Ecole Normale Superieure de Lyon 3 articles published in JoVE Developmental Biology A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse Carla Guzman-Moreno*1, Peizhao Zhang*2, Olivia R. Phillips*1, Mathias Block*3, Benjamin J. Glennon*1, Meghan Holbrook2, Lauren Weigand2, Cecilia W. Lo1, Jiuann-Huey I. Lin1,4 1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Département de Biologie, École Normale Supérieure de Lyon, 4Department of Critical Care Medicine, University of Pittsburgh This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction. Genetics Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae Diedre Reitz1, Jérôme Savocco2, Aurèle Piazza2, Wolf-Dietrich Heyer1,3 1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis The D-loop capture (DLC) and D-loop extension (DLE) assays utilize the principle of proximity ligation together with quantitative PCR to quantify D-loop formation, D-loop extension, and product formation at the site of an inducible double-stranded break in Saccharomyces cerevisiae. Biology The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells Pierre Dourlen1,2, Clemence Levet1, Alexandre Mejat1, Alexis Gambis3, Bertrand Mollereau1 1Laboratory of Molecular Biology of the Cell, Ecole Normale Supérieure de Lyon, 2INSERM U744, Institut Pasteur de Lille, Université Lille-Nord de France, 3Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer, The Rockefeller University The Tomato/GFP-FLP/FRT method involves visualizing mosaic photoreceptor cells in living Drosophila. It can be used to follow individual photoreceptor cell fates in the retina for days or weeks. This method is ideal for studies of retinal degeneration and neurodegenerative diseases or photoreceptor cell development.
