Erasmus Medical Center 2 articles published in JoVE Developmental Biology Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing Naomi J. M. Kakiailatu1, Laura E. Kuil1,2, Eric Bindels3, Joke T. M. Zink3, Michael Vermeulen3, Veerle Melotte4, Maria M. Alves1 1 Here, we describe a method for gut isolation from zebrafish larvae at 5 days post fertilization, for single-cell RNA sequencing analysis. Bioengineering A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue Hanneke Crielaard1, Su Guvenir Torun1, Tamar B. Wissing1,2, Pablo de Miguel Muñoz1,3, Gert-Jan Kremers4, Frank J. H. Gijsen1,3, Kim Van Der Heiden1,2, Ali C. Akyildiz1,3 1Department of Biomedical Engineering, Erasmus Medical Center, 2Department of Biomedical Engineering, Eindhoven University of Technology, 3Department of Biomechanical Engineering, Delft University of Technology, 4Erasmus Optical Imaging Center, Erasmus Medical Center We have developed a mechano-imaging pipeline to study the heterogeneous structural and mechanical atherosclerotic plaque properties. This pipeline enables correlation of the local predominant angle and dispersion of collagen fiber orientation, the rupture behavior, and the strain fingerprints of the fibrous plaque tissue.
