Benemerita Universidad Autonoma de Puebla 3 articles published in JoVE Neuroscience Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin Valeria Piazza1, Milvia Alata1, Victor H. Hernandez2, José R. Eguibar3,4, Carmen Cortes3 1Centro de Investigaciones en Óptica A.C. (CIO), 2Division of Sciences and Engineering, Department of Chemical, Electronic, and Biomedical Engineering, Universidad de Guanajuato, 3Institute of Physiology, Benemérita Universidad Autónoma de Puebla, 4Dirección General de Desarrollo Internacional, Benemérita Universidad Autónoma de Puebla In this article, we present a protocol to detect microtubule-loaded oligodendrocytes in a model of tubulinopathy through a simple, innovative second harmonic generation microscopy approach. Behavior A Laboratory Method to Measure Contagious Yawning in Rats Alejandro Moyaho1, Alejandra P. Díaz-Loyo1, Oscar E. Juárez-Mora1, Evelyn Beristain-Castillo2 1Laboratorio de Ecología de la Conducta, Instituto de Fisiología, Benemérita Universídad Autónoma de Puebla Pue. Mexico, 2Facultad de Medicina, Benemérita Universidad Autónoma de Puebla Pue. Mexico The method described here aims to obtain yawn contagion curves in pairs of familiar or unfamiliar male rats. Cages with holes separated by either clear or opaque partitions with (or without) holes are used to detect whether visual, olfactory, or both types of sensory cues can stimulate yawn contagion. Chemistry Syntheses, Crystallization, and Spectroscopic Characterization of 3,5-Lutidine N-Oxide Dehydrate Rosario Merino-García1, Samuel Hernández-Anzaldo1, Yasmi Reyes-Ortega1 1Centro de Química, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla Herein, we report the synthesis and crystallization of 3,5-lutidine N-oxide dehydrate by a simple protocol that differs from the classical synthesis of pyridine N-oxide. This protocol utilizes different starting material and involves less reaction time to yield a new solvated supramolecular structure, which crystallizes under slow evaporation.
