Chemistry, Proteomics Unit, Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry, University of Brasilia

## Colorimetric Detection of Bacteria Using Litmus Test

1Department of Biochemistry and Biomedical Sciences, McMaster University, 2Department of Chemistry and Chemical Biology, McMaster University, 3Department of Medicine, McMaster University

## Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

1Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, 2Department of Medicine, Section of Gastroenterology and Hepatology, Baylor College of Medicine

## A Fluorescence-based Assay of Phospholipid Scramblase Activity

1Department of Biochemistry, Weill Cornell Medical College

## Quantifying Fish Swimming Behavior in Response to Acute Exposure of Aqueous Copper Using Computer Assisted Video and Digital Image Analysis

1Columbia Environmental Research Center, US Geological Survey, 2US Geological Survey

## Fabrication of a Dipole-assisted Solid Phase Extraction Microchip for Trace Metal Analysis in Water Samples

1Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, 2Center for Measurement Standards, Industrial Technology Research Institute, 3National Synchrotron Radiation Research Center, 4Department of Chemistry, National Changhua University of Education

## Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

1Department of Chemistry and Biochemistry, University of Denver, 2Magnetic Imaging Group, Applied Physics Division, Physical Measurements Laboratory, National Institute of Standards and Technology, 3Department of Radiology, Geisel School of Medicine, Dartmouth University, 4Department of Biochemistry, West Virginia University, 5Department of Electrical and Computer Engineering, University of Denver, 6Department of Engineering, University of Denver

## Identification Of Erythromyeloid Progenitors And Their Progeny In The Mouse Embryo By Flow Cytometry

1Department of Developmental and Stem Cell Biology, CNRS UMR3738, Department of Immunology, Institut Pasteur, 2Cellule Pasteur UPMC, University Pierre et Marie Curie

## Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

1Department of Physics, National Taiwan University, 2Department of Applied Physics, Osaka University, 3Molecular Imaging Center, National Taiwan University

## Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

1Department of Molecular and Medical Pharmacology, University of California, Los Angeles (UCLA), 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles (UCLA)

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